Restriction map for
pFN21AE3761
(Linear) (Six-base) MAPSORT of: KIEE3761.seq Check: 9173 from: 1 to: 1656
KIEE3761.orf
With 182 enzymes: SgfI *
March 22, 2012 16:43 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 566 599 854 1271 1562 1656
Size: 566 33 255 417 291 94
Fragments arranged by size:
566 417 291 255 94 33
AlwNI CAG_nnn'CTG
Cuts at: 0 369 544 577 1339 1656
Size: 369 175 33 762 317
Fragments arranged by size:
762 369 317 175 33
ApoI r'AATT_y
Cuts at: 0 191 502 895 1656
Size: 191 311 393 761
Fragments arranged by size:
761 393 311 191
BanI G'GyrC_C
Cuts at: 0 73 628 1656
Size: 73 555 1028
Fragments arranged by size:
1028 555 73
BbsI GAAGACnn'nnnn_
Cuts at: 0 842 1568 1656
Size: 842 726 88
Fragments arranged by size:
842 726 88
BbvCI CC'TCA_GC
Cuts at: 0 1457 1656
Size: 1457 199
BclI T'GATC_A
Cuts at: 0 245 1656
Size: 245 1411
BfrBI ATG'CAT
Cuts at: 0 738 1071 1303 1656
Size: 738 333 232 353
Fragments arranged by size:
738 353 333 232
BglII A'GATC_T
Cuts at: 0 1438 1656
Size: 1438 218
BmrI ACTGGGnnnn_n'
Cuts at: 0 812 1656
Size: 812 844
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 311 612 1656
Size: 311 301 1044
Fragments arranged by size:
1044 311 301
Bpu10I CC'TnA_GC
Cuts at: 0 1457 1656
Size: 1457 199
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 932 1656
Size: 932 724
BsaI GGTCTCn'nnnn_
Cuts at: 0 407 1656
Size: 407 1249
BsaAI yAC'GTr
Cuts at: 0 48 1350 1656
Size: 48 1302 306
Fragments arranged by size:
1302 306 48
BsaHI Gr'CG_yC
Cuts at: 0 68 1656
Size: 68 1588
BsaWI w'CCGG_w
Cuts at: 0 1561 1656
Size: 1561 95
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 585 615 1656
Size: 585 30 1041
Fragments arranged by size:
1041 585 30
BseYI C'CCAG_C
Cuts at: 0 51 63 1656
Size: 51 12 1593
Fragments arranged by size:
1593 51 12
BsmI GAATG_Cn'
Cuts at: 0 459 1305 1656
Size: 459 846 351
Fragments arranged by size:
846 459 351
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 562 738 1656
Size: 562 176 918
Fragments arranged by size:
918 562 176
BsrGI T'GTAC_A
Cuts at: 0 904 1656
Size: 904 752
BstAPI GCAn_nnn'nTGC
Cuts at: 0 744 1656
Size: 744 912
BstEII G'GTnAC_C
Cuts at: 0 940 1656
Size: 940 716
BstYI r'GATC_y
Cuts at: 0 523 674 1438 1656
Size: 523 151 764 218
Fragments arranged by size:
764 523 218 151
BtgI C'CryG_G
Cuts at: 0 1607 1656
Size: 1607 49
BtsI GCAGTG_nn'
Cuts at: 0 968 1656
Size: 968 688
DraI TTT'AAA
Cuts at: 0 1407 1656
Size: 1407 249
DraIII CAC_nnn'GTG
Cuts at: 0 48 1656
Size: 48 1608
EarI CTCTTCn'nnn_
Cuts at: 0 329 623 1656
Size: 329 294 1033
Fragments arranged by size:
1033 329 294
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 311 566 599 612 854 1271 1562 1656
Size: 311 255 33 13 242 417 291 94
Fragments arranged by size:
417 311 291 255 242 94 33 13
EcoO109I rG'GnC_Cy
Cuts at: 0 574 1656
Size: 574 1082
EcoRV GAT'ATC
Cuts at: 0 588 1656
Size: 588 1068
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 973 1656
Size: 973 683
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 237 269 671 703 758 790 1098 1130
Size: 237 32 402 32 55 32 308 32
Cuts at: 1130 1423 1455 1656
Size: 293 32 201
Fragments arranged by size:
402 308 293 237 201 55 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 753 1656
Size: 753 903
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 153 280 419 1656
Size: 153 127 139 1237
Fragments arranged by size:
1237 153 139 127
MslI CAynn'nnrTG
Cuts at: 0 452 1313 1656
Size: 452 861 343
Fragments arranged by size:
861 452 343
MspA1I CmG'CkG
Cuts at: 0 1275 1656
Size: 1275 381
NcoI C'CATG_G
Cuts at: 0 1607 1656
Size: 1607 49
NdeI CA'TA_TG
Cuts at: 0 398 740 1656
Size: 398 342 916
Fragments arranged by size:
916 398 342
NsiI A_TGCA'T
Cuts at: 0 740 1073 1305 1656
Size: 740 333 232 351
Fragments arranged by size:
740 351 333 232
NspI r_CATG'y
Cuts at: 0 655 1656
Size: 655 1001
PflMI CCAn_nnn'nTGG
Cuts at: 0 123 1339 1656
Size: 123 1216 317
Fragments arranged by size:
1216 317 123
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1428 1460 1656
Size: 1428 32 196
Fragments arranged by size:
1428 196 32
PpuMI rG'GwC_Cy
Cuts at: 0 574 1656
Size: 574 1082
PstI C_TGCA'G
Cuts at: 0 749 1521 1656
Size: 749 772 135
Fragments arranged by size:
772 749 135
PvuII CAG'CTG
Cuts at: 0 1275 1656
Size: 1275 381
SapI GCTCTTCn'nnn_
Cuts at: 0 623 1656
Size: 623 1033
SexAI A'CCwGG_T
Cuts at: 0 239 1373 1656
Size: 239 1134 283
Fragments arranged by size:
1134 283 239
SfcI C'TryA_G
Cuts at: 0 745 1517 1656
Size: 745 772 139
Fragments arranged by size:
772 745 139
SmlI C'TyrA_G
Cuts at: 0 947 1656
Size: 947 709
SpeI A'CTAG_T
Cuts at: 0 514 1656
Size: 514 1142
StuI AGG'CCT
Cuts at: 0 1085 1656
Size: 1085 571
StyI C'CwwG_G
Cuts at: 0 1607 1656
Size: 1607 49
TatI w'GTAC_w
Cuts at: 0 904 1656
Size: 904 752
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 32 64 585 617 1656
Size: 32 32 521 32 1039
Fragments arranged by size:
1039 521 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1534 1656
Size: 1534 122
Enzymes that do cut:
AcuI AlwNI ApoI BanI BbsI BbvCI BclI BfrBI
BglII BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI
BsaWI BsaXI BseYI BsmI BspMI BsrGI BstAPI BstEII
BstYI BtgI BtsI DraI DraIII EarI Eco57MI EcoO109I
EcoRV FalI Hin4I HincII MmeI MslI MspA1I NcoI
NdeI NsiI NspI PflMI PpiI PpuMI PstI PvuII
SapI SexAI SfcI SmlI SpeI StuI StyI TatI
TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BanII BbeI
BcgI BciVI BglI BlpI Bme1580I BmgBI BmtI BplI
BsaBI BseRI BsgI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I
BspEI BspHI BsrBI BsrDI BsrFI BssHII BssSI BstBI
BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DrdI EaeI
EagI EciI EcoICRI EcoNI EcoRI FseI FspI FspAI
HaeII HindIII HpaI KasI KpnI MfeI MluI MscI
NaeI NarI NgoMIV NheI NotI NruI PacI PasI
PciI PfoI PmeI PmlI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI SbfI
ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SphI
SrfI SspI SwaI TaqII Tth111I XbaI XhoI XmaI
XmnI ZraI