Restriction map for
pFN21AE3757
(Linear) (Six-base) MAPSORT of: KIEE3757.seq Check: 8839 from: 1 to: 1185
KIEE3757.orf
With 182 enzymes: SgfI *
March 22, 2012 16:43 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 352 1185
Size: 352 833
AatII G_ACGT'C
Cuts at: 0 731 1185
Size: 731 454
AccI GT'mk_AC
Cuts at: 0 731 1185
Size: 731 454
Acc65I G'GTAC_C
Cuts at: 0 524 1185
Size: 524 661
AfeI AGC'GCT
Cuts at: 0 32 287 1185
Size: 32 255 898
Fragments arranged by size:
898 255 32
AleI CACnn'nnGTG
Cuts at: 0 877 1185
Size: 877 308
AlwNI CAG_nnn'CTG
Cuts at: 0 245 1185
Size: 245 940
ApaLI G'TGCA_C
Cuts at: 0 430 1185
Size: 430 755
ApoI r'AATT_y
Cuts at: 0 1122 1185
Size: 1122 63
AvaI C'yCGr_G
Cuts at: 0 70 196 791 1185
Size: 70 126 595 394
Fragments arranged by size:
595 394 126 70
BanI G'GyrC_C
Cuts at: 0 116 524 1185
Size: 116 408 661
Fragments arranged by size:
661 408 116
BanII G_rGCy'C
Cuts at: 0 44 275 283 339 1185
Size: 44 231 8 56 846
Fragments arranged by size:
846 231 56 44 8
BbeI G_GCGC'C
Cuts at: 0 120 1185
Size: 120 1065
BciVI GTATCCnnnnn_n'
Cuts at: 0 915 1185
Size: 915 270
BclI T'GATC_A
Cuts at: 0 981 1185
Size: 981 204
BglI GCCn_nnn'nGGC
Cuts at: 0 163 172 1185
Size: 163 9 1013
Fragments arranged by size:
1013 163 9
BlpI GC'TnA_GC
Cuts at: 0 282 934 1185
Size: 282 652 251
Fragments arranged by size:
652 282 251
Bme1580I G_kGCm'C
Cuts at: 0 205 434 1185
Size: 205 229 751
Fragments arranged by size:
751 229 205
BmrI ACTGGGnnnn_n'
Cuts at: 0 869 1185
Size: 869 316
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 856 1185
Size: 856 329
Bpu10I CC'TnA_GC
Cuts at: 0 1035 1185
Size: 1035 150
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 879 1185
Size: 879 306
BsaI GGTCTCn'nnnn_
Cuts at: 0 658 1185
Size: 658 527
BsaAI yAC'GTr
Cuts at: 0 144 1185
Size: 144 1041
BsaHI Gr'CG_yC
Cuts at: 0 117 575 728 1185
Size: 117 458 153 457
Fragments arranged by size:
458 457 153 117
BsaWI w'CCGG_w
Cuts at: 0 388 1185
Size: 388 797
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 694 724 934 964 1185
Size: 694 30 210 30 221
Fragments arranged by size:
694 221 210 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1063 1185
Size: 1063 122
BseYI C'CCAG_C
Cuts at: 0 322 365 1185
Size: 322 43 820
Fragments arranged by size:
820 322 43
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 244 700 1185
Size: 244 456 485
Fragments arranged by size:
485 456 244
BsiEI CG_ry'CG
Cuts at: 0 8 305 585 1185
Size: 8 297 280 600
Fragments arranged by size:
600 297 280 8
BsiHKAI G_wGCw'C
Cuts at: 0 44 275 434 842 1185
Size: 44 231 159 408 343
Fragments arranged by size:
408 343 231 159 44
Bsp1286I G_dGCh'C
Cuts at: 0 44 205 275 283 339 434 842 1185
Size: 44 161 70 8 56 95 408 343
Fragments arranged by size:
408 343 161 95 70 56 44 8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 352 1185
Size: 352 833
BsrBI CCG'CTC
Cuts at: 0 23 1185
Size: 23 1162
BsrDI GCAATG_nn'
Cuts at: 0 824 1185
Size: 824 361
BsrFI r'CCGG_y
Cuts at: 0 104 252 577 1185
Size: 104 148 325 608
Fragments arranged by size:
608 325 148 104
BssHII G'CGCG_C
Cuts at: 0 479 1185
Size: 479 706
BstAPI GCAn_nnn'nTGC
Cuts at: 0 83 1185
Size: 83 1102
BstXI CCAn_nnnn'nTGG
Cuts at: 0 393 1185
Size: 393 792
Bsu36I CC'TnA_GG
Cuts at: 0 1046 1185
Size: 1046 139
BtgI C'CryG_G
Cuts at: 0 1070 1185
Size: 1070 115
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 295 1185
Size: 295 890
BtsI GCAGTG_nn'
Cuts at: 0 809 1185
Size: 809 376
EaeI y'GGCC_r
Cuts at: 0 5 99 138 164 302 612 912 1185
Size: 5 94 39 26 138 310 300 273
Fragments arranged by size:
310 300 273 138 94 39 26 5
EagI C'GGCC_G
Cuts at: 0 5 302 1185
Size: 5 297 883
Fragments arranged by size:
883 297 5
EarI CTCTTCn'nnn_
Cuts at: 0 1133 1185
Size: 1133 52
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 70 1185
Size: 70 1115
EcoICRI GAG'CTC
Cuts at: 0 42 273 1185
Size: 42 231 912
Fragments arranged by size:
912 231 42
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 856 1185
Size: 856 329
EcoO109I rG'GnC_Cy
Cuts at: 0 371 710 747 1185
Size: 371 339 37 438
Fragments arranged by size:
438 371 339 37
EcoRI G'AATT_C
Cuts at: 0 1122 1185
Size: 1122 63
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 102 766 1185
Size: 102 664 419
Fragments arranged by size:
664 419 102
HaeII r_GCGC'y
Cuts at: 0 34 120 289 329 400 1185
Size: 34 86 169 40 71 785
Fragments arranged by size:
785 169 86 71 40 34
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 657 689 694 726 966 998 1132 1164
Size: 657 32 5 32 240 32 134 32
Cuts at: 1164 1185
Size: 21
Fragments arranged by size:
657 240 134 32 32 32 32 21
5
HincII GTy'rAC
Cuts at: 0 381 732 1176 1185
Size: 381 351 444 9
Fragments arranged by size:
444 381 351 9
HindIII A'AGCT_T
Cuts at: 0 622 1185
Size: 622 563
KasI G'GCGC_C
Cuts at: 0 116 1185
Size: 116 1069
KpnI G_GTAC'C
Cuts at: 0 528 1185
Size: 528 657
MscI TGG'CCA
Cuts at: 0 101 614 1185
Size: 101 513 571
Fragments arranged by size:
571 513 101
MslI CAynn'nnrTG
Cuts at: 0 595 877 1185
Size: 595 282 308
Fragments arranged by size:
595 308 282
MspA1I CmG'CkG
Cuts at: 0 114 970 1185
Size: 114 856 215
Fragments arranged by size:
856 215 114
NaeI GCC'GGC
Cuts at: 0 579 1185
Size: 579 606
NarI GG'CG_CC
Cuts at: 0 117 1185
Size: 117 1068
NcoI C'CATG_G
Cuts at: 0 1070 1185
Size: 1070 115
NgoMIV G'CCGG_C
Cuts at: 0 577 1185
Size: 577 608
PasI CC'CwG_GG
Cuts at: 0 852 1185
Size: 852 333
PfoI T'CCnGG_A
Cuts at: 0 722 833 1185
Size: 722 111 352
Fragments arranged by size:
722 352 111
PmlI CAC'GTG
Cuts at: 0 144 1185
Size: 144 1041
PpuMI rG'GwC_Cy
Cuts at: 0 371 710 747 1185
Size: 371 339 37 438
Fragments arranged by size:
438 371 339 37
PshAI GACnn'nnGTC
Cuts at: 0 376 1185
Size: 376 809
PspXI vC'TCGA_Gb
Cuts at: 0 70 196 1185
Size: 70 126 989
Fragments arranged by size:
989 126 70
PstI C_TGCA'G
Cuts at: 0 214 1185
Size: 214 971
PvuI CG_AT'CG
Cuts at: 0 585 1185
Size: 585 600
SacI G_AGCT'C
Cuts at: 0 44 275 1185
Size: 44 231 910
Fragments arranged by size:
910 231 44
SalI G'TCGA_C
Cuts at: 0 730 1185
Size: 730 455
SanDI GG'GwC_CC
Cuts at: 0 371 710 1185
Size: 371 339 475
Fragments arranged by size:
475 371 339
SfcI C'TryA_G
Cuts at: 0 210 1185
Size: 210 975
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 163 1185
Size: 163 1022
SfoI GGC'GCC
Cuts at: 0 118 1185
Size: 118 1067
SgrAI Cr'CCGG_yG
Cuts at: 0 577 1185
Size: 577 608
SmlI C'TyrA_G
Cuts at: 0 70 196 894 1185
Size: 70 126 698 291
Fragments arranged by size:
698 291 126 70
StyI C'CwwG_G
Cuts at: 0 652 1070 1115 1185
Size: 652 418 45 70
Fragments arranged by size:
652 418 70 45
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 831 1185
Size: 831 354
XhoI C'TCGA_G
Cuts at: 0 70 196 1185
Size: 70 126 989
Fragments arranged by size:
989 126 70
ZraI GAC'GTC
Cuts at: 0 729 1185
Size: 729 456
Enzymes that do cut:
AarI AatII AccI Acc65I AfeI AleI AlwNI ApaLI
ApoI AvaI BanI BanII BbeI BciVI BclI BglI
BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaAI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspMI BsrBI BsrDI BsrFI BssHII BstAPI BstXI
Bsu36I BtgI BtgZI BtsI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI HaeII Hin4I HincII
HindIII KasI KpnI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV PasI PfoI PmlI PpuMI PshAI PspXI
PstI PvuI SacI SalI SanDI SfcI SfiI SfoI
SgrAI SmlI StyI XcmI XhoI ZraI
Enzymes that do not cut:
AclI AcuI AflII AflIII AgeI AhdI AlfI AloI
ApaI AscI AseI AsiSI AvrII BaeI BamHI BbsI
BbvCI BcgI BfrBI BglII BmgBI BmtI BplI BsaBI
BsiWI BsmI BsmBI BspEI BspHI BsrGI BssSI BstBI
BstEII BstYI BstZ17I ClaI CspCI DraI DraIII DrdI
EcoNI EcoRV FseI FspI FspAI HpaI MfeI MluI
MmeI NdeI NheI NotI NruI NsiI NspI PacI
PciI PflMI PmeI PpiI PsiI PspOMI PsrI PvuII
RsrII SacII SapI SbfI ScaI SexAI SgfI SmaI
SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII
TatI TstI Tth111I XbaI XmaI XmnI