(Linear) (Six-base) MAPSORT of: KIEE3709.seq  Check: 4701  from: 1  to: 636



KIEE3709.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 16:41  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      73     379     636 
   Size:         73     306     257

  Fragments arranged by size: 

                 306     257      73
 
AfeI AGC'GCT

Cuts at:      0     384     636 
   Size:        384     252
 
AflIII A'CryG_T

Cuts at:      0     494     534     636 
   Size:        494      40     102

  Fragments arranged by size: 

                 494     102      40
 
AleI CACnn'nnGTG

Cuts at:      0     449     636 
   Size:        449     187
 
BclI T'GATC_A

Cuts at:      0     248     636 
   Size:        248     388
 
BfrBI ATG'CAT

Cuts at:      0      26     636 
   Size:         26     610
 
BglI GCCn_nnn'nGGC

Cuts at:      0      79     636 
   Size:         79     557
 
BglII A'GATC_T

Cuts at:      0     303     521     636 
   Size:        303     218     115

  Fragments arranged by size: 

                 303     218     115
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     198     636 
   Size:        198     438
 
Bpu10I CC'TnA_GC

Cuts at:      0     206     636 
   Size:        206     430
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     397     636 
   Size:        397     239
 
BsaAI yAC'GTr

Cuts at:      0     497     636 
   Size:        497     139
 
BsaHI Gr'CG_yC

Cuts at:      0     145     636 
   Size:        145     491
 
BsaWI w'CCGG_w

Cuts at:      0     150     636 
   Size:        150     486
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     337     367     636 
   Size:        337      30     269

  Fragments arranged by size: 

                 337     269      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     277     358     636 
   Size:        277      81     278

  Fragments arranged by size: 

                 278     277      81
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     278     636 
   Size:        278     358
 
BsmI GAATG_Cn'

Cuts at:      0     225     636 
   Size:        225     411
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     136     636 
   Size:        136     500
 
BsrBI CCG'CTC

Cuts at:      0     162     636 
   Size:        162     474
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     451     636 
   Size:        451     185
 
BstYI r'GATC_y

Cuts at:      0     218     303     521     636 
   Size:        218      85     218     115

  Fragments arranged by size: 

                 218     218     115      85
 
BtgI C'CryG_G

Cuts at:      0     135     636 
   Size:        135     501
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      73     379     636 
   Size:         73     306     257

  Fragments arranged by size: 

                 306     257      73
 
EcoO109I rG'GnC_Cy

Cuts at:      0      93     126     636 
   Size:         93      33     510

  Fragments arranged by size: 

                 510      93      33
 
HaeII r_GCGC'y

Cuts at:      0     386     636 
   Size:        386     250
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     115     147     236     268     418     450     636 
   Size:        115      32      89      32     150      32     186

  Fragments arranged by size: 

                 186     150     115      89      32      32      32
 
MslI CAynn'nnrTG

Cuts at:      0     449     636 
   Size:        449     187
 
NsiI A_TGCA'T

Cuts at:      0      28     636 
   Size:         28     608
 
NspI r_CATG'y

Cuts at:      0      26     538     636 
   Size:         26     512      98

  Fragments arranged by size: 

                 512      98      26
 
PasI CC'CwG_GG

Cuts at:      0     620     636 
   Size:        620      16
 
PciI A'CATG_T

Cuts at:      0     534     636 
   Size:        534     102
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     626     636 
   Size:        626      10
 
PpuMI rG'GwC_Cy

Cuts at:      0      93     636 
   Size:         93     543
 
SmlI C'TyrA_G

Cuts at:      0     412     636 
   Size:        412     224
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     337     369     636 
   Size:        337      32     267

  Fragments arranged by size: 

                 337     267      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     627     636 
   Size:        627       9
 

 Enzymes that do cut:

     AcuI     AfeI   AflIII     AleI     BclI    BfrBI     BglI    BglII
     BmrI   Bpu10I    BpuEI    BsaAI    BsaHI    BsaWI    BsaXI    BseRI
     BsgI     BsmI    BsmBI    BsrBI    BstXI    BstYI     BtgI  Eco57MI
 EcoO109I    HaeII    Hin4I     MslI     NsiI     NspI     PasI     PciI
    PflMI    PpuMI     SmlI     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI    AflII     AgeI     AhdI
     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI     BlpI Bme1580I    BmgBI     BmtI
     BplI     BpmI     BsaI    BsaBI    BseYI    BsiEI  BsiHKAI    BsiWI
 Bsp1286I    BspEI    BspHI    BspMI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     PacI     PfoI     PmeI     PmlI     PpiI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII
     TatI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI