Restriction map for
pFN21AE3759
(Linear) (Six-base) MAPSORT of: KIEE3759.seq Check: 7786 from: 1 to: 1443
KIEE3759.orf
With 182 enzymes: SgfI *
March 22, 2012 16:43 ..
Acc65I G'GTAC_C
Cuts at: 0 554 1443
Size: 554 889
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 266 1145 1443
Size: 266 879 298
Fragments arranged by size:
879 298 266
AgeI A'CCGG_T
Cuts at: 0 23 1443
Size: 23 1420
ApaI G_GGCC'C
Cuts at: 0 108 1443
Size: 108 1335
ApoI r'AATT_y
Cuts at: 0 313 359 814 1003 1137 1443
Size: 313 46 455 189 134 306
Fragments arranged by size:
455 313 306 189 134 46
AvaI C'yCGr_G
Cuts at: 0 202 1443
Size: 202 1241
BamHI G'GATC_C
Cuts at: 0 6 411 1443
Size: 6 405 1032
Fragments arranged by size:
1032 405 6
BanI G'GyrC_C
Cuts at: 0 121 554 571 1443
Size: 121 433 17 872
Fragments arranged by size:
872 433 121 17
BanII G_rGCy'C
Cuts at: 0 20 52 108 1443
Size: 20 32 56 1335
Fragments arranged by size:
1335 56 32 20
BbeI G_GCGC'C
Cuts at: 0 125 1443
Size: 125 1318
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 553 587 1443
Size: 553 34 856
Fragments arranged by size:
856 553 34
BfrBI ATG'CAT
Cuts at: 0 295 1443
Size: 295 1148
BglII A'GATC_T
Cuts at: 0 1191 1271 1443
Size: 1191 80 172
Fragments arranged by size:
1191 172 80
Bme1580I G_kGCm'C
Cuts at: 0 108 1443
Size: 108 1335
BmgBI CAC'GTC
Cuts at: 0 210 1443
Size: 210 1233
BmrI ACTGGGnnnn_n'
Cuts at: 0 1098 1443
Size: 1098 345
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 27 1443
Size: 27 1416
Bpu10I CC'TnA_GC
Cuts at: 0 808 1387 1443
Size: 808 579 56
Fragments arranged by size:
808 579 56
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 365 1088 1443
Size: 365 723 355
Fragments arranged by size:
723 365 355
BsaI GGTCTCn'nnnn_
Cuts at: 0 222 1443
Size: 222 1221
BsaHI Gr'CG_yC
Cuts at: 0 31 122 1443
Size: 31 91 1321
Fragments arranged by size:
1321 91 31
BsaWI w'CCGG_w
Cuts at: 0 23 245 1443
Size: 23 222 1198
Fragments arranged by size:
1198 222 23
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 70 73 76 178 199 1443
Size: 70 3 3 102 21 1244
Fragments arranged by size:
1244 102 70 21 3 3
BsiEI CG_ry'CG
Cuts at: 0 24 1443
Size: 24 1419
BsmI GAATG_Cn'
Cuts at: 0 295 297 1443
Size: 295 2 1146
Fragments arranged by size:
1146 295 2
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1292 1443
Size: 1292 151
Bsp1286I G_dGCh'C
Cuts at: 0 20 52 108 1443
Size: 20 32 56 1335
Fragments arranged by size:
1335 56 32 20
BsrBI CCG'CTC
Cuts at: 0 75 1443
Size: 75 1368
BsrDI GCAATG_nn'
Cuts at: 0 924 1443
Size: 924 519
BsrFI r'CCGG_y
Cuts at: 0 23 175 1443
Size: 23 152 1268
Fragments arranged by size:
1268 152 23
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1088 1443
Size: 1088 355
BstYI r'GATC_y
Cuts at: 0 6 411 1191 1271 1443
Size: 6 405 780 80 172
Fragments arranged by size:
780 405 172 80 6
BtgI C'CryG_G
Cuts at: 0 35 91 112 257 1443
Size: 35 56 21 145 1186
Fragments arranged by size:
1186 145 56 35 21
DraI TTT'AAA
Cuts at: 0 1053 1443
Size: 1053 390
DrdI GACnn_nn'nnGTC
Cuts at: 0 214 1443
Size: 214 1229
EaeI y'GGCC_r
Cuts at: 0 525 1443
Size: 525 918
EarI CTCTTCn'nnn_
Cuts at: 0 449 1300 1443
Size: 449 851 143
Fragments arranged by size:
851 449 143
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 266 1145 1443
Size: 266 879 298
Fragments arranged by size:
879 298 266
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1285 1443
Size: 1285 158
EcoRI G'AATT_C
Cuts at: 0 1003 1443
Size: 1003 440
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1024 1443
Size: 1024 419
HaeII r_GCGC'y
Cuts at: 0 125 137 1443
Size: 125 12 1306
Fragments arranged by size:
1306 125 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 531 563 1062 1094 1251 1283 1443
Size: 531 32 499 32 157 32 160
Fragments arranged by size:
531 499 160 157 32 32 32
HincII GTy'rAC
Cuts at: 0 469 1443
Size: 469 974
HindIII A'AGCT_T
Cuts at: 0 592 1443
Size: 592 851
KasI G'GCGC_C
Cuts at: 0 121 1443
Size: 121 1322
KpnI G_GTAC'C
Cuts at: 0 558 1443
Size: 558 885
MfeI C'AATT_G
Cuts at: 0 1025 1413 1443
Size: 1025 388 30
Fragments arranged by size:
1025 388 30
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 336 1354 1443
Size: 336 1018 89
Fragments arranged by size:
1018 336 89
MslI CAynn'nnrTG
Cuts at: 0 1061 1443
Size: 1061 382
MspA1I CmG'CkG
Cuts at: 0 37 84 93 114 259 1443
Size: 37 47 9 21 145 1184
Fragments arranged by size:
1184 145 47 37 21 9
NaeI GCC'GGC
Cuts at: 0 177 1443
Size: 177 1266
NarI GG'CG_CC
Cuts at: 0 122 1443
Size: 122 1321
NgoMIV G'CCGG_C
Cuts at: 0 175 1443
Size: 175 1268
NsiI A_TGCA'T
Cuts at: 0 297 1443
Size: 297 1146
PfoI T'CCnGG_A
Cuts at: 0 220 1443
Size: 220 1223
PspOMI G'GGCC_C
Cuts at: 0 104 1443
Size: 104 1339
SacII CC_GC'GG
Cuts at: 0 38 94 115 260 1443
Size: 38 56 21 145 1183
Fragments arranged by size:
1183 145 56 38 21
SapI GCTCTTCn'nnn_
Cuts at: 0 449 1443
Size: 449 994
SfoI GGC'GCC
Cuts at: 0 123 1443
Size: 123 1320
SmaI CCC'GGG
Cuts at: 0 204 1443
Size: 204 1239
SmlI C'TyrA_G
Cuts at: 0 380 1103 1443
Size: 380 723 340
Fragments arranged by size:
723 380 340
SspI AAT'ATT
Cuts at: 0 802 843 1443
Size: 802 41 600
Fragments arranged by size:
802 600 41
TatI w'GTAC_w
Cuts at: 0 1323 1443
Size: 1323 120
XmaI C'CCGG_G
Cuts at: 0 202 1443
Size: 202 1241
XmnI GAAnn'nnTTC
Cuts at: 0 295 1443
Size: 295 1148
Enzymes that do cut:
Acc65I AcuI AgeI ApaI ApoI AvaI BamHI BanI
BanII BbeI BcgI BfrBI BglII Bme1580I BmgBI BmrI
BplI Bpu10I BpuEI BsaI BsaHI BsaWI BseRI BsiEI
BsmI BsmBI Bsp1286I BsrBI BsrDI BsrFI BstXI BstYI
BtgI DraI DrdI EaeI EarI Eco57MI EcoNI EcoRI
FalI HaeII Hin4I HincII HindIII KasI KpnI MfeI
MmeI MslI MspA1I NaeI NarI NgoMIV NsiI PfoI
PspOMI SacII SapI SfoI SmaI SmlI SspI TatI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AhdI
AleI AlfI AloI AlwNI ApaLI AscI AseI AsiSI
AvrII BaeI BbsI BbvCI BciVI BclI BglI BlpI
BmtI BpmI BsaAI BsaBI BsaXI BseYI BsgI BsiHKAI
BsiWI BspEI BspHI BspMI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstZ17I Bsu36I BtgZI BtsI ClaI CspCI
DraIII EagI EciI EcoICRI EcoO109I EcoRV FseI FspI
FspAI HpaI MluI MscI NcoI NdeI NheI NotI
NruI NspI PacI PasI PciI PflMI PmeI PmlI
PpiI PpuMI PshAI PsiI PspXI PsrI PstI PvuI
PvuII RsrII SacI SalI SanDI SbfI ScaI SexAI
SfcI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI
StuI StyI SwaI TaqII TstI Tth111I XbaI XcmI
XhoI ZraI