Restriction map for
pFN21AE3756
(Linear) (Six-base) MAPSORT of: KIEE3756.seq Check: 4594 from: 1 to: 1116
KIEE3756.orf
With 182 enzymes: SgfI *
July 3, 2012 18:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 401 1116
Size: 401 715
AccI GT'mk_AC
Cuts at: 0 83 623 767 1116
Size: 83 540 144 349
Fragments arranged by size:
540 349 144 83
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 519 1116
Size: 519 597
AflIII A'CryG_T
Cuts at: 0 179 311 1116
Size: 179 132 805
Fragments arranged by size:
805 179 132
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 580 937 1116
Size: 580 357 179
Fragments arranged by size:
580 357 179
AlwNI CAG_nnn'CTG
Cuts at: 0 1050 1116
Size: 1050 66
ApaLI G'TGCA_C
Cuts at: 0 157 370 475 1116
Size: 157 213 105 641
Fragments arranged by size:
641 213 157 105
AscI GG'CGCG_CC
Cuts at: 0 655 1116
Size: 655 461
AvaI C'yCGr_G
Cuts at: 0 508 1116
Size: 508 608
BanI G'GyrC_C
Cuts at: 0 292 859 1116
Size: 292 567 257
Fragments arranged by size:
567 292 257
BanII G_rGCy'C
Cuts at: 0 110 1116
Size: 110 1006
BbeI G_GCGC'C
Cuts at: 0 296 863 1116
Size: 296 567 253
Fragments arranged by size:
567 296 253
BbsI GAAGACnn'nnnn_
Cuts at: 0 129 633 697 1073 1116
Size: 129 504 64 376 43
Fragments arranged by size:
504 376 129 64 43
Bme1580I G_kGCm'C
Cuts at: 0 161 374 464 479 746 937 1116
Size: 161 213 90 15 267 191 179
Fragments arranged by size:
267 213 191 179 161 90 15
BmtI G_CTAG'C
Cuts at: 0 127 1116
Size: 127 989
BsaBI GATnn'nnATC
Cuts at: 0 340 1116
Size: 340 776
BsaHI Gr'CG_yC
Cuts at: 0 293 665 860 1019 1116
Size: 293 372 195 159 97
Fragments arranged by size:
372 293 195 159 97
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 6 1116
Size: 6 1110
BseYI C'CCAG_C
Cuts at: 0 591 1116
Size: 591 525
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 55 235 1116
Size: 55 180 881
Fragments arranged by size:
881 180 55
BsiEI CG_ry'CG
Cuts at: 0 819 1116
Size: 819 297
BsiHKAI G_wGCw'C
Cuts at: 0 161 374 479 1116
Size: 161 213 105 637
Fragments arranged by size:
637 213 161 105
BsmBI CGTCTCn'nnnn_
Cuts at: 0 238 1116
Size: 238 878
Bsp1286I G_dGCh'C
Cuts at: 0 110 161 374 464 479 746 937 1116
Size: 110 51 213 90 15 267 191 179
Fragments arranged by size:
267 213 191 179 110 90 51 15
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 212 401 1116
Size: 212 189 715
Fragments arranged by size:
715 212 189
BsrFI r'CCGG_y
Cuts at: 0 856 1116
Size: 856 260
BsrGI T'GTAC_A
Cuts at: 0 176 1116
Size: 176 940
BssHII G'CGCG_C
Cuts at: 0 144 146 655 1116
Size: 144 2 509 461
Fragments arranged by size:
509 461 144 2
BssSI C'ACGA_G
Cuts at: 0 571 1116
Size: 571 545
BstAPI GCAn_nnn'nTGC
Cuts at: 0 378 1116
Size: 378 738
BtsI GCAGTG_nn'
Cuts at: 0 247 1116
Size: 247 869
DraIII CAC_nnn'GTG
Cuts at: 0 78 165 1094 1116
Size: 78 87 929 22
Fragments arranged by size:
929 87 78 22
EaeI y'GGCC_r
Cuts at: 0 194 581 797 854 959 1116
Size: 194 387 216 57 105 157
Fragments arranged by size:
387 216 194 157 105 57
EarI CTCTTCn'nnn_
Cuts at: 0 214 1116
Size: 214 902
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 702 993 1098 1116
Size: 702 291 105 18
Fragments arranged by size:
702 291 105 18
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 519 1116
Size: 519 597
EcoO109I rG'GnC_Cy
Cuts at: 0 482 504 953 1116
Size: 482 22 449 163
Fragments arranged by size:
482 449 163 22
HaeII r_GCGC'y
Cuts at: 0 296 442 592 863 902 985 1116
Size: 296 146 150 271 39 83 131
Fragments arranged by size:
296 271 150 146 131 83 39
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1057 1089 1116
Size: 1057 32 27
Fragments arranged by size:
1057 32 27
KasI G'GCGC_C
Cuts at: 0 292 859 1116
Size: 292 567 257
Fragments arranged by size:
567 292 257
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1080 1088 1116
Size: 1080 8 28
Fragments arranged by size:
1080 28 8
MslI CAynn'nnrTG
Cuts at: 0 346 448 1116
Size: 346 102 668
Fragments arranged by size:
668 346 102
MspA1I CmG'CkG
Cuts at: 0 323 378 681 882 1116
Size: 323 55 303 201 234
Fragments arranged by size:
323 303 234 201 55
NaeI GCC'GGC
Cuts at: 0 858 1116
Size: 858 258
NarI GG'CG_CC
Cuts at: 0 293 860 1116
Size: 293 567 256
Fragments arranged by size:
567 293 256
NgoMIV G'CCGG_C
Cuts at: 0 856 1116
Size: 856 260
NheI G'CTAG_C
Cuts at: 0 123 1116
Size: 123 993
NspI r_CATG'y
Cuts at: 0 183 315 1116
Size: 183 132 801
Fragments arranged by size:
801 183 132
PciI A'CATG_T
Cuts at: 0 179 311 1116
Size: 179 132 805
Fragments arranged by size:
805 179 132
PpuMI rG'GwC_Cy
Cuts at: 0 504 953 1116
Size: 504 449 163
Fragments arranged by size:
504 449 163
PspXI vC'TCGA_Gb
Cuts at: 0 508 1116
Size: 508 608
PvuI CG_AT'CG
Cuts at: 0 819 1116
Size: 819 297
PvuII CAG'CTG
Cuts at: 0 323 1116
Size: 323 793
SapI GCTCTTCn'nnn_
Cuts at: 0 214 1116
Size: 214 902
SfcI C'TryA_G
Cuts at: 0 84 1116
Size: 84 1032
SfoI GGC'GCC
Cuts at: 0 294 861 1116
Size: 294 567 255
Fragments arranged by size:
567 294 255
SmlI C'TyrA_G
Cuts at: 0 508 1116
Size: 508 608
TatI w'GTAC_w
Cuts at: 0 176 887 1116
Size: 176 711 229
Fragments arranged by size:
711 229 176
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1080 1112 1116
Size: 1080 32 4
Fragments arranged by size:
1080 32 4
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 870 1116
Size: 870 246
XhoI C'TCGA_G
Cuts at: 0 508 1116
Size: 508 608
Enzymes that do cut:
AarI AccI AcuI AflIII AlfI AlwNI ApaLI AscI
AvaI BanI BanII BbeI BbsI Bme1580I BmtI BsaBI
BsaHI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspMI BsrFI BsrGI BssHII BssSI BstAPI BtsI DraIII
EaeI EarI EciI Eco57MI EcoO109I HaeII Hin4I KasI
MmeI MslI MspA1I NaeI NarI NgoMIV NheI NspI
PciI PpuMI PspXI PvuI PvuII SapI SfcI SfoI
SmlI TatI TstI XcmI XhoI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AleI
AloI ApaI ApoI AseI AsiSI AvrII BaeI BamHI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaWI BsaXI BsiWI BsmI BspEI BspHI BsrBI BsrDI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
ClaI CspCI DraI DrdI EagI EcoICRI EcoNI EcoRI
EcoRV FalI FseI FspI FspAI HincII HindIII HpaI
KpnI MfeI MluI MscI NcoI NdeI NotI NruI
NsiI PacI PasI PflMI PfoI PmeI PmlI PpiI
PshAI PsiI PspOMI PsrI PstI RsrII SacI SacII
SalI SanDI SbfI ScaI SexAI SfiI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI StyI
SwaI TaqII Tth111I XbaI XmaI XmnI ZraI