(Linear) (Six-base) MAPSORT of: KIEE3753.seq  Check: 5562  from: 1  to: 603



KIEE3753.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 16:42  ..

AlwNI CAG_nnn'CTG

Cuts at:      0     289     603 
   Size:        289     314
 
ApoI r'AATT_y

Cuts at:      0     176     603 
   Size:        176     427
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     459     603 
   Size:        459     144
 
BsaBI GATnn'nnATC

Cuts at:      0      62     603 
   Size:         62     541
 
BsaWI w'CCGG_w

Cuts at:      0     566     603 
   Size:        566      37
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     203     460     603 
   Size:        203     257     143

  Fragments arranged by size: 

                 257     203     143
 
BstEII G'GTnAC_C

Cuts at:      0     360     603 
   Size:        360     243
 
BstYI r'GATC_y

Cuts at:      0     286     603 
   Size:        286     317
 
Bsu36I CC'TnA_GG

Cuts at:      0     356     603 
   Size:        356     247
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     513     603 
   Size:        513      90
 
EaeI y'GGCC_r

Cuts at:      0     572     603 
   Size:        572      31
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     459     603 
   Size:        459     144
 
EcoO109I rG'GnC_Cy

Cuts at:      0     277     603 
   Size:        277     326
 
EcoRI G'AATT_C

Cuts at:      0     176     603 
   Size:        176     427
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     381     603 
   Size:        381     222
 
HincII GTy'rAC

Cuts at:      0     161     266     603 
   Size:        161     105     337

  Fragments arranged by size: 

                 337     161     105
 
MscI TGG'CCA

Cuts at:      0     574     603 
   Size:        574      29
 
MspA1I CmG'CkG

Cuts at:      0     148     603 
   Size:        148     455
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     581     603 
   Size:        581      22
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      89     121     603 
   Size:         89      32     482

  Fragments arranged by size: 

                 482      89      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     277     603 
   Size:        277     326
 
SanDI GG'GwC_CC

Cuts at:      0     277     603 
   Size:        277     326
 
SfcI C'TryA_G

Cuts at:      0     222     603 
   Size:        222     381
 
SpeI A'CTAG_T

Cuts at:      0      40     163     603 
   Size:         40     123     440

  Fragments arranged by size: 

                 440     123      40
 
SspI AAT'ATT

Cuts at:      0     393     603 
   Size:        393     210
 
StyI C'CwwG_G

Cuts at:      0     402     603 
   Size:        402     201
 
TatI w'GTAC_w

Cuts at:      0     539     603 
   Size:        539      64
 
XmnI GAAnn'nnTTC

Cuts at:      0      77     104     603 
   Size:         77      27     499

  Fragments arranged by size: 

                 499      77      27
 

 Enzymes that do cut:

    AlwNI     ApoI     BpmI    BsaBI    BsaWI    BseRI   BstEII    BstYI
   Bsu36I    BtgZI     EaeI  Eco57MI EcoO109I    EcoRI     FalI   HincII
     MscI   MspA1I    PflMI     PpiI    PpuMI    SanDI     SfcI     SpeI
     SspI     StyI     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI    BsaXI    BseYI     BsgI
    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI    BstXI  BstZ17I     BtgI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EagI     EarI     EciI  EcoICRI    EcoNI    EcoRV
     FseI     FspI    FspAI    HaeII    Hin4I  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MslI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI     PfoI     PmeI     PmlI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SphI     SrfI     StuI     SwaI
    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI