Restriction map for
pFN21AE3773
(Linear) (Six-base) MAPSORT of: KIEE3773.seq Check: 6774 from: 1 to: 5229
KIEE3773.orf
With 182 enzymes: SgfI *
March 22, 2012 16:45 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 4614 5229
Size: 4614 615
AccI GT'mk_AC
Cuts at: 0 4620 4912 5229
Size: 4620 292 317
Fragments arranged by size:
4620 317 292
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 847 1148 1643 1838 2230 2653 2930 3218
Size: 847 301 495 195 392 423 277 288
Cuts at: 3218 3467 4048 4136 4169 5229
Size: 249 581 88 33 1060
Fragments arranged by size:
1060 847 581 495 423 392 301 288
277 249 195 88 33
AflII C'TTAA_G
Cuts at: 0 747 5229
Size: 747 4482
AflIII A'CryG_T
Cuts at: 0 1635 1962 4671 5229
Size: 1635 327 2709 558
Fragments arranged by size:
2709 1635 558 327
AhdI GACnn_n'nnGTC
Cuts at: 0 1701 5229
Size: 1701 3528
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 4746 5219 5229
Size: 4746 473 10
Fragments arranged by size:
4746 473 10
AlwNI CAG_nnn'CTG
Cuts at: 0 1717 3494 5229
Size: 1717 1777 1735
Fragments arranged by size:
1777 1735 1717
ApaLI G'TGCA_C
Cuts at: 0 2786 5229
Size: 2786 2443
ApoI r'AATT_y
Cuts at: 0 56 442 767 1266 1345 1838 2227 2404
Size: 56 386 325 499 79 493 389 177
Cuts at: 2404 2550 4171 4279 4818 5096 5229
Size: 146 1621 108 539 278 133
Fragments arranged by size:
1621 539 499 493 389 386 325 278
177 146 133 108 79 56
AvaI C'yCGr_G
Cuts at: 0 3394 5229
Size: 3394 1835
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2995 3028 3611 3644 4130 4163 5229
Size: 2995 33 583 33 486 33 1066
Fragments arranged by size:
2995 1066 583 486 33 33 33
BamHI G'GATC_C
Cuts at: 0 18 3681 5229
Size: 18 3663 1548
Fragments arranged by size:
3663 1548 18
BanI G'GyrC_C
Cuts at: 0 2910 5229
Size: 2910 2319
BanII G_rGCy'C
Cuts at: 0 1789 4544 5229
Size: 1789 2755 685
Fragments arranged by size:
2755 1789 685
BbsI GAAGACnn'nnnn_
Cuts at: 0 1304 1483 5229
Size: 1304 179 3746
Fragments arranged by size:
3746 1304 179
BbvCI CC'TCA_GC
Cuts at: 0 1217 1556 3887 5229
Size: 1217 339 2331 1342
Fragments arranged by size:
2331 1342 1217 339
BciVI GTATCCnnnnn_n'
Cuts at: 0 1046 1215 2052 3023 3402 5087 5229
Size: 1046 169 837 971 379 1685 142
Fragments arranged by size:
1685 1046 971 837 379 169 142
BclI T'GATC_A
Cuts at: 0 1629 3111 5229
Size: 1629 1482 2118
Fragments arranged by size:
2118 1629 1482
BfrBI ATG'CAT
Cuts at: 0 1207 5229
Size: 1207 4022
BglI GCCn_nnn'nGGC
Cuts at: 0 3037 5229
Size: 3037 2192
BglII A'GATC_T
Cuts at: 0 4627 5229
Size: 4627 602
BlpI GC'TnA_GC
Cuts at: 0 557 1592 5229
Size: 557 1035 3637
Fragments arranged by size:
3637 1035 557
Bme1580I G_kGCm'C
Cuts at: 0 2790 5229
Size: 2790 2439
BmgBI CAC'GTC
Cuts at: 0 4618 5229
Size: 4618 611
BmrI ACTGGGnnnn_n'
Cuts at: 0 1610 1748 2593 4653 5229
Size: 1610 138 845 2060 576
Fragments arranged by size:
2060 1610 845 576 138
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3675 5229
Size: 3675 1554
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 586 935 2282 2308 2504 3328 4727 5229
Size: 586 349 1347 26 196 824 1399 502
Fragments arranged by size:
1399 1347 824 586 502 349 196 26
Bpu10I CC'TnA_GC
Cuts at: 0 1217 1556 3887 5229
Size: 1217 339 2331 1342
Fragments arranged by size:
2331 1342 1217 339
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3155 5229
Size: 3155 2074
BsaI GGTCTCn'nnnn_
Cuts at: 0 604 1448 5229
Size: 604 844 3781
Fragments arranged by size:
3781 844 604
BsaHI Gr'CG_yC
Cuts at: 0 1465 5229
Size: 1465 3764
BsaWI w'CCGG_w
Cuts at: 0 1073 5229
Size: 1073 4156
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 786 816 2640 2670 4982 5012 5199 5229
Size: 786 30 1824 30 2312 30 187 30
Fragments arranged by size:
2312 1824 786 187 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 479 794 1671 2576 2648 5229
Size: 479 315 877 905 72 2581
Fragments arranged by size:
2581 905 877 479 315 72
BseYI C'CCAG_C
Cuts at: 0 3631 4252 4517 5229
Size: 3631 621 265 712
Fragments arranged by size:
3631 712 621 265
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 780 2975 5215 5229
Size: 780 2195 2240 14
Fragments arranged by size:
2240 2195 780 14
BsiHKAI G_wGCw'C
Cuts at: 0 2790 3374 4544 5229
Size: 2790 584 1170 685
Fragments arranged by size:
2790 1170 685 584
BsmI GAATG_Cn'
Cuts at: 0 1062 1896 2673 5039 5229
Size: 1062 834 777 2366 190
Fragments arranged by size:
2366 1062 834 777 190
Bsp1286I G_dGCh'C
Cuts at: 0 1789 2790 3374 4544 5229
Size: 1789 1001 584 1170 685
Fragments arranged by size:
1789 1170 1001 685 584
BspHI T'CATG_A
Cuts at: 0 731 5229
Size: 731 4498
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 4020 4614 5229
Size: 4020 594 615
Fragments arranged by size:
4020 615 594
BsrBI CCG'CTC
Cuts at: 0 1591 5229
Size: 1591 3638
BsrDI GCAATG_nn'
Cuts at: 0 257 2580 2752 5229
Size: 257 2323 172 2477
Fragments arranged by size:
2477 2323 257 172
BsrGI T'GTAC_A
Cuts at: 0 1959 3516 5229
Size: 1959 1557 1713
Fragments arranged by size:
1959 1713 1557
BstAPI GCAn_nnn'nTGC
Cuts at: 0 789 1213 1717 1923 5229
Size: 789 424 504 206 3306
Fragments arranged by size:
3306 789 504 424 206
BstBI TT'CG_AA
Cuts at: 0 2097 4647 5229
Size: 2097 2550 582
Fragments arranged by size:
2550 2097 582
BstEII G'GTnAC_C
Cuts at: 0 2310 5229
Size: 2310 2919
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2053 4960 5087 5229
Size: 2053 2907 127 142
Fragments arranged by size:
2907 2053 142 127
BstYI r'GATC_y
Cuts at: 0 18 822 862 2447 3681 4213 4627 4698
Size: 18 804 40 1585 1234 532 414 71
Cuts at: 4698 5229
Size: 531
Fragments arranged by size:
1585 1234 804 532 531 414 71 40
18
Bsu36I CC'TnA_GG
Cuts at: 0 1376 5229
Size: 1376 3853
BtgI C'CryG_G
Cuts at: 0 4959 5229
Size: 4959 270
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 3853 5229
Size: 3853 1376
BtsI GCAGTG_nn'
Cuts at: 0 1674 4308 4428 5229
Size: 1674 2634 120 801
Fragments arranged by size:
2634 1674 801 120
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2469 2504 5229
Size: 2469 35 2725
Fragments arranged by size:
2725 2469 35
DraI TTT'AAA
Cuts at: 0 474 726 773 5229
Size: 474 252 47 4456
Fragments arranged by size:
4456 474 252 47
DraIII CAC_nnn'GTG
Cuts at: 0 4708 5229
Size: 4708 521
DrdI GACnn_nn'nnGTC
Cuts at: 0 3798 5229
Size: 3798 1431
EaeI y'GGCC_r
Cuts at: 0 2 1022 2694 5229
Size: 2 1020 1672 2535
Fragments arranged by size:
2535 1672 1020 2
EarI CTCTTCn'nnn_
Cuts at: 0 160 456 2081 2187 2672 3746 4067 4399
Size: 160 296 1625 106 485 1074 321 332
Cuts at: 4399 5229
Size: 830
Fragments arranged by size:
1625 1074 830 485 332 321 296 160
106
EcoICRI GAG'CTC
Cuts at: 0 4542 5229
Size: 4542 687
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 586 847 935 1148 1643 1838 2230 2282
Size: 586 261 88 213 495 195 392 52
Cuts at: 2282 2308 2504 2653 2930 3218 3328 3467 4048
Size: 26 196 149 277 288 110 139 581
Cuts at: 4048 4136 4169 4727 5229
Size: 88 33 558 502
Fragments arranged by size:
586 581 558 502 495 392 288 277
261 213 196 195 149 139 110 88
88 52 33 26
EcoO109I rG'GnC_Cy
Cuts at: 0 698 853 1191 2022 3606 5229
Size: 698 155 338 831 1584 1623
Fragments arranged by size:
1623 1584 831 698 338 155
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 729 2092 5229
Size: 729 1363 3137
Fragments arranged by size:
3137 1363 729
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 713 745 864 896 1246 1278 1687 1719
Size: 713 32 119 32 350 32 409 32
Cuts at: 1719 1773 1805 2606 2638 2640 2670 2672 3442
Size: 54 32 801 32 2 30 2 770
Cuts at: 3442 3474 3745 3777 4417 4449 4929 4961 4982
Size: 32 271 32 640 32 480 32 21
Cuts at: 4982 5014 5229
Size: 32 215
Fragments arranged by size:
801 770 713 640 480 409 350 271
215 119 54 32 32 32 32 32
32 32 32 32 32 32 30 21
2 2
HincII GTy'rAC
Cuts at: 0 335 548 4621 4861 5229
Size: 335 213 4073 240 368
Fragments arranged by size:
4073 368 335 240 213
HindIII A'AGCT_T
Cuts at: 0 274 2100 3550 4367 5229
Size: 274 1826 1450 817 862
Fragments arranged by size:
1826 1450 862 817 274
HpaI GTT'AAC
Cuts at: 0 548 5229
Size: 548 4681
MfeI C'AATT_G
Cuts at: 0 3767 5229
Size: 3767 1462
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2034 2534 3558 5206 5229
Size: 2034 500 1024 1648 23
Fragments arranged by size:
2034 1648 1024 500 23
MscI TGG'CCA
Cuts at: 0 4 1024 5229
Size: 4 1020 4205
Fragments arranged by size:
4205 1020 4
MslI CAynn'nnrTG
Cuts at: 0 898 974 1421 2816 3007 3934 3959 4932
Size: 898 76 447 1395 191 927 25 973
Cuts at: 4932 5229
Size: 297
Fragments arranged by size:
1395 973 927 898 447 297 191 76
25
MspA1I CmG'CkG
Cuts at: 0 2286 2965 3808 3858 5229
Size: 2286 679 843 50 1371
Fragments arranged by size:
2286 1371 843 679 50
NdeI CA'TA_TG
Cuts at: 0 1389 5028 5229
Size: 1389 3639 201
Fragments arranged by size:
3639 1389 201
NsiI A_TGCA'T
Cuts at: 0 1209 5229
Size: 1209 4020
NspI r_CATG'y
Cuts at: 0 1639 1966 4206 4675 5229
Size: 1639 327 2240 469 554
Fragments arranged by size:
2240 1639 554 469 327
PciI A'CATG_T
Cuts at: 0 1635 1962 4671 5229
Size: 1635 327 2709 558
Fragments arranged by size:
2709 1635 558 327
PfoI T'CCnGG_A
Cuts at: 0 3275 5229
Size: 3275 1954
PpuMI rG'GwC_Cy
Cuts at: 0 698 853 1191 2022 3606 5229
Size: 698 155 338 831 1584 1623
Fragments arranged by size:
1623 1584 831 698 338 155
PshAI GACnn'nnGTC
Cuts at: 0 3475 5229
Size: 3475 1754
PstI C_TGCA'G
Cuts at: 0 536 3734 4538 5229
Size: 536 3198 804 691
Fragments arranged by size:
3198 804 691 536
PvuII CAG'CTG
Cuts at: 0 2286 2965 3808 5229
Size: 2286 679 843 1421
Fragments arranged by size:
2286 1421 843 679
SacI G_AGCT'C
Cuts at: 0 4544 5229
Size: 4544 685
SalI G'TCGA_C
Cuts at: 0 4619 5229
Size: 4619 610
SanDI GG'GwC_CC
Cuts at: 0 853 5229
Size: 853 4376
SapI GCTCTTCn'nnn_
Cuts at: 0 2081 2187 4067 5229
Size: 2081 106 1880 1162
Fragments arranged by size:
2081 1880 1162 106
SfcI C'TryA_G
Cuts at: 0 155 410 532 2762 3185 3730 4534 4694
Size: 155 255 122 2230 423 545 804 160
Cuts at: 4694 5120 5229
Size: 426 109
Fragments arranged by size:
2230 804 545 426 423 255 160 155
122 109
SmlI C'TyrA_G
Cuts at: 0 747 3170 5229
Size: 747 2423 2059
Fragments arranged by size:
2423 2059 747
SpeI A'CTAG_T
Cuts at: 0 1084 1857 5229
Size: 1084 773 3372
Fragments arranged by size:
3372 1084 773
SphI G_CATG'C
Cuts at: 0 4206 5229
Size: 4206 1023
SspI AAT'ATT
Cuts at: 0 258 840 5229
Size: 258 582 4389
Fragments arranged by size:
4389 582 258
StuI AGG'CCT
Cuts at: 0 3691 5229
Size: 3691 1538
StyI C'CwwG_G
Cuts at: 0 1968 5229
Size: 1968 3261
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 554 1989 4547 5229
Size: 554 1435 2558 682
Fragments arranged by size:
2558 1435 682 554
TatI w'GTAC_w
Cuts at: 0 1959 2541 3135 3516 5082 5229
Size: 1959 582 594 381 1566 147
Fragments arranged by size:
1959 1566 594 582 381 147
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 786 814 818 846 1220 1252 1513 1545
Size: 786 28 4 28 374 32 261 32
Cuts at: 1545 4008 4040 4298 4330 5199 5229
Size: 2463 32 258 32 869 30
Fragments arranged by size:
2463 869 786 374 261 258 32 32
32 32 30 28 28 4
Tth111I GACn'n_nGTC
Cuts at: 0 2526 2652 5229
Size: 2526 126 2577
Fragments arranged by size:
2577 2526 126
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2606 3273 5229
Size: 2606 667 1956
Fragments arranged by size:
2606 1956 667
XmnI GAAnn'nnTTC
Cuts at: 0 1549 2196 4553 5229
Size: 1549 647 2357 676
Fragments arranged by size:
2357 1549 676 647
Enzymes that do cut:
AarI AccI AcuI AflII AflIII AhdI AlfI AlwNI
ApaLI ApoI AvaI BaeI BamHI BanI BanII BbsI
BbvCI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrGI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI
DraIII DrdI EaeI EarI EcoICRI Eco57MI EcoO109I FalI
Hin4I HincII HindIII HpaI MfeI MmeI MscI MslI
MspA1I NdeI NsiI NspI PciI PfoI PpuMI PshAI
PstI PvuII SacI SalI SanDI SapI SfcI SmlI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AgeI AleI AloI ApaI
AscI AseI AsiSI AvrII BbeI BcgI BmtI BsaAI
BsaBI BsiEI BsiWI BsmBI BspEI BsrFI BssHII BssSI
BstZ17I ClaI EagI EciI EcoNI EcoRI EcoRV FseI
FspI FspAI HaeII KasI KpnI MluI NaeI NarI
NcoI NgoMIV NheI NotI NruI PacI PasI PflMI
PmeI PmlI PpiI PsiI PspOMI PspXI PsrI PvuI
RsrII SacII SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SrfI SwaI XbaI XhoI XmaI
ZraI