Restriction map for
pFN21AE3772
(Linear) (Six-base) MAPSORT of: KIEE3772.seq Check: 5536 from: 1 to: 4818
KIEE3772.orf
With 182 enzymes: SgfI *
July 3, 2012 18:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 733 3931 4818
Size: 733 3198 887
Fragments arranged by size:
3198 887 733
Acc65I G'GTAC_C
Cuts at: 0 878 4818
Size: 878 3940
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 571 1058 2520 2626 2749 2978 3082 4818
Size: 571 487 1462 106 123 229 104 1736
Fragments arranged by size:
1736 1462 571 487 229 123 106 104
AflII C'TTAA_G
Cuts at: 0 3435 4818
Size: 3435 1383
AflIII A'CryG_T
Cuts at: 0 2211 4818
Size: 2211 2607
AhdI GACnn_n'nnGTC
Cuts at: 0 3150 4818
Size: 3150 1668
AleI CACnn'nnGTG
Cuts at: 0 907 2789 4818
Size: 907 1882 2029
Fragments arranged by size:
2029 1882 907
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 751 4612 4818
Size: 751 3861 206
Fragments arranged by size:
3861 751 206
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1392 1424 4700 4732 4818
Size: 1392 32 3276 32 86
Fragments arranged by size:
3276 1392 86 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 2131 3084 3329 3371 4818
Size: 2131 953 245 42 1447
Fragments arranged by size:
2131 1447 953 245 42
ApaLI G'TGCA_C
Cuts at: 0 1294 4605 4818
Size: 1294 3311 213
Fragments arranged by size:
3311 1294 213
ApoI r'AATT_y
Cuts at: 0 256 761 1240 1657 2036 2380 2610 2650
Size: 256 505 479 417 379 344 230 40
Cuts at: 2650 3345 3595 3712 4205 4282 4368 4734 4818
Size: 695 250 117 493 77 86 366 84
Fragments arranged by size:
695 505 493 479 417 379 366 344
256 250 230 117 86 84 77 40
AseI AT'TA_AT
Cuts at: 0 1916 4818
Size: 1916 2902
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 862 895 1109 1142 4001 4034 4818
Size: 862 33 214 33 2859 33 784
Fragments arranged by size:
2859 862 784 214 33 33 33
BanI G'GyrC_C
Cuts at: 0 878 937 4564 4818
Size: 878 59 3627 254
Fragments arranged by size:
3627 878 254 59
BanII G_rGCy'C
Cuts at: 0 92 2525 2950 4818
Size: 92 2433 425 1868
Fragments arranged by size:
2433 1868 425 92
BbeI G_GCGC'C
Cuts at: 0 4568 4818
Size: 4568 250
BbsI GAAGACnn'nnnn_
Cuts at: 0 103 239 1456 1829 2128 2303 3059 3146
Size: 103 136 1217 373 299 175 756 87
Cuts at: 3146 4818
Size: 1672
Fragments arranged by size:
1672 1217 756 373 299 175 136 103
87
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 280 314 1875 1909 4818
Size: 280 34 1561 34 2909
Fragments arranged by size:
2909 1561 280 34 34
BclI T'GATC_A
Cuts at: 0 1184 4818
Size: 1184 3634
BlpI GC'TnA_GC
Cuts at: 0 82 4818
Size: 82 4736
Bme1580I G_kGCm'C
Cuts at: 0 121 1298 2661 4609 4818
Size: 121 1177 1363 1948 209
Fragments arranged by size:
1948 1363 1177 209 121
BmgBI CAC'GTC
Cuts at: 0 1431 4818
Size: 1431 3387
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 76 145 1466 2348 3560 4645 4818
Size: 70 6 69 1321 882 1212 1085 173
Fragments arranged by size:
1321 1212 1085 882 173 70 69 6
Bpu10I CC'TnA_GC
Cuts at: 0 4342 4612 4818
Size: 4342 270 206
Fragments arranged by size:
4342 270 206
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2276 3505 4818
Size: 2276 1229 1313
Fragments arranged by size:
2276 1313 1229
BsaI GGTCTCn'nnnn_
Cuts at: 0 1932 3034 4742 4818
Size: 1932 1102 1708 76
Fragments arranged by size:
1932 1708 1102 76
BsaBI GATnn'nnATC
Cuts at: 0 872 1349 4154 4818
Size: 872 477 2805 664
Fragments arranged by size:
2805 872 664 477
BsaHI Gr'CG_yC
Cuts at: 0 4565 4818
Size: 4565 253
BsaWI w'CCGG_w
Cuts at: 0 425 3686 4751 4818
Size: 425 3261 1065 67
Fragments arranged by size:
3261 1065 425 67
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 3153 3183 4700 4730 4818
Size: 3153 30 1517 30 88
Fragments arranged by size:
3153 1517 88 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 4208 4818
Size: 4208 610
BseYI C'CCAG_C
Cuts at: 0 698 3087 3676 4560 4803 4818
Size: 698 2389 589 884 243 15
Fragments arranged by size:
2389 884 698 589 243 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 188 711 1188 2767 3089 3405 4605 4751
Size: 188 523 477 1579 322 316 1200 146
Cuts at: 4751 4818
Size: 67
Fragments arranged by size:
1579 1200 523 477 322 316 188 146
67
BsiHKAI G_wGCw'C
Cuts at: 0 83 92 1298 2014 2292 4609 4818
Size: 83 9 1206 716 278 2317 209
Fragments arranged by size:
2317 1206 716 278 209 83 9
BsmI GAATG_Cn'
Cuts at: 0 142 283 3942 4348 4818
Size: 142 141 3659 406 470
Fragments arranged by size:
3659 470 406 142 141
Bsp1286I G_dGCh'C
Cuts at: 0 83 92 121 1298 2014 2292 2525 2661
Size: 83 9 29 1177 716 278 233 136
Cuts at: 2661 2950 4609 4818
Size: 289 1659 209
Fragments arranged by size:
1659 1177 716 289 278 233 209 136
83 29 9
BspEI T'CCGG_A
Cuts at: 0 425 3686 4818
Size: 425 3261 1132
Fragments arranged by size:
3261 1132 425
BspHI T'CATG_A
Cuts at: 0 1127 2907 3294 3453 4818
Size: 1127 1780 387 159 1365
Fragments arranged by size:
1780 1365 1127 387 159
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 328 733 3931 4818
Size: 328 405 3198 887
Fragments arranged by size:
3198 887 405 328
BsrBI CCG'CTC
Cuts at: 0 105 4818
Size: 105 4713
BsrDI GCAATG_nn'
Cuts at: 0 1601 2204 2505 4818
Size: 1601 603 301 2313
Fragments arranged by size:
2313 1601 603 301
BsrGI T'GTAC_A
Cuts at: 0 2589 2957 4778 4818
Size: 2589 368 1821 40
Fragments arranged by size:
2589 1821 368 40
BssSI C'ACGA_G
Cuts at: 0 2720 4818
Size: 2720 2098
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2344 2495 3383 4818
Size: 2344 151 888 1435
Fragments arranged by size:
2344 1435 888 151
BstEII G'GTnAC_C
Cuts at: 0 1921 4818
Size: 1921 2897
BstXI CCAn_nnnn'nTGG
Cuts at: 0 48 958 4641 4818
Size: 48 910 3683 177
Fragments arranged by size:
3683 910 177 48
BstYI r'GATC_y
Cuts at: 0 1864 2069 3660 4471 4818
Size: 1864 205 1591 811 347
Fragments arranged by size:
1864 1591 811 347 205
Bsu36I CC'TnA_GG
Cuts at: 0 1283 4818
Size: 1283 3535
BtgI C'CryG_G
Cuts at: 0 15 562 2981 3024 4653 4818
Size: 15 547 2419 43 1629 165
Fragments arranged by size:
2419 1629 547 165 43 15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 3336 4818
Size: 3336 1482
BtsI GCAGTG_nn'
Cuts at: 0 593 963 2673 2779 2796 4515 4818
Size: 593 370 1710 106 17 1719 303
Fragments arranged by size:
1719 1710 593 370 303 106 17
DraI TTT'AAA
Cuts at: 0 336 1097 2754 4056 4203 4818
Size: 336 761 1657 1302 147 615
Fragments arranged by size:
1657 1302 761 615 336 147
DrdI GACnn_nn'nnGTC
Cuts at: 0 4761 4818
Size: 4761 57
EaeI y'GGCC_r
Cuts at: 0 891 1535 2239 2393 3021 3815 4643 4818
Size: 891 644 704 154 628 794 828 175
Fragments arranged by size:
891 828 794 704 644 628 175 154
EarI CTCTTCn'nnn_
Cuts at: 0 1034 1583 2103 2360 3716 3755 4061 4818
Size: 1034 549 520 257 1356 39 306 757
Fragments arranged by size:
1356 1034 757 549 520 306 257 39
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1101 4818
Size: 1101 3717
EcoICRI GAG'CTC
Cuts at: 0 90 4818
Size: 90 4728
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 76 145 571 1058 1466 2348 2520
Size: 70 6 69 426 487 408 882 172
Cuts at: 2520 2626 2749 2978 3082 3560 4645 4818
Size: 106 123 229 104 478 1085 173
Fragments arranged by size:
1085 882 487 478 426 408 229 173
172 123 106 104 70 69 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1286 4818
Size: 1286 3532
EcoO109I rG'GnC_Cy
Cuts at: 0 45 1514 2738 3548 4818
Size: 45 1469 1224 810 1270
Fragments arranged by size:
1469 1270 1224 810 45
EcoRI G'AATT_C
Cuts at: 0 256 1657 3345 4818
Size: 256 1401 1688 1473
Fragments arranged by size:
1688 1473 1401 256
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 256 2317 4062 4818
Size: 256 2061 1745 756
Fragments arranged by size:
2061 1745 756 256
HaeII r_GCGC'y
Cuts at: 0 41 4568 4818
Size: 41 4527 250
Fragments arranged by size:
4527 250 41
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 3136 3168 3681 3713 4818
Size: 3136 32 513 32 1105
Fragments arranged by size:
3136 1105 513 32 32
HincII GTy'rAC
Cuts at: 0 183 4818
Size: 183 4635
KasI G'GCGC_C
Cuts at: 0 4564 4818
Size: 4564 254
KpnI G_GTAC'C
Cuts at: 0 882 4818
Size: 882 3936
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 650 734 1238 1590 2864 4503 4818
Size: 650 84 504 352 1274 1639 315
Fragments arranged by size:
1639 1274 650 504 352 315 84
MscI TGG'CCA
Cuts at: 0 1537 2395 3023 3817 4645 4818
Size: 1537 858 628 794 828 173
Fragments arranged by size:
1537 858 828 794 628 173
MslI CAynn'nnrTG
Cuts at: 0 733 907 956 2198 2789 2819 2980 3404
Size: 733 174 49 1242 591 30 161 424
Cuts at: 3404 4579 4818
Size: 1175 239
Fragments arranged by size:
1242 1175 733 591 424 239 174 161
49 30
MspA1I CmG'CkG
Cuts at: 0 17 943 2050 2131 2485 4486 4592 4807
Size: 17 926 1107 81 354 2001 106 215
Cuts at: 4807 4818
Size: 11
Fragments arranged by size:
2001 1107 926 354 215 106 81 17
11
NarI GG'CG_CC
Cuts at: 0 4565 4818
Size: 4565 253
NcoI C'CATG_G
Cuts at: 0 2981 3024 4818
Size: 2981 43 1794
Fragments arranged by size:
2981 1794 43
NspI r_CATG'y
Cuts at: 0 450 2101 2197 2422 4818
Size: 450 1651 96 225 2396
Fragments arranged by size:
2396 1651 450 225 96
PflMI CCAn_nnn'nTGG
Cuts at: 0 2264 4818
Size: 2264 2554
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 4700 4732 4818
Size: 4700 32 86
Fragments arranged by size:
4700 86 32
PpuMI rG'GwC_Cy
Cuts at: 0 45 1514 4818
Size: 45 1469 3304
Fragments arranged by size:
3304 1469 45
PshAI GACnn'nnGTC
Cuts at: 0 4530 4818
Size: 4530 288
PsiI TTA'TAA
Cuts at: 0 1157 2273 2708 4818
Size: 1157 1116 435 2110
Fragments arranged by size:
2110 1157 1116 435
PstI C_TGCA'G
Cuts at: 0 2127 2227 3376 4818
Size: 2127 100 1149 1442
Fragments arranged by size:
2127 1442 1149 100
PvuII CAG'CTG
Cuts at: 0 2050 2131 2485 4486 4807 4818
Size: 2050 81 354 2001 321 11
Fragments arranged by size:
2050 2001 354 321 81 11
SacI G_AGCT'C
Cuts at: 0 92 4818
Size: 92 4726
SacII CC_GC'GG
Cuts at: 0 18 4818
Size: 18 4800
SapI GCTCTTCn'nnn_
Cuts at: 0 3755 4818
Size: 3755 1063
SbfI CC_TGCA'GG
Cuts at: 0 2227 4818
Size: 2227 2591
SfcI C'TryA_G
Cuts at: 0 863 2102 2123 2223 3372 4685 4818
Size: 863 1239 21 100 1149 1313 133
Fragments arranged by size:
1313 1239 1149 863 133 100 21
SfoI GGC'GCC
Cuts at: 0 4566 4818
Size: 4566 252
SmlI C'TyrA_G
Cuts at: 0 2291 3435 3484 4818
Size: 2291 1144 49 1334
Fragments arranged by size:
2291 1334 1144 49
SphI G_CATG'C
Cuts at: 0 450 2101 4818
Size: 450 1651 2717
Fragments arranged by size:
2717 1651 450
SspI AAT'ATT
Cuts at: 0 364 777 3760 4818
Size: 364 413 2983 1058
Fragments arranged by size:
2983 1058 413 364
StuI AGG'CCT
Cuts at: 0 1281 4422 4818
Size: 1281 3141 396
Fragments arranged by size:
3141 1281 396
StyI C'CwwG_G
Cuts at: 0 41 482 2981 3024 3664 4771 4818
Size: 41 441 2499 43 640 1107 47
Fragments arranged by size:
2499 1107 640 441 47 43 41
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 115 1208 4818
Size: 115 1093 3610
Fragments arranged by size:
3610 1093 115
TatI w'GTAC_w
Cuts at: 0 246 818 2231 2589 2724 2957 4328 4778
Size: 246 572 1413 358 135 233 1371 450
Cuts at: 4778 4818
Size: 40
Fragments arranged by size:
1413 1371 572 450 358 246 233 135
40
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 462 494 558 590 4818
Size: 462 32 64 32 4228
Fragments arranged by size:
4228 462 64 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 2440 4818
Size: 2440 2378
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 648 2452 2979 4818
Size: 648 1804 527 1839
Fragments arranged by size:
1839 1804 648 527
XmnI GAAnn'nnTTC
Cuts at: 0 2300 2627 4169 4283 4818
Size: 2300 327 1542 114 535
Fragments arranged by size:
2300 1542 535 327 114
Enzymes that do cut:
AarI Acc65I AcuI AflII AflIII AhdI AleI AlfI
AloI AlwNI ApaLI ApoI AseI BaeI BanI BanII
BbeI BbsI BcgI BclI BlpI Bme1580I BmgBI BpmI
Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrGI BssSI BstAPI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII
Hin4I HincII KasI KpnI MmeI MscI MslI MspA1I
NarI NcoI NspI PflMI PpiI PpuMI PshAI PsiI
PstI PvuII SacI SacII SapI SbfI SfcI SfoI
SmlI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XcmI XmnI
Enzymes that do not cut:
AatII AccI AclI AfeI AgeI ApaI AscI AsiSI
AvaI AvrII BamHI BbvCI BciVI BfrBI BglI BglII
BmrI BmtI BplI BsaAI BsiEI BsiWI BsmBI BsrFI
BssHII BstBI BstZ17I ClaI CspCI DraIII EagI EcoRV
FseI FspI FspAI HindIII HpaI MfeI MluI NaeI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PciI PfoI PmeI PmlI PspOMI PspXI PsrI PvuI
RsrII SalI SanDI ScaI SexAI SfiI SgfI SgrAI
SmaI SnaBI SpeI SrfI SwaI XbaI XhoI XmaI
ZraI