Restriction map for
pFN21AE3769
(Linear) (Six-base) MAPSORT of: KIEE3769.seq Check: 2486 from: 1 to: 2748
KIEE3769.orf
With 182 enzymes: SgfI *
July 3, 2012 18:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 796 2748
Size: 796 1952
AccI GT'mk_AC
Cuts at: 0 1604 2748
Size: 1604 1144
AclI AA'CG_TT
Cuts at: 0 2337 2748
Size: 2337 411
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 439 1138 1593 1968 2748
Size: 439 699 455 375 780
Fragments arranged by size:
780 699 455 439 375
AfeI AGC'GCT
Cuts at: 0 1655 2748
Size: 1655 1093
AflIII A'CryG_T
Cuts at: 0 164 2408 2561 2748
Size: 164 2244 153 187
Fragments arranged by size:
2244 187 164 153
AhdI GACnn_n'nnGTC
Cuts at: 0 2301 2748
Size: 2301 447
AleI CACnn'nnGTG
Cuts at: 0 448 1570 2748
Size: 448 1122 1178
Fragments arranged by size:
1178 1122 448
AlwNI CAG_nnn'CTG
Cuts at: 0 232 1047 2357 2748
Size: 232 815 1310 391
Fragments arranged by size:
1310 815 391 232
ApoI r'AATT_y
Cuts at: 0 1493 2019 2386 2635 2748
Size: 1493 526 367 249 113
Fragments arranged by size:
1493 526 367 249 113
AvaI C'yCGr_G
Cuts at: 0 1532 2748
Size: 1532 1216
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 312 345 2748
Size: 312 33 2403
Fragments arranged by size:
2403 312 33
BanI G'GyrC_C
Cuts at: 0 969 2748
Size: 969 1779
BanII G_rGCy'C
Cuts at: 0 761 2210 2748
Size: 761 1449 538
Fragments arranged by size:
1449 761 538
BbsI GAAGACnn'nnnn_
Cuts at: 0 2182 2748
Size: 2182 566
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2443 2477 2748
Size: 2443 34 271
Fragments arranged by size:
2443 271 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1197 2748
Size: 1197 1551
BglI GCCn_nnn'nGGC
Cuts at: 0 967 2748
Size: 967 1781
BglII A'GATC_T
Cuts at: 0 1080 2748
Size: 1080 1668
BmrI ACTGGGnnnn_n'
Cuts at: 0 1919 2748
Size: 1919 829
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1440 2748
Size: 1440 1308
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1410 1797 2379 2748
Size: 1410 387 582 369
Fragments arranged by size:
1410 582 387 369
Bpu10I CC'TnA_GC
Cuts at: 0 1253 2748
Size: 1253 1495
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 974 1726 2748
Size: 974 752 1022
Fragments arranged by size:
1022 974 752
BsaI GGTCTCn'nnnn_
Cuts at: 0 1924 2218 2748
Size: 1924 294 530
Fragments arranged by size:
1924 530 294
BsaHI Gr'CG_yC
Cuts at: 0 959 2748
Size: 959 1789
BsaWI w'CCGG_w
Cuts at: 0 30 2748
Size: 30 2718
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1606 1636 2748
Size: 1606 30 1112
Fragments arranged by size:
1606 1112 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 998 2748
Size: 998 1750
BseYI C'CCAG_C
Cuts at: 0 158 613 2748
Size: 158 455 2135
Fragments arranged by size:
2135 455 158
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 812 2748
Size: 812 1936
BsiEI CG_ry'CG
Cuts at: 0 31 2748
Size: 31 2717
BsiHKAI G_wGCw'C
Cuts at: 0 2210 2748
Size: 2210 538
Bsp1286I G_dGCh'C
Cuts at: 0 761 2210 2748
Size: 761 1449 538
Fragments arranged by size:
1449 761 538
BspHI T'CATG_A
Cuts at: 0 794 2748
Size: 794 1954
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 796 2748
Size: 796 1952
BsrDI GCAATG_nn'
Cuts at: 0 482 1439 1812 2459 2748
Size: 482 957 373 647 289
Fragments arranged by size:
957 647 482 373 289
BsrFI r'CCGG_y
Cuts at: 0 116 2748
Size: 116 2632
BsrGI T'GTAC_A
Cuts at: 0 167 2748
Size: 167 2581
BstAPI GCAn_nnn'nTGC
Cuts at: 0 684 2748
Size: 684 2064
BstXI CCAn_nnnn'nTGG
Cuts at: 0 613 2319 2748
Size: 613 1706 429
Fragments arranged by size:
1706 613 429
BstYI r'GATC_y
Cuts at: 0 228 697 1080 2315 2748
Size: 228 469 383 1235 433
Fragments arranged by size:
1235 469 433 383 228
BtgI C'CryG_G
Cuts at: 0 1212 1571 2075 2219 2486 2748
Size: 1212 359 504 144 267 262
Fragments arranged by size:
1212 504 359 267 262 144
BtsI GCAGTG_nn'
Cuts at: 0 103 136 387 817 824 1297 1353 1902
Size: 103 33 251 430 7 473 56 549
Cuts at: 1902 2504 2748
Size: 602 244
Fragments arranged by size:
602 549 473 430 251 244 103 56
33 7
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1286 1321 2748
Size: 1286 35 1427
Fragments arranged by size:
1427 1286 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 48 601 687 2748
Size: 48 553 86 2061
Fragments arranged by size:
2061 553 86 48
EaeI y'GGCC_r
Cuts at: 0 1135 2072 2496 2748
Size: 1135 937 424 252
Fragments arranged by size:
1135 937 424 252
EarI CTCTTCn'nnn_
Cuts at: 0 1225 2215 2748
Size: 1225 990 533
Fragments arranged by size:
1225 990 533
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 2748
Size: 19 2729
EcoICRI GAG'CTC
Cuts at: 0 2208 2748
Size: 2208 540
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 439 1138 1410 1593 1797 1968 2379 2748
Size: 439 699 272 183 204 171 411 369
Fragments arranged by size:
699 439 411 369 272 204 183 171
EcoNI CCTnn'n_nnAGG
Cuts at: 0 69 1414 2748
Size: 69 1345 1334
Fragments arranged by size:
1345 1334 69
EcoO109I rG'GnC_Cy
Cuts at: 0 1799 2748
Size: 1799 949
EcoRI G'AATT_C
Cuts at: 0 2386 2748
Size: 2386 362
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1125 1894 2748
Size: 1125 769 854
Fragments arranged by size:
1125 854 769
HaeII r_GCGC'y
Cuts at: 0 1657 2748
Size: 1657 1091
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 721 753 1606 1638 2107 2139 2186 2218
Size: 721 32 853 32 469 32 47 32
Cuts at: 2218 2287 2319 2748
Size: 69 32 429
Fragments arranged by size:
853 721 469 429 69 47 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 1700 2748
Size: 1700 1048
HpaI GTT'AAC
Cuts at: 0 1700 2748
Size: 1700 1048
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 108 1430 2748
Size: 108 1322 1318
Fragments arranged by size:
1322 1318 108
MscI TGG'CCA
Cuts at: 0 2074 2498 2748
Size: 2074 424 250
Fragments arranged by size:
2074 424 250
MslI CAynn'nnrTG
Cuts at: 0 448 1546 1570 2218 2464 2748
Size: 448 1098 24 648 246 284
Fragments arranged by size:
1098 648 448 284 246 24
MspA1I CmG'CkG
Cuts at: 0 158 1389 1823 2011 2032 2748
Size: 158 1231 434 188 21 716
Fragments arranged by size:
1231 716 434 188 158 21
NcoI C'CATG_G
Cuts at: 0 1212 2075 2219 2486 2748
Size: 1212 863 144 267 262
Fragments arranged by size:
1212 863 267 262 144
NdeI CA'TA_TG
Cuts at: 0 2511 2748
Size: 2511 237
NspI r_CATG'y
Cuts at: 0 168 981 2146 2154 2412 2748
Size: 168 813 1165 8 258 336
Fragments arranged by size:
1165 813 336 258 168 8
PciI A'CATG_T
Cuts at: 0 164 2408 2748
Size: 164 2244 340
Fragments arranged by size:
2244 340 164
PflMI CCAn_nnn'nTGG
Cuts at: 0 2081 2748
Size: 2081 667
PfoI T'CCnGG_A
Cuts at: 0 605 1813 2748
Size: 605 1208 935
Fragments arranged by size:
1208 935 605
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 407 439 2748
Size: 407 32 2309
Fragments arranged by size:
2309 407 32
PsiI TTA'TAA
Cuts at: 0 2642 2748
Size: 2642 106
PstI C_TGCA'G
Cuts at: 0 1360 1449 1564 2748
Size: 1360 89 115 1184
Fragments arranged by size:
1360 1184 115 89
PvuII CAG'CTG
Cuts at: 0 158 1823 2011 2032 2748
Size: 158 1665 188 21 716
Fragments arranged by size:
1665 716 188 158 21
SacI G_AGCT'C
Cuts at: 0 2210 2748
Size: 2210 538
SapI GCTCTTCn'nnn_
Cuts at: 0 2215 2748
Size: 2215 533
SfcI C'TryA_G
Cuts at: 0 1356 1445 1560 2748
Size: 1356 89 115 1188
Fragments arranged by size:
1356 1188 115 89
SmlI C'TyrA_G
Cuts at: 0 989 1705 2748
Size: 989 716 1043
Fragments arranged by size:
1043 989 716
StuI AGG'CCT
Cuts at: 0 2131 2442 2748
Size: 2131 311 306
Fragments arranged by size:
2131 311 306
StyI C'CwwG_G
Cuts at: 0 1212 1787 2075 2219 2229 2486 2748
Size: 1212 575 288 144 10 257 262
Fragments arranged by size:
1212 575 288 262 257 144 10
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 166 1951 2748
Size: 166 1785 797
Fragments arranged by size:
1785 797 166
TatI w'GTAC_w
Cuts at: 0 167 2748
Size: 167 2581
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1809 1841 2748
Size: 1809 32 907
Fragments arranged by size:
1809 907 32
Tth111I GACn'n_nGTC
Cuts at: 0 2377 2748
Size: 2377 371
XbaI T'CTAG_A
Cuts at: 0 1062 2748
Size: 1062 1686
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2319 2748
Size: 2319 429
XmnI GAAnn'nnTTC
Cuts at: 0 514 2748
Size: 514 2234
Enzymes that do cut:
AarI AccI AclI AcuI AfeI AflIII AhdI AleI
AlwNI ApoI AvaI BaeI BanI BanII BbsI BcgI
BciVI BglI BglII BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspHI BspMI BsrDI BsrFI BsrGI BstAPI
BstXI BstYI BtgI BtsI CspCI DrdI EaeI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII
Hin4I HincII HpaI MmeI MscI MslI MspA1I NcoI
NdeI NspI PciI PflMI PfoI PpiI PsiI PstI
PvuII SacI SapI SfcI SmlI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AflII AgeI AlfI AloI ApaI ApaLI
AscI AseI AsiSI AvrII BamHI BbeI BbvCI BclI
BfrBI BlpI Bme1580I BmgBI BmtI BsaAI BsaBI BsiWI
BsmI BsmBI BspEI BsrBI BssHII BssSI BstBI BstEII
BstZ17I Bsu36I BtgZI ClaI DraI DraIII EagI EcoRV
FseI FspI FspAI HindIII KasI KpnI MfeI MluI
NaeI NarI NgoMIV NheI NotI NruI NsiI PacI
PasI PmeI PmlI PpuMI PshAI PspOMI PspXI PsrI
PvuI RsrII SacII SalI SanDI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI SwaI XhoI XmaI ZraI