Restriction map for
pFN21AE3770
(Linear) (Six-base) MAPSORT of: KIEE3770.seq Check: 3416 from: 1 to: 2982
KIEE3770.orf
With 182 enzymes: SgfI *
March 22, 2012 16:44 ..
AatII G_ACGT'C
Cuts at: 0 1339 2982
Size: 1339 1643
AccI GT'mk_AC
Cuts at: 0 687 2982
Size: 687 2295
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 330 388 1829 2034 2982
Size: 330 58 1441 205 948
Fragments arranged by size:
1441 948 330 205 58
AleI CACnn'nnGTG
Cuts at: 0 661 2982
Size: 661 2321
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 304 2982
Size: 304 2678
AlwNI CAG_nnn'CTG
Cuts at: 0 2031 2982
Size: 2031 951
ApaI G_GGCC'C
Cuts at: 0 48 848 2835 2982
Size: 48 800 1987 147
Fragments arranged by size:
1987 800 147 48
ApaLI G'TGCA_C
Cuts at: 0 1158 2848 2982
Size: 1158 1690 134
Fragments arranged by size:
1690 1158 134
ApoI r'AATT_y
Cuts at: 0 2207 2982
Size: 2207 775
AvaI C'yCGr_G
Cuts at: 0 41 47 142 2583 2982
Size: 41 6 95 2441 399
Fragments arranged by size:
2441 399 95 41 6
BamHI G'GATC_C
Cuts at: 0 215 2982
Size: 215 2767
BanI G'GyrC_C
Cuts at: 0 8 71 1108 1932 2557 2982
Size: 8 63 1037 824 625 425
Fragments arranged by size:
1037 824 625 425 63 8
BanII G_rGCy'C
Cuts at: 0 48 848 2835 2982
Size: 48 800 1987 147
Fragments arranged by size:
1987 800 147 48
BbeI G_GCGC'C
Cuts at: 0 12 2982
Size: 12 2970
BbsI GAAGACnn'nnnn_
Cuts at: 0 950 1096 1700 2185 2982
Size: 950 146 604 485 797
Fragments arranged by size:
950 797 604 485 146
BbvCI CC'TCA_GC
Cuts at: 0 1681 2982
Size: 1681 1301
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 493 527 2982
Size: 493 34 2455
Fragments arranged by size:
2455 493 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 239 415 1511 2982
Size: 239 176 1096 1471
Fragments arranged by size:
1471 1096 239 176
BclI T'GATC_A
Cuts at: 0 1925 2982
Size: 1925 1057
BfrBI ATG'CAT
Cuts at: 0 439 2128 2226 2982
Size: 439 1689 98 756
Fragments arranged by size:
1689 756 439 98
Bme1580I G_kGCm'C
Cuts at: 0 48 848 1162 1321 2560 2835 2852 2982
Size: 48 800 314 159 1239 275 17 130
Fragments arranged by size:
1239 800 314 275 159 130 48 17
BmtI G_CTAG'C
Cuts at: 0 2762 2982
Size: 2762 220
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 358 483 951 2128 2545 2962 2982
Size: 358 125 468 1177 417 417 20
Fragments arranged by size:
1177 468 417 417 358 125 20
Bpu10I CC'TnA_GC
Cuts at: 0 211 1681 1791 1874 2402 2982
Size: 211 1470 110 83 528 580
Fragments arranged by size:
1470 580 528 211 110 83
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2568 2982
Size: 2568 414
BsaI GGTCTCn'nnnn_
Cuts at: 0 517 2982
Size: 517 2465
BsaAI yAC'GTr
Cuts at: 0 426 2115 2982
Size: 426 1689 867
Fragments arranged by size:
1689 867 426
BsaHI Gr'CG_yC
Cuts at: 0 9 1336 2982
Size: 9 1327 1646
Fragments arranged by size:
1646 1327 9
BsaWI w'CCGG_w
Cuts at: 0 921 2982
Size: 921 2061
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1346 1780 2982
Size: 1346 434 1202
Fragments arranged by size:
1346 1202 434
BseYI C'CCAG_C
Cuts at: 0 679 1360 2038 2982
Size: 679 681 678 944
Fragments arranged by size:
944 681 679 678
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 746 1059 1222 2396 2929 2982
Size: 746 313 163 1174 533 53
Fragments arranged by size:
1174 746 533 313 163 53
BsiEI CG_ry'CG
Cuts at: 0 84 2982
Size: 84 2898
BsiHKAI G_wGCw'C
Cuts at: 0 1162 2114 2578 2852 2982
Size: 1162 952 464 274 130
Fragments arranged by size:
1162 952 464 274 130
BsmBI CGTCTCn'nnnn_
Cuts at: 0 46 2982
Size: 46 2936
Bsp1286I G_dGCh'C
Cuts at: 0 48 848 1162 1321 2114 2560 2578 2835
