(Linear) (Six-base) MAPSORT of: KIEE3801.seq  Check: 5305  from: 1  to: 207



KIEE3801.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 16:45  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      73     207 
   Size:         73     134
 
ApoI r'AATT_y

Cuts at:      0      77     207 
   Size:         77     130
 
BsaAI yAC'GTr

Cuts at:      0     117     207 
   Size:        117      90
 
BstYI r'GATC_y

Cuts at:      0       9     207 
   Size:          9     198
 
Bsu36I CC'TnA_GG

Cuts at:      0     161     207 
   Size:        161      46
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      73     207 
   Size:         73     134
 
EcoO109I rG'GnC_Cy

Cuts at:      0      24     207 
   Size:         24     183
 
EcoRI G'AATT_C

Cuts at:      0      77     207 
   Size:         77     130
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     192     207 
   Size:        192      15
 
MspA1I CmG'CkG

Cuts at:      0      51     207 
   Size:         51     156
 
PpuMI rG'GwC_Cy

Cuts at:      0      24     207 
   Size:         24     183
 
PvuII CAG'CTG

Cuts at:      0      51     207 
   Size:         51     156
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      63      95     207 
   Size:         63      32     112

  Fragments arranged by size: 

                 112      63      32
 
XmnI GAAnn'nnTTC

Cuts at:      0     175     207 
   Size:        175      32
 

 Enzymes that do cut:

     AcuI     ApoI    BsaAI    BstYI   Bsu36I  Eco57MI EcoO109I    EcoRI
     FalI   MspA1I    PpuMI    PvuII     TstI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI    EcoNI    EcoRV     FseI     FspI
    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI     MslI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII     TatI
  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI