(Linear) (Six-base) MAPSORT of: KIEE3755.seq  Check: 4212  from: 1  to: 1083



KIEE3755.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 16:42  ..

AccI GT'mk_AC

Cuts at:      0     488    1083 
   Size:        488     595
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     593    1083 
   Size:        593     490
 
AlwNI CAG_nnn'CTG

Cuts at:      0     372    1083 
   Size:        372     711
 
ApoI r'AATT_y

Cuts at:      0     133    1083 
   Size:        133     950
 
BamHI G'GATC_C

Cuts at:      0     554    1083 
   Size:        554     529
 
BanI G'GyrC_C

Cuts at:      0     757    1083 
   Size:        757     326
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      32     941     976    1083 
   Size:         32     909      35     107

  Fragments arranged by size: 

                 909     107      35      32
 
BglII A'GATC_T

Cuts at:      0     228    1083 
   Size:        228     855
 
Bme1580I G_kGCm'C

Cuts at:      0     760    1083 
   Size:        760     323
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     281     449     994    1083 
   Size:        281     168     545      89

  Fragments arranged by size: 

                 545     281     168      89
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1052    1083 
   Size:       1052      31
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     472     712    1083 
   Size:        472     240     371

  Fragments arranged by size: 

                 472     371     240
 
Bpu10I CC'TnA_GC

Cuts at:      0    1027    1083 
   Size:       1027      56
 
BsaWI w'CCGG_w

Cuts at:      0      71     462    1083 
   Size:         71     391     621

  Fragments arranged by size: 

                 621     391      71
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     641    1083 
   Size:        641     442
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     134     526    1083 
   Size:        134     392     557

  Fragments arranged by size: 

                 557     392     134
 
BsiEI CG_ry'CG

Cuts at:      0     111    1083 
   Size:        111     972
 
Bsp1286I G_dGCh'C

Cuts at:      0     760    1083 
   Size:        760     323
 
BspEI T'CCGG_A

Cuts at:      0      71     462    1083 
   Size:         71     391     621

  Fragments arranged by size: 

                 621     391      71
 
BstYI r'GATC_y

Cuts at:      0     228     554    1044    1083 
   Size:        228     326     490      39

  Fragments arranged by size: 

                 490     326     228      39
 
BstZ17I GTA'TAC

Cuts at:      0     489    1083 
   Size:        489     594
 
BtgI C'CryG_G

Cuts at:      0     287     324    1083 
   Size:        287      37     759

  Fragments arranged by size: 

                 759     287      37
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     729    1083 
   Size:        729     354
 
BtsI GCAGTG_nn'

Cuts at:      0     270    1083 
   Size:        270     813
 
ClaI AT'CG_AT

Cuts at:      0     107    1083 
   Size:        107     976
 
DraI TTT'AAA

Cuts at:      0     138    1083 
   Size:        138     945
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     886    1083 
   Size:        886     197
 
EarI CTCTTCn'nnn_

Cuts at:      0     515     569     938     959    1083 
   Size:        515      54     369      21     124

  Fragments arranged by size: 

                 515     369     124      54      21
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     472     593     712    1083 
   Size:        472     121     119     371

  Fragments arranged by size: 

                 472     371     121     119
 
EcoO109I rG'GnC_Cy

Cuts at:      0     307     604    1023    1083 
   Size:        307     297     419      60

  Fragments arranged by size: 

                 419     307     297      60
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     432    1083 
   Size:        432     651
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     762     794    1083 
   Size:        762      32     289

  Fragments arranged by size: 

                 762     289      32
 
HincII GTy'rAC

Cuts at:      0     584    1083 
   Size:        584     499
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     880     932    1083 
   Size:        880      52     151

  Fragments arranged by size: 

                 880     151      52
 
MslI CAynn'nnrTG

Cuts at:      0     344    1083 
   Size:        344     739
 
MspA1I CmG'CkG

Cuts at:      0     929    1083 
   Size:        929     154
 
NcoI C'CATG_G

Cuts at:      0     287     324    1083 
   Size:        287      37     759

  Fragments arranged by size: 

                 759     287      37
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     330     735    1083 
   Size:        330     405     348

  Fragments arranged by size: 

                 405     348     330
 
PfoI T'CCnGG_A

Cuts at:      0     728    1083 
   Size:        728     355
 
PpuMI rG'GwC_Cy

Cuts at:      0     307     604    1023    1083 
   Size:        307     297     419      60

  Fragments arranged by size: 

                 419     307     297      60
 
PsiI TTA'TAA

Cuts at:      0     152    1083 
   Size:        152     931
 
PstI C_TGCA'G

Cuts at:      0     236    1083 
   Size:        236     847
 
PvuI CG_AT'CG

Cuts at:      0     111    1083 
   Size:        111     972
 
PvuII CAG'CTG

Cuts at:      0     929    1083 
   Size:        929     154
 
SfcI C'TryA_G

Cuts at:      0     232    1083 
   Size:        232     851
 
StyI C'CwwG_G

Cuts at:      0     287     324    1083 
   Size:        287      37     759

  Fragments arranged by size: 

                 759     287      37
 
TatI w'GTAC_w

Cuts at:      0     524    1083 
   Size:        524     559
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      53      85    1083 
   Size:         53      32     998

  Fragments arranged by size: 

                 998      53      32
 

 Enzymes that do cut:

     AccI     AcuI    AlwNI     ApoI    BamHI     BanI     BbsI    BglII
 Bme1580I     BmrI     BplI     BpmI   Bpu10I    BsaWI    BseRI     BsgI
    BsiEI Bsp1286I    BspEI    BstYI  BstZ17I     BtgI    BtgZI     BtsI
     ClaI     DraI     DrdI     EarI  Eco57MI EcoO109I     FalI    Hin4I
   HincII     MmeI     MslI   MspA1I     NcoI    PflMI     PfoI    PpuMI
     PsiI     PstI     PvuI    PvuII     SfcI     StyI     TatI     TstI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BanII     BbeI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BglI     BlpI    BmgBI     BmtI    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaXI    BseYI  BsiHKAI    BsiWI
     BsmI    BsmBI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI   Bsu36I    CspCI
   DraIII     EaeI     EagI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV
     FseI     FspI    FspAI    HaeII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MscI     NaeI     NarI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI     PmeI
     PmlI     PpiI    PshAI   PspOMI    PspXI     PsrI    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI    TaqII  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI