(Linear) (Six-base) MAPSORT of: KIEE3752.seq  Check: 462  from: 1  to: 474



KIEE3752.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 16:42  ..

AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     356     388     474 
   Size:        356      32      86

  Fragments arranged by size: 

                 356      86      32
 
ApoI r'AATT_y

Cuts at:      0     226     474 
   Size:        226     248
 
BbvCI CC'TCA_GC

Cuts at:      0      16     474 
   Size:         16     458
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     129     474 
   Size:        129     345
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     327     474 
   Size:        327     147
 
Bpu10I CC'TnA_GC

Cuts at:      0      16     474 
   Size:         16     458
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     371     474 
   Size:        371     103
 
BsaBI GATnn'nnATC

Cuts at:      0     200     474 
   Size:        200     274
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     307     474 
   Size:        307     167
 
BseYI C'CCAG_C

Cuts at:      0     381     474 
   Size:        381      93
 
BsiHKAI G_wGCw'C

Cuts at:      0     450     474 
   Size:        450      24
 
Bsp1286I G_dGCh'C

Cuts at:      0     450     474 
   Size:        450      24
 
BspHI T'CATG_A

Cuts at:      0     113     474 
   Size:        113     361
 
BstBI TT'CG_AA

Cuts at:      0     230     474 
   Size:        230     244
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      64     474 
   Size:         64     410
 
BstYI r'GATC_y

Cuts at:      0     332     474 
   Size:        332     142
 
BtgI C'CryG_G

Cuts at:      0     154     474 
   Size:        154     320
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     327     474 
   Size:        327     147
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     190     222     474 
   Size:        190      32     252

  Fragments arranged by size: 

                 252     190      32
 
MslI CAynn'nnrTG

Cuts at:      0     219     251     474 
   Size:        219      32     223

  Fragments arranged by size: 

                 223     219      32
 
PfoI T'CCnGG_A

Cuts at:      0     135     474 
   Size:        135     339
 
SmlI C'TyrA_G

Cuts at:      0     386     474 
   Size:        386      88
 
StuI AGG'CCT

Cuts at:      0     397     474 
   Size:        397      77
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      74     474 
   Size:         74     400
 

 Enzymes that do cut:

     AloI     ApoI    BbvCI     BmrI     BpmI   Bpu10I    BpuEI    BsaBI
    BseRI    BseYI  BsiHKAI Bsp1286I    BspHI    BstBI    BstXI    BstYI
     BtgI  Eco57MI    Hin4I     MslI     PfoI     SmlI     StuI    TaqII

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BbsI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmtI     BplI     BsaI    BsaAI
    BsaHI    BsaWI    BsaXI     BsgI    BsiEI    BsiWI     BsmI    BsmBI
    BspEI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI   BstEII  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI
    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SspI     StyI     SwaI     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI