Restriction map for
pFN21AE3762
(Linear) (Six-base) MAPSORT of: KIEE3762.seq Check: 3599 from: 1 to: 1701
KIEE3762.orf
With 182 enzymes: SgfI *
March 22, 2012 16:43 ..
Acc65I G'GTAC_C
Cuts at: 0 145 1701
Size: 145 1556
AclI AA'CG_TT
Cuts at: 0 900 1701
Size: 900 801
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 472 532 1040 1701
Size: 472 60 508 661
Fragments arranged by size:
661 508 472 60
AleI CACnn'nnGTG
Cuts at: 0 785 1701
Size: 785 916
AlwNI CAG_nnn'CTG
Cuts at: 0 213 376 654 1671 1701
Size: 213 163 278 1017 30
Fragments arranged by size:
1017 278 213 163 30
ApaI G_GGCC'C
Cuts at: 0 463 721 1069 1701
Size: 463 258 348 632
Fragments arranged by size:
632 463 348 258
ApoI r'AATT_y
Cuts at: 0 691 1701
Size: 691 1010
AvaI C'yCGr_G
Cuts at: 0 470 1189 1610 1701
Size: 470 719 421 91
Fragments arranged by size:
719 470 421 91
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 129 162 1701
Size: 129 33 1539
Fragments arranged by size:
1539 129 33
BanI G'GyrC_C
Cuts at: 0 31 145 445 1701
Size: 31 114 300 1256
Fragments arranged by size:
1256 300 114 31
BanII G_rGCy'C
Cuts at: 0 369 463 721 1022 1069 1124 1196 1218
Size: 369 94 258 301 47 55 72 22
Cuts at: 1218 1701
Size: 483
Fragments arranged by size:
483 369 301 258 94 72 55 47
22
BbsI GAAGACnn'nnnn_
Cuts at: 0 1510 1701
Size: 1510 191
BbvCI CC'TCA_GC
Cuts at: 0 1170 1701
Size: 1170 531
BglI GCCn_nnn'nGGC
Cuts at: 0 15 1701
Size: 15 1686
BlpI GC'TnA_GC
Cuts at: 0 19 1530 1701
Size: 19 1511 171
Fragments arranged by size:
1511 171 19
Bme1580I G_kGCm'C
Cuts at: 0 394 450 463 721 1069 1233 1467 1701
Size: 394 56 13 258 348 164 234 234
Fragments arranged by size:
394 348 258 234 234 164 56 13
BmgBI CAC'GTC
Cuts at: 0 1087 1701
Size: 1087 614
BmrI ACTGGGnnnn_n'
Cuts at: 0 534 642 1583 1701
Size: 534 108 941 118
Fragments arranged by size:
941 534 118 108
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 277 1701
Size: 277 1424
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 272 353 398 1049 1701
Size: 272 81 45 651 652
Fragments arranged by size:
652 651 272 81 45
Bpu10I CC'TnA_GC
Cuts at: 0 1170 1701
Size: 1170 531
BsaI GGTCTCn'nnnn_
Cuts at: 0 994 1086 1701
Size: 994 92 615
Fragments arranged by size:
994 615 92
BsaBI GATnn'nnATC
Cuts at: 0 818 1701
Size: 818 883
BsaWI w'CCGG_w
Cuts at: 0 731 1343 1701
Size: 731 612 358
Fragments arranged by size:
731 612 358
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 27 253 658 904 1143 1146 1168 1595
Size: 27 226 405 246 239 3 22 427
Cuts at: 1595 1700 1701
Size: 105 1
Fragments arranged by size:
427 405 246 239 226 105 27 22
3 1
BseYI C'CCAG_C
Cuts at: 0 51 480 749 1158 1261 1330 1701
Size: 51 429 269 409 103 69 371
Fragments arranged by size:
429 409 371 269 103 69 51
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 627 1684 1701
Size: 627 1057 17
Fragments arranged by size:
1057 627 17
BsiHKAI G_wGCw'C
Cuts at: 0 1041 1701
Size: 1041 660
BsmI GAATG_Cn'
Cuts at: 0 703 1701
Size: 703 998
Bsp1286I G_dGCh'C
Cuts at: 0 369 394 450 463 721 1022 1041 1069
Size: 369 25 56 13 258 301 19 28
Cuts at: 1069 1124 1196 1218 1233 1467 1701
Size: 55 72 22 15 234 234
Fragments arranged by size:
369 301 258 234 234 72 56 55
28 25 22 19 15 13
BspEI T'CCGG_A
Cuts at: 0 731 1343 1701
Size: 731 612 358
Fragments arranged by size:
731 612 358
BsrBI CCG'CTC
Cuts at: 0 26 1212 1701
Size: 26 1186 489
Fragments arranged by size:
1186 489 26
BsrFI r'CCGG_y
Cuts at: 0 305 1701
Size: 305 1396
BstEII G'GTnAC_C
Cuts at: 0 1415 1600 1701
Size: 1415 185 101
Fragments arranged by size:
1415 185 101
BstYI r'GATC_y
Cuts at: 0 827 1701
Size: 827 874
Bsu36I CC'TnA_GG
Cuts at: 0 1126 1701
Size: 1126 575
BtsI GCAGTG_nn'
Cuts at: 0 613 631 1181 1231 1701
Size: 613 18 550 50 470
Fragments arranged by size:
613 550 470 50 18
DraIII CAC_nnn'GTG
Cuts at: 0 650 1701
Size: 650 1051
EaeI y'GGCC_r
Cuts at: 0 113 1701
Size: 113 1588
EarI CTCTTCn'nnn_
Cuts at: 0 964 1701
Size: 964 737
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 272 353 398 472 532 1040 1049 1701
Size: 272 81 45 74 60 508 9 652
Fragments arranged by size:
652 508 272 81 74 60 45 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 454 1073 1701
Size: 454 619 628
Fragments arranged by size:
628 619 454
EcoO109I rG'GnC_Cy
Cuts at: 0 48 459 460 717 718 1065 1066 1385
Size: 48 411 1 257 1 347 1 319
Cuts at: 1385 1617 1629 1701
Size: 232 12 72
Fragments arranged by size:
411 347 319 257 232 72 48 12
1 1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 825 1701
Size: 825 876
FspI TGC'GCA
Cuts at: 0 1180 1701
Size: 1180 521
HaeII r_GCGC'y
Cuts at: 0 1104 1701
Size: 1104 597
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 379 411 1679 1701
Size: 379 32 1268 22
Fragments arranged by size:
1268 379 32 22
HindIII A'AGCT_T
Cuts at: 0 490 1701
Size: 490 1211
KpnI G_GTAC'C
Cuts at: 0 149 1701
Size: 149 1552
MscI TGG'CCA
Cuts at: 0 115 1701
Size: 115 1586
MslI CAynn'nnrTG
Cuts at: 0 785 1345 1701
Size: 785 560 356
Fragments arranged by size:
785 560 356
MspA1I CmG'CkG
Cuts at: 0 317 1292 1322 1701
Size: 317 975 30 379
Fragments arranged by size:
975 379 317 30
NdeI CA'TA_TG
Cuts at: 0 504 1701
Size: 504 1197
PasI CC'CwG_GG
Cuts at: 0 298 362 580 1069 1092 1701
Size: 298 64 218 489 23 609
Fragments arranged by size:
609 489 298 218 64 23
PflMI CCAn_nnn'nTGG
Cuts at: 0 376 1701
Size: 376 1325
PpuMI rG'GwC_Cy
Cuts at: 0 48 1385 1617 1629 1701
Size: 48 1337 232 12 72
Fragments arranged by size:
1337 232 72 48 12
PshAI GACnn'nnGTC
Cuts at: 0 292 1701
Size: 292 1409
PspOMI G'GGCC_C
Cuts at: 0 459 717 1065 1701
Size: 459 258 348 636
Fragments arranged by size:
636 459 348 258
PstI C_TGCA'G
Cuts at: 0 608 626 1226 1238 1701
Size: 608 18 600 12 463
Fragments arranged by size:
608 600 463 18 12
PvuII CAG'CTG
Cuts at: 0 317 1701
Size: 317 1384
SanDI GG'GwC_CC
Cuts at: 0 48 1617 1701
Size: 48 1569 84
Fragments arranged by size:
1569 84 48
SexAI A'CCwGG_T
Cuts at: 0 1670 1701
Size: 1670 31
SfcI C'TryA_G
Cuts at: 0 604 622 739 1222 1234 1701
Size: 604 18 117 483 12 467
Fragments arranged by size:
604 483 467 117 18 12
SmaI CCC'GGG
Cuts at: 0 472 1701
Size: 472 1229
SrfI GCCC'GGGC
Cuts at: 0 472 1701
Size: 472 1229
StuI AGG'CCT
Cuts at: 0 84 640 1240 1701
Size: 84 556 600 461
Fragments arranged by size:
600 556 461 84
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 661 741 1701
Size: 661 80 960
Fragments arranged by size:
960 661 80
Tth111I GACn'n_nGTC
Cuts at: 0 1621 1701
Size: 1621 80
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 156 1701
Size: 156 1545
XmaI C'CCGG_G
Cuts at: 0 470 1701
Size: 470 1231
Enzymes that do cut:
Acc65I AclI AcuI AleI AlwNI ApaI ApoI AvaI
BaeI BanI BanII BbsI BbvCI BglI BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BsaI BsaBI BsaWI
BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI BsrBI
BsrFI BstEII BstYI Bsu36I BtsI DraIII EaeI EarI
Eco57MI EcoNI EcoO109I FalI FspI HaeII Hin4I HindIII
KpnI MscI MslI MspA1I NdeI PasI PflMI PpuMI
PshAI PspOMI PstI PvuII SanDI SexAI SfcI SmaI
SrfI StuI TaqII Tth111I XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI AfeI AflII AflIII AgeI AhdI
AlfI AloI ApaLI AscI AseI AsiSI AvrII BamHI
BbeI BcgI BciVI BclI BfrBI BglII BmtI BpuEI
BsaAI BsaHI BsaXI BsiEI BsiWI BsmBI BspHI BspMI
BsrDI BsrGI BssHII BssSI BstAPI BstBI BstXI BstZ17I
BtgI BtgZI ClaI CspCI DraI DrdI EagI EciI
EcoICRI EcoRI EcoRV FseI FspAI HincII HpaI KasI
MfeI MluI MmeI NaeI NarI NcoI NgoMIV NheI
NotI NruI NsiI NspI PacI PciI PfoI PmeI
PmlI PpiI PsiI PspXI PsrI PvuI RsrII SacI
SacII SalI SapI SbfI ScaI SfiI SfoI SgfI
SgrAI SmlI SnaBI SpeI SphI SspI StyI SwaI
TatI TstI XbaI XhoI XmnI ZraI