Restriction map for
pFN21AE3704
(Linear) (Six-base) MAPSORT of: KIEE3704.seq Check: 8395 from: 1 to: 3429
KIEE3704.orf
With 182 enzymes: SgfI *
July 3, 2012 18:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2142 3429
Size: 2142 1287
AccI GT'mk_AC
Cuts at: 0 779 2285 3429
Size: 779 1506 1144
Fragments arranged by size:
1506 1144 779
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 181 803 1052 1279 2421 2479 2629 2889
Size: 181 622 249 227 1142 58 150 260
Cuts at: 2889 3429
Size: 540
Fragments arranged by size:
1142 622 540 260 249 227 181 150
58
AfeI AGC'GCT
Cuts at: 0 1615 3429
Size: 1615 1814
AleI CACnn'nnGTG
Cuts at: 0 1627 3004 3014 3429
Size: 1627 1377 10 415
Fragments arranged by size:
1627 1377 415 10
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 853 885 3429
Size: 853 32 2544
Fragments arranged by size:
2544 853 32
AlwNI CAG_nnn'CTG
Cuts at: 0 3319 3429
Size: 3319 110
ApaLI G'TGCA_C
Cuts at: 0 3007 3429
Size: 3007 422
ApoI r'AATT_y
Cuts at: 0 165 791 2570 3423 3429
Size: 165 626 1779 853 6
Fragments arranged by size:
1779 853 626 165 6
AseI AT'TA_AT
Cuts at: 0 650 3272 3429
Size: 650 2622 157
Fragments arranged by size:
2622 650 157
BamHI G'GATC_C
Cuts at: 0 1083 1187 3429
Size: 1083 104 2242
Fragments arranged by size:
2242 1083 104
BanI G'GyrC_C
Cuts at: 0 1547 3429
Size: 1547 1882
BanII G_rGCy'C
Cuts at: 0 1142 1297 3429
Size: 1142 155 2132
Fragments arranged by size:
2132 1142 155
BbeI G_GCGC'C
Cuts at: 0 1551 3429
Size: 1551 1878
BbsI GAAGACnn'nnnn_
Cuts at: 0 239 485 2549 2606 3155 3429
Size: 239 246 2064 57 549 274
Fragments arranged by size:
2064 549 274 246 239 57
BciVI GTATCCnnnnn_n'
Cuts at: 0 574 872 1753 3429
Size: 574 298 881 1676
Fragments arranged by size:
1676 881 574 298
BfrBI ATG'CAT
Cuts at: 0 394 1853 3429
Size: 394 1459 1576
Fragments arranged by size:
1576 1459 394
BglII A'GATC_T
Cuts at: 0 2391 3429
Size: 2391 1038
BlpI GC'TnA_GC
Cuts at: 0 1296 3429
Size: 1296 2133
Bme1580I G_kGCm'C
Cuts at: 0 2162 3011 3429
Size: 2162 849 418
Fragments arranged by size:
2162 849 418
BmrI ACTGGGnnnn_n'
Cuts at: 0 190 1230 1280 2254 3429
Size: 190 1040 50 974 1175
Fragments arranged by size:
1175 1040 974 190 50
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 914 1781 3429
Size: 914 867 1648
Fragments arranged by size:
1648 914 867
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 544 853 884 1751 2188 2416 3086 3429
Size: 544 309 31 867 437 228 670 343
Fragments arranged by size:
867 670 544 437 343 309 228 31
Bpu10I CC'TnA_GC
Cuts at: 0 2482 3429
Size: 2482 947
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 652 3429
Size: 652 2777
BsaI GGTCTCn'nnnn_
Cuts at: 0 902 2473 3429
Size: 902 1571 956
Fragments arranged by size:
1571 956 902
BsaHI Gr'CG_yC
Cuts at: 0 1548 3429
Size: 1548 1881
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1591 1621 3429
Size: 1591 30 1808
Fragments arranged by size:
1808 1591 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 531 906 1612 1792 2107 2330 3429
Size: 531 375 706 180 315 223 1099
Fragments arranged by size:
1099 706 531 375 315 223 180
BseYI C'CCAG_C
Cuts at: 0 3037 3429
Size: 3037 392
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 560 1651 1674 2103 2260 3429
Size: 560 1091 23 429 157 1169
Fragments arranged by size:
1169 1091 560 429 157 23
BsiHKAI G_wGCw'C
Cuts at: 0 1142 1297 1505 3011 3429
Size: 1142 155 208 1506 418
Fragments arranged by size:
1506 1142 418 208 155
BsmI GAATG_Cn'
Cuts at: 0 906 3429
Size: 906 2523
BsmBI CGTCTCn'nnnn_
Cuts at: 0 7 3429
Size: 7 3422
Bsp1286I G_dGCh'C
Cuts at: 0 1142 1297 1505 2162 3011 3429
Size: 1142 155 208 657 849 418
Fragments arranged by size:
1142 849 657 418 208 155
BspHI T'CATG_A
Cuts at: 0 2961 3429
Size: 2961 468
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 201 1849 2142 3268 3429
Size: 201 1648 293 1126 161
Fragments arranged by size:
1648 1126 293 201 161
BsrDI GCAATG_nn'
Cuts at: 0 782 1725 2849 3429
Size: 782 943 1124 580
Fragments arranged by size:
1124 943 782 580
BsrFI r'CCGG_y
Cuts at: 0 2034 3429
Size: 2034 1395
BsrGI T'GTAC_A
Cuts at: 0 21 3429
Size: 21 3408
BssSI C'ACGA_G
Cuts at: 0 1290 2429 3429
Size: 1290 1139 1000
Fragments arranged by size:
1290 1139 1000
BstAPI GCAn_nnn'nTGC
Cuts at: 0 3236 3429
Size: 3236 193
BstBI TT'CG_AA
Cuts at: 0 3166 3429
Size: 3166 263
BstEII G'GTnAC_C
Cuts at: 0 2689 3429
Size: 2689 740
BstYI r'GATC_y
Cuts at: 0 1083 1158 1187 2003 2243 2391 3429
Size: 1083 75 29 816 240 148 1038
Fragments arranged by size:
1083 1038 816 240 148 75 29
BstZ17I GTA'TAC
Cuts at: 0 780 3429
Size: 780 2649
BtgI C'CryG_G
Cuts at: 0 729 873 1496 3429
Size: 729 144 623 1933
Fragments arranged by size:
1933 729 623 144
BtsI GCAGTG_nn'
Cuts at: 0 1760 3429
Size: 1760 1669
DraIII CAC_nnn'GTG
Cuts at: 0 2419 3429
Size: 2419 1010
EarI CTCTTCn'nnn_
Cuts at: 0 662 700 965 1130 1778 2963 3083 3356
Size: 662 38 265 165 648 1185 120 273
Cuts at: 3356 3429
Size: 73
Fragments arranged by size:
1185 662 648 273 265 165 120 73
38
EcoICRI GAG'CTC
Cuts at: 0 1140 1295 3429
Size: 1140 155 2134
Fragments arranged by size:
2134 1140 155
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 181 544 803 853 884 1052 1279 1751
Size: 181 363 259 50 31 168 227 472
Cuts at: 1751 2188 2416 2421 2479 2629 2889 3086 3429
Size: 437 228 5 58 150 260 197 343
Fragments arranged by size:
472 437 363 343 260 259 228 227
197 181 168 150 58 50 31 5
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1261 3429
Size: 1261 2168
EcoRI G'AATT_C
Cuts at: 0 3423 3429
Size: 3423 6
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 513 994 3429
Size: 513 481 2435
Fragments arranged by size:
2435 513 481
HaeII r_GCGC'y
Cuts at: 0 1551 1617 2102 3429
Size: 1551 66 485 1327
Fragments arranged by size:
1551 1327 485 66
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 109 141 1279 1311 1385 1417 1647 1679
Size: 109 32 1138 32 74 32 230 32
Cuts at: 1679 2383 2415 2475 2507 3429
Size: 704 32 60 32 922
Fragments arranged by size:
1138 922 704 230 109 74 60 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 2178 3429
Size: 2178 1251
HindIII A'AGCT_T
Cuts at: 0 572 2611 3429
Size: 572 2039 818
Fragments arranged by size:
2039 818 572
KasI G'GCGC_C
Cuts at: 0 1547 3429
Size: 1547 1882
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 27 2108 2519 2831 2864 3429
Size: 27 2081 411 312 33 565
Fragments arranged by size:
2081 565 411 312 33 27
MslI CAynn'nnrTG
Cuts at: 0 721 1627 2020 3004 3014 3429
Size: 721 906 393 984 10 415
Fragments arranged by size:
984 906 721 415 393 10
MspA1I CmG'CkG
Cuts at: 0 1300 1648 2060 2449 2721 3429
Size: 1300 348 412 389 272 708
Fragments arranged by size:
1300 708 412 389 348 272
NaeI GCC'GGC
Cuts at: 0 2036 3429
Size: 2036 1393
NarI GG'CG_CC
Cuts at: 0 1548 3429
Size: 1548 1881
NcoI C'CATG_G
Cuts at: 0 1496 3429
Size: 1496 1933
NgoMIV G'CCGG_C
Cuts at: 0 2034 3429
Size: 2034 1395
NsiI A_TGCA'T
Cuts at: 0 396 1855 3429
Size: 396 1459 1574
Fragments arranged by size:
1574 1459 396
NspI r_CATG'y
Cuts at: 0 1446 1853 2203 3206 3429
Size: 1446 407 350 1003 223
Fragments arranged by size:
1446 1003 407 350 223
PasI CC'CwG_GG
Cuts at: 0 225 3429
Size: 225 3204
PflMI CCAn_nnn'nTGG
Cuts at: 0 202 1004 3429
Size: 202 802 2425
Fragments arranged by size:
2425 802 202
PfoI T'CCnGG_A
Cuts at: 0 857 3429
Size: 857 2572
PshAI GACnn'nnGTC
Cuts at: 0 548 1539 3429
Size: 548 991 1890
Fragments arranged by size:
1890 991 548
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 401 433 3429
Size: 401 32 2996
Fragments arranged by size:
2996 401 32
PstI C_TGCA'G
Cuts at: 0 2411 3429
Size: 2411 1018
PvuII CAG'CTG
Cuts at: 0 1300 2060 2449 3429
Size: 1300 760 389 980
Fragments arranged by size:
1300 980 760 389
SacI G_AGCT'C
Cuts at: 0 1142 1297 3429
Size: 1142 155 2132
Fragments arranged by size:
2132 1142 155
SapI GCTCTTCn'nnn_
Cuts at: 0 1130 3083 3429
Size: 1130 1953 346
Fragments arranged by size:
1953 1130 346
ScaI AGT'ACT
Cuts at: 0 1219 3429
Size: 1219 2210
SexAI A'CCwGG_T
Cuts at: 0 448 3429
Size: 448 2981
SfcI C'TryA_G
Cuts at: 0 418 1143 2407 3429
Size: 418 725 1264 1022
Fragments arranged by size:
1264 1022 725 418
SfoI GGC'GCC
Cuts at: 0 1549 3429
Size: 1549 1880
SmlI C'TyrA_G
Cuts at: 0 631 3429
Size: 631 2798
SphI G_CATG'C
Cuts at: 0 1853 2203 3429
Size: 1853 350 1226
Fragments arranged by size:
1853 1226 350
SspI AAT'ATT
Cuts at: 0 648 3429
Size: 648 2781
StuI AGG'CCT
Cuts at: 0 85 464 2684 2927 2955 3429
Size: 85 379 2220 243 28 474
Fragments arranged by size:
2220 474 379 243 85 28
StyI C'CwwG_G
Cuts at: 0 473 1191 1496 2685 3429
Size: 473 718 305 1189 744
Fragments arranged by size:
1189 744 718 473 305
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 194 1376 3429
Size: 194 1182 2053
Fragments arranged by size:
2053 1182 194
TatI w'GTAC_w
Cuts at: 0 21 1217 3018 3429
Size: 21 1196 1801 411
Fragments arranged by size:
1801 1196 411 21
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1179 1211 1591 1623 2415 2447 3429
Size: 1179 32 380 32 792 32 982
Fragments arranged by size:
1179 982 792 380 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 2857 3429
Size: 2857 572
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 203 3429
Size: 203 3226
XmnI GAAnn'nnTTC
Cuts at: 0 1070 3380 3429
Size: 1070 2310 49
Fragments arranged by size:
2310 1070 49
Enzymes that do cut:
AarI AccI AcuI AfeI AleI AloI AlwNI ApaLI
ApoI AseI BamHI BanI BanII BbeI BbsI BciVI
BfrBI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI
BsmI BsmBI Bsp1286I BspHI BspMI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtsI
DraIII EarI EcoICRI Eco57MI EcoNI EcoRI FalI HaeII
Hin4I HincII HindIII KasI MmeI MslI MspA1I NaeI
NarI NcoI NgoMIV NsiI NspI PasI PflMI PfoI
PshAI PsrI PstI PvuII SacI SapI ScaI SexAI
SfcI SfoI SmlI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflII AflIII AgeI AhdI AlfI
ApaI AscI AsiSI AvaI AvrII BaeI BbvCI BcgI
BclI BglI BmgBI BmtI BsaAI BsaBI BsaWI BsiEI
BsiWI BspEI BsrBI BssHII BstXI Bsu36I BtgZI ClaI
CspCI DraI DrdI EaeI EagI EciI EcoO109I EcoRV
FseI FspI FspAI HpaI KpnI MfeI MluI MscI
NdeI NheI NotI NruI PacI PciI PmeI PmlI
PpiI PpuMI PsiI PspOMI PspXI PvuI RsrII SacII
SalI SanDI SbfI SfiI SgfI SgrAI SmaI SnaBI
SpeI SrfI SwaI XbaI XhoI XmaI ZraI