Size: 48 800 314 159 793 446 18 257
Cuts at: 2835 2852 2982
Size: 17 130
Fragments arranged by size:
800 793 446 314 257 159 130 48
18 17
BspEI T'CCGG_A
Cuts at: 0 921 2982
Size: 921 2061
BspHI T'CATG_A
Cuts at: 0 470 807 2982
Size: 470 337 2175
Fragments arranged by size:
2175 470 337
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2951 2982
Size: 2951 31
BsrDI GCAATG_nn'
Cuts at: 0 775 2982
Size: 775 2207
BsrFI r'CCGG_y
Cuts at: 0 59 338 2982
Size: 59 279 2644
Fragments arranged by size:
2644 279 59
BsrGI T'GTAC_A
Cuts at: 0 1776 2982
Size: 1776 1206
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2031 2982
Size: 2031 951
BstXI CCAn_nnnn'nTGG
Cuts at: 0 663 2982
Size: 663 2319
BstYI r'GATC_y
Cuts at: 0 215 377 2982
Size: 215 162 2605
Fragments arranged by size:
2605 215 162
BstZ17I GTA'TAC
Cuts at: 0 688 2982
Size: 688 2294
Bsu36I CC'TnA_GG
Cuts at: 0 1016 1195 1276 2982
Size: 1016 179 81 1706
Fragments arranged by size:
1706 1016 179 81
BtgI C'CryG_G
Cuts at: 0 417 840 2982
Size: 417 423 2142
Fragments arranged by size:
2142 423 417
BtsI GCAGTG_nn'
Cuts at: 0 1076 2982
Size: 1076 1906
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 224 259 2982
Size: 224 35 2723
Fragments arranged by size:
2723 224 35
DraI TTT'AAA
Cuts at: 0 2187 2982
Size: 2187 795
EaeI y'GGCC_r
Cuts at: 0 92 491 1295 1956 2982
Size: 92 399 804 661 1026
Fragments arranged by size:
1026 804 661 399 92
EarI CTCTTCn'nnn_
Cuts at: 0 349 560 1551 2053 2982
Size: 349 211 991 502 929
Fragments arranged by size:
991 929 502 349 211
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 41 2982
Size: 41 2941
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 330 358 388 483 951 1829 2034 2128
Size: 330 28 30 95 468 878 205 94
Cuts at: 2128 2545 2962 2982
Size: 417 417 20
Fragments arranged by size:
878 468 417 417 330 205 95 94
30 28 20
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1373 2283 2982
Size: 1373 910 699
Fragments arranged by size:
1373 910 699
EcoO109I rG'GnC_Cy
Cuts at: 0 299 822 845 1820 2822 2831 2982
Size: 299 523 23 975 1002 9 151
Fragments arranged by size:
1002 975 523 299 151 23 9
EcoRV GAT'ATC
Cuts at: 0 609 2982
Size: 609 2373
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2199 2982
Size: 2199 783
HaeII r_GCGC'y
Cuts at: 0 12 1220 2031 2982
Size: 12 1208 811 951
Fragments arranged by size:
1208 951 811 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 514 546 618 650 955 987 1191 1223
Size: 514 32 72 32 305 32 204 32
Cuts at: 1223 1664 1696 2982
Size: 441 32 1286
Fragments arranged by size:
1286 514 441 305 204 72 32 32
32 32 32
KasI G'GCGC_C
Cuts at: 0 8 2982
Size: 8 2974
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 256 434 776 2087 2982
Size: 256 178 342 1311 895
Fragments arranged by size:
1311 895 342 256 178
MscI TGG'CCA
Cuts at: 0 493 1297 1958 2982
Size: 493 804 661 1024
Fragments arranged by size:
1024 804 661 493
MslI CAynn'nnrTG
Cuts at: 0 661 959 1127 1246 2372 2982
Size: 661 298 168 119 1126 610
Fragments arranged by size:
1126 661 610 298 168 119
MspA1I CmG'CkG
Cuts at: 0 80 107 1536 2982
Size: 80 27 1429 1446
Fragments arranged by size:
1446 1429 80 27
NaeI GCC'GGC
Cuts at: 0 61 2982
Size: 61 2921
NarI GG'CG_CC
Cuts at: 0 9 2982
Size: 9 2973
NcoI C'CATG_G
Cuts at: 0 840 2982
Size: 840 2142
NgoMIV G'CCGG_C
Cuts at: 0 59 2982
Size: 59 2923
NheI G'CTAG_C
Cuts at: 0 2758 2982
Size: 2758 224
NsiI A_TGCA'T
Cuts at: 0 441 2130 2228 2982
Size: 441 1689 98 754
Fragments arranged by size:
1689 754 441 98
NspI r_CATG'y
Cuts at: 0 1085 1245 1701 1777 2128 2230 2249 2982
Size: 1085 160 456 76 351 102 19 733
Fragments arranged by size:
1085 733 456 351 160 102 76 19
PasI CC'CwG_GG
Cuts at: 0 2701 2982
Size: 2701 281
PflMI CCAn_nnn'nTGG
Cuts at: 0 390 1414 2982
Size: 390 1024 1568
Fragments arranged by size:
1568 1024 390
PfoI T'CCnGG_A
Cuts at: 0 1609 2564 2982
Size: 1609 955 418
Fragments arranged by size:
1609 955 418
PmlI CAC'GTG
Cuts at: 0 2115 2982
Size: 2115 867
PpuMI rG'GwC_Cy
Cuts at: 0 299 822 2822 2982
Size: 299 523 2000 160
Fragments arranged by size:
2000 523 299 160
PshAI GACnn'nnGTC
Cuts at: 0 815 2452 2982
Size: 815 1637 530
Fragments arranged by size:
1637 815 530
PspOMI G'GGCC_C
Cuts at: 0 44 844 2831 2982
Size: 44 800 1987 151
Fragments arranged by size:
1987 800 151 44
PspXI vC'TCGA_Gb
Cuts at: 0 2583 2982
Size: 2583 399
PstI C_TGCA'G
Cuts at: 0 434 634 1741 2024 2982
Size: 434 200 1107 283 958
Fragments arranged by size:
1107 958 434 283 200
PvuII CAG'CTG
Cuts at: 0 107 2982
Size: 107 2875
SanDI GG'GwC_CC
Cuts at: 0 299 2982
Size: 299 2683
SapI GCTCTTCn'nnn_
Cuts at: 0 349 560 2982
Size: 349 211 2422
Fragments arranged by size:
2422 349 211
SfcI C'TryA_G
Cuts at: 0 120 430 533 630 1347 1705 1737 2020
Size: 120 310 103 97 717 358 32 283
Cuts at: 2020 2982
Size: 962
Fragments arranged by size:
962 717 358 310 283 120 103 97
32
SfoI GGC'GCC
Cuts at: 0 10 2982
Size: 10 2972
SmaI CCC'GGG
Cuts at: 0 43 49 144 2982
Size: 43 6 95 2838
Fragments arranged by size:
2838 95 43 6
SmlI C'TyrA_G
Cuts at: 0 2547 2583 2982
Size: 2547 36 399
Fragments arranged by size:
2547 399 36
SnaBI TAC'GTA
Cuts at: 0 426 2982
Size: 426 2556
SphI G_CATG'C
Cuts at: 0 2128 2982
Size: 2128 854
SrfI GCCC'GGGC
Cuts at: 0 43 2982
Size: 43 2939
StuI AGG'CCT
Cuts at: 0 1353 1735 2982
Size: 1353 382 1247
Fragments arranged by size:
1353 1247 382
StyI C'CwwG_G
Cuts at: 0 389 840 1047 1936 2982
Size: 389 451 207 889 1046
Fragments arranged by size:
1046 889 451 389 207
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 177 967 2982
Size: 177 790 2015
Fragments arranged by size:
2015 790 177
TatI w'GTAC_w
Cuts at: 0 109 1776 2982
Size: 109 1667 1206
Fragments arranged by size:
1667 1206 109
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 178 2070 2982
Size: 178 1892 912
Fragments arranged by size:
1892 912 178
XhoI C'TCGA_G
Cuts at: 0 2583 2982
Size: 2583 399
XmaI C'CCGG_G
Cuts at: 0 41 47 142 2982
Size: 41 6 95 2840
Fragments arranged by size:
2840 95 41 6
XmnI GAAnn'nnTTC
Cuts at: 0 1093 1801 2982
Size: 1093 708 1181
Fragments arranged by size:
1181 1093 708
ZraI GAC'GTC
Cuts at: 0 1337 2982
Size: 1337 1645
Enzymes that do cut:
AatII AccI AcuI AleI AlfI AlwNI ApaI ApaLI
ApoI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI Bme1580I BmtI BpmI Bpu10I
BpuEI BsaI BsaAI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrDI
BsrFI BsrGI BstAPI BstXI BstYI BstZ17I Bsu36I BtgI
BtsI CspCI DraI EaeI EarI EciI Eco57MI EcoNI
EcoO109I EcoRV FalI HaeII Hin4I KasI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NheI NsiI
NspI PasI PflMI PfoI PmlI PpuMI PshAI PspOMI
PspXI PstI PvuII SanDI SapI SfcI SfoI SmaI
SmlI SnaBI SphI SrfI StuI StyI TaqII TatI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AflIII AgeI AhdI
AloI AscI AseI AsiSI AvrII BaeI BglI BglII
BlpI BmgBI BmrI BplI BsaBI BsaXI BsiWI BsmI
BsrBI BssHII BssSI BstBI BstEII BtgZI ClaI DraIII
DrdI EagI EcoICRI EcoRI FseI FspI FspAI HincII
HindIII HpaI KpnI MfeI MluI NdeI NotI NruI
PacI PciI PmeI PpiI PsiI PsrI PvuI RsrII
SacI SacII SalI SbfI ScaI SexAI SfiI SgfI
SgrAI SpeI SspI SwaI TstI Tth111I XbaI