Restriction map for
pFN21AE3754
(Linear) (Six-base) MAPSORT of: KIEE3754.seq Check: 6774 from: 1 to: 747
KIEE3754.orf
With 182 enzymes: SgfI *
March 22, 2012 16:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 361 747
Size: 361 386
AccI GT'mk_AC
Cuts at: 0 524 747
Size: 524 223
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 550 747
Size: 550 197
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 73 747
Size: 73 674
AlwNI CAG_nnn'CTG
Cuts at: 0 592 747
Size: 592 155
ApaI G_GGCC'C
Cuts at: 0 614 696 747
Size: 614 82 51
Fragments arranged by size:
614 82 51
ApaLI G'TGCA_C
Cuts at: 0 281 499 747
Size: 281 218 248
Fragments arranged by size:
281 248 218
ApoI r'AATT_y
Cuts at: 0 580 747
Size: 580 167
AvaI C'yCGr_G
Cuts at: 0 467 607 747
Size: 467 140 140
Fragments arranged by size:
467 140 140
BamHI G'GATC_C
Cuts at: 0 237 674 747
Size: 237 437 73
Fragments arranged by size:
437 237 73
BanI G'GyrC_C
Cuts at: 0 32 49 747
Size: 32 17 698
Fragments arranged by size:
698 32 17
BanII G_rGCy'C
Cuts at: 0 309 479 614 696 747
Size: 309 170 135 82 51
Fragments arranged by size:
309 170 135 82 51
BbeI G_GCGC'C
Cuts at: 0 36 747
Size: 36 711
BglI GCCn_nnn'nGGC
Cuts at: 0 595 747
Size: 595 152
Bme1580I G_kGCm'C
Cuts at: 0 52 285 503 614 696 747
Size: 52 233 218 111 82 51
Fragments arranged by size:
233 218 111 82 52 51
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 600 747
Size: 600 147
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 405 595 747
Size: 405 190 152
Fragments arranged by size:
405 190 152
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 685 747
Size: 685 62
BsaHI Gr'CG_yC
Cuts at: 0 33 747
Size: 33 714
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 187 202 747
Size: 187 15 545
Fragments arranged by size:
545 187 15
BseYI C'CCAG_C
Cuts at: 0 75 395 472 614 747
Size: 75 320 77 142 133
Fragments arranged by size:
320 142 133 77 75
BsiHKAI G_wGCw'C
Cuts at: 0 285 503 747
Size: 285 218 244
Fragments arranged by size:
285 244 218
Bsp1286I G_dGCh'C
Cuts at: 0 52 285 309 479 503 614 696 747
Size: 52 233 24 170 24 111 82 51
Fragments arranged by size:
233 170 111 82 52 51 24 24
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 361 747
Size: 361 386
BsrBI CCG'CTC
Cuts at: 0 69 747
Size: 69 678
BstXI CCAn_nnnn'nTGG
Cuts at: 0 637 747
Size: 637 110
BstYI r'GATC_y
Cuts at: 0 237 674 747
Size: 237 437 73
Fragments arranged by size:
437 237 73
BtgI C'CryG_G
Cuts at: 0 119 747
Size: 119 628
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 128 747
Size: 128 619
BtsI GCAGTG_nn'
Cuts at: 0 590 747
Size: 590 157
EaeI y'GGCC_r
Cuts at: 0 2 116 655 747
Size: 2 114 539 92
Fragments arranged by size:
539 114 92 2
EarI CTCTTCn'nnn_
Cuts at: 0 306 739 747
Size: 306 433 8
Fragments arranged by size:
433 306 8
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 39 141 610 747
Size: 39 102 469 137
Fragments arranged by size:
469 137 102 39
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 405 550 595 747
Size: 405 145 45 152
Fragments arranged by size:
405 152 145 45
EcoNI CCTnn'n_nnAGG
Cuts at: 0 628 747
Size: 628 119
EcoO109I rG'GnC_Cy
Cuts at: 0 198 611 662 747
Size: 198 413 51 85
Fragments arranged by size:
413 198 85 51
EcoRI G'AATT_C
Cuts at: 0 580 747
Size: 580 167
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 555 747
Size: 555 192
HaeII r_GCGC'y
Cuts at: 0 36 94 747
Size: 36 58 653
Fragments arranged by size:
653 58 36
KasI G'GCGC_C
Cuts at: 0 32 747
Size: 32 715
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 185 747
Size: 185 562
MslI CAynn'nnrTG
Cuts at: 0 506 747
Size: 506 241
NarI GG'CG_CC
Cuts at: 0 33 747
Size: 33 714
PasI CC'CwG_GG
Cuts at: 0 689 747
Size: 689 58
PflMI CCAn_nnn'nTGG
Cuts at: 0 349 747
Size: 349 398
PpuMI rG'GwC_Cy
Cuts at: 0 198 662 747
Size: 198 464 85
Fragments arranged by size:
464 198 85
PspOMI G'GGCC_C
Cuts at: 0 610 692 747
Size: 610 82 55
Fragments arranged by size:
610 82 55
SapI GCTCTTCn'nnn_
Cuts at: 0 306 747
Size: 306 441
SfoI GGC'GCC
Cuts at: 0 34 747
Size: 34 713
SmaI CCC'GGG
Cuts at: 0 469 747
Size: 469 278
SmlI C'TyrA_G
Cuts at: 0 700 747
Size: 700 47
SpeI A'CTAG_T
Cuts at: 0 261 747
Size: 261 486
TatI w'GTAC_w
Cuts at: 0 488 747
Size: 488 259
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 375 407 747
Size: 375 32 340
Fragments arranged by size:
375 340 32
XmaI C'CCGG_G
Cuts at: 0 467 747
Size: 467 280
Enzymes that do cut:
AarI AccI AcuI AlfI AlwNI ApaI ApaLI ApoI
AvaI BamHI BanI BanII BbeI BglI Bme1580I BplI
BpmI BpuEI BsaHI BseRI BseYI BsiHKAI Bsp1286I BspMI
BsrBI BstXI BstYI BtgI BtgZI BtsI EaeI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII KasI
MmeI MslI NarI PasI PflMI PpuMI PspOMI SapI
SfoI SmaI SmlI SpeI TatI TstI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AloI AscI AseI AsiSI AvrII BaeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglII BlpI BmgBI
BmrI BmtI Bpu10I BsaI BsaAI BsaBI BsaWI BsaXI
BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I
Bsu36I ClaI CspCI DraI DraIII DrdI EagI EcoICRI
EcoRV FseI FspI FspAI Hin4I HincII HindIII HpaI
KpnI MfeI MluI MscI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PciI
PfoI PmeI PmlI PpiI PshAI PsiI PspXI PsrI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SnaBI
SphI SrfI SspI StuI StyI SwaI TaqII Tth111I
XbaI XcmI XhoI XmnI ZraI