Restriction map for
pFN21AE3721
(Linear) (Six-base) MAPSORT of: KIEE3721.seq Check: 4413 from: 1 to: 1239
KIEE3721.orf
With 182 enzymes: SgfI *
July 3, 2012 18:17 ..
AccI GT'mk_AC
Cuts at: 0 266 1239
Size: 266 973
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 276 1239
Size: 276 963
AfeI AGC'GCT
Cuts at: 0 373 1239
Size: 373 866
AflIII A'CryG_T
Cuts at: 0 824 1239
Size: 824 415
AleI CACnn'nnGTG
Cuts at: 0 448 1239
Size: 448 791
AlwNI CAG_nnn'CTG
Cuts at: 0 606 712 1239
Size: 606 106 527
Fragments arranged by size:
606 527 106
AseI AT'TA_AT
Cuts at: 0 566 1239
Size: 566 673
BanI G'GyrC_C
Cuts at: 0 248 1239
Size: 248 991
BclI T'GATC_A
Cuts at: 0 107 1239
Size: 107 1132
BfrBI ATG'CAT
Cuts at: 0 720 1239
Size: 720 519
Bme1580I G_kGCm'C
Cuts at: 0 251 1239
Size: 251 988
BmgBI CAC'GTC
Cuts at: 0 996 1239
Size: 996 243
BmrI ACTGGGnnnn_n'
Cuts at: 0 700 762 1239
Size: 700 62 477
Fragments arranged by size:
700 477 62
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 103 388 812 1239
Size: 70 33 285 424 427
Fragments arranged by size:
427 424 285 70 33
BsaBI GATnn'nnATC
Cuts at: 0 349 1239
Size: 349 890
BsaWI w'CCGG_w
Cuts at: 0 507 1239
Size: 507 732
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 147 1239
Size: 147 1092
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 514 559 1239
Size: 514 45 680
Fragments arranged by size:
680 514 45
BsiEI CG_ry'CG
Cuts at: 0 464 1239
Size: 464 775
BsiHKAI G_wGCw'C
Cuts at: 0 212 1239
Size: 212 1027
BsmI GAATG_Cn'
Cuts at: 0 60 1239
Size: 60 1179
Bsp1286I G_dGCh'C
Cuts at: 0 212 251 1239
Size: 212 39 988
Fragments arranged by size:
988 212 39
BsrDI GCAATG_nn'
Cuts at: 0 785 1239
Size: 785 454
BsrFI r'CCGG_y
Cuts at: 0 1220 1239
Size: 1220 19
BsrGI T'GTAC_A
Cuts at: 0 842 1239
Size: 842 397
BssSI C'ACGA_G
Cuts at: 0 1020 1239
Size: 1020 219
BstEII G'GTnAC_C
Cuts at: 0 20 1239
Size: 20 1219
BstXI CCAn_nnnn'nTGG
Cuts at: 0 33 244 1239
Size: 33 211 995
Fragments arranged by size:
995 211 33
BstYI r'GATC_y
Cuts at: 0 350 1239
Size: 350 889
Bsu36I CC'TnA_GG
Cuts at: 0 485 1239
Size: 485 754
BtgI C'CryG_G
Cuts at: 0 386 1239
Size: 386 853
BtsI GCAGTG_nn'
Cuts at: 0 547 1239
Size: 547 692
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 810 845 1239
Size: 810 35 394
Fragments arranged by size:
810 394 35
EaeI y'GGCC_r
Cuts at: 0 234 849 1049 1094 1183 1239
Size: 234 615 200 45 89 56
Fragments arranged by size:
615 234 200 89 56 45
EarI CTCTTCn'nnn_
Cuts at: 0 1033 1239
Size: 1033 206
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 103 276 388 812 1239
Size: 70 33 173 112 424 427
Fragments arranged by size:
427 424 173 112 70 33
HaeII r_GCGC'y
Cuts at: 0 375 1239
Size: 375 864
HindIII A'AGCT_T
Cuts at: 0 739 1073 1239
Size: 739 334 166
Fragments arranged by size:
739 334 166
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 930 1239
Size: 930 309
MscI TGG'CCA
Cuts at: 0 1051 1096 1185 1239
Size: 1051 45 89 54
Fragments arranged by size:
1051 89 54 45
MslI CAynn'nnrTG
Cuts at: 0 242 448 451 859 1239
Size: 242 206 3 408 380
Fragments arranged by size:
408 380 242 206 3
MspA1I CmG'CkG
Cuts at: 0 174 848 1239
Size: 174 674 391
Fragments arranged by size:
674 391 174
NaeI GCC'GGC
Cuts at: 0 1222 1239
Size: 1222 17
NcoI C'CATG_G
Cuts at: 0 386 1239
Size: 386 853
NgoMIV G'CCGG_C
Cuts at: 0 1220 1239
Size: 1220 19
NsiI A_TGCA'T
Cuts at: 0 722 1239
Size: 722 517
NspI r_CATG'y
Cuts at: 0 828 1239
Size: 828 411
PciI A'CATG_T
Cuts at: 0 824 1239
Size: 824 415
PflMI CCAn_nnn'nTGG
Cuts at: 0 392 712 1103 1239
Size: 392 320 391 136
Fragments arranged by size:
392 391 320 136
PfoI T'CCnGG_A
Cuts at: 0 179 1239
Size: 179 1060
ScaI AGT'ACT
Cuts at: 0 1126 1239
Size: 1126 113
SexAI A'CCwGG_T
Cuts at: 0 711 1239
Size: 711 528
StyI C'CwwG_G
Cuts at: 0 386 1143 1239
Size: 386 757 96
Fragments arranged by size:
757 386 96
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 39 450 1239
Size: 39 411 789
Fragments arranged by size:
789 411 39
TatI w'GTAC_w
Cuts at: 0 842 1124 1239
Size: 842 282 115
Fragments arranged by size:
842 282 115
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 363 1239
Size: 363 876
XmnI GAAnn'nnTTC
Cuts at: 0 742 1239
Size: 742 497
Enzymes that do cut:
AccI AcuI AfeI AflIII AleI AlwNI AseI BanI
BclI BfrBI Bme1580I BmgBI BmrI BpmI BsaBI BsaWI
BseRI BsgI BsiEI BsiHKAI BsmI Bsp1286I BsrDI BsrFI
BsrGI BssSI BstEII BstXI BstYI Bsu36I BtgI BtsI
CspCI EaeI EarI Eco57MI HaeII HindIII MmeI MscI
MslI MspA1I NaeI NcoI NgoMIV NsiI NspI PciI
PflMI PfoI ScaI SexAI StyI TaqII TatI XcmI
XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AgeI AhdI AlfI
AloI ApaI ApaLI ApoI AscI AsiSI AvaI AvrII
BaeI BamHI BanII BbeI BbsI BbvCI BcgI BciVI
BglI BglII BlpI BmtI BplI Bpu10I BpuEI BsaI
BsaAI BsaHI BsaXI BseYI BsiWI BsmBI BspEI BspHI
BspMI BsrBI BssHII BstAPI BstBI BstZ17I BtgZI ClaI
DraI DraIII DrdI EagI EciI EcoICRI EcoNI EcoO109I
EcoRI EcoRV FalI FseI FspI FspAI Hin4I HincII
HpaI KasI KpnI MfeI MluI NarI NdeI NheI
NotI NruI PacI PasI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI SwaI TstI Tth111I XbaI
XhoI XmaI ZraI