Restriction map for
pFN21AE3693
(Linear) (Six-base) MAPSORT of: KIEE3693.seq Check: 5701 from: 1 to: 2865
KIEE3693.orf
With 182 enzymes: SgfI *
July 3, 2012 18:14 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 998 2408 2865
Size: 998 1410 457
Fragments arranged by size:
1410 998 457
AgeI A'CCGG_T
Cuts at: 0 455 2865
Size: 455 2410
AlwNI CAG_nnn'CTG
Cuts at: 0 718 2865
Size: 718 2147
ApaI G_GGCC'C
Cuts at: 0 2769 2865
Size: 2769 96
ApoI r'AATT_y
Cuts at: 0 1351 1557 2865
Size: 1351 206 1308
Fragments arranged by size:
1351 1308 206
AvaI C'yCGr_G
Cuts at: 0 731 1691 2096 2228 2489 2714 2865
Size: 731 960 405 132 261 225 151
Fragments arranged by size:
960 731 405 261 225 151 132
BanI G'GyrC_C
Cuts at: 0 625 868 2865
Size: 625 243 1997
Fragments arranged by size:
1997 625 243
BanII G_rGCy'C
Cuts at: 0 658 853 946 2490 2769 2865
Size: 658 195 93 1544 279 96
Fragments arranged by size:
1544 658 279 195 96 93
BbsI GAAGACnn'nnnn_
Cuts at: 0 440 1063 1667 2810 2865
Size: 440 623 604 1143 55
Fragments arranged by size:
1143 623 604 440 55
BbvCI CC'TCA_GC
Cuts at: 0 708 2117 2865
Size: 708 1409 748
Fragments arranged by size:
1409 748 708
BclI T'GATC_A
Cuts at: 0 1293 2865
Size: 1293 1572
BglII A'GATC_T
Cuts at: 0 49 61 1785 2190 2865
Size: 49 12 1724 405 675
Fragments arranged by size:
1724 675 405 49 12
Bme1580I G_kGCm'C
Cuts at: 0 1535 2769 2865
Size: 1535 1234 96
Fragments arranged by size:
1535 1234 96
BmrI ACTGGGnnnn_n'
Cuts at: 0 1742 2042 2346 2449 2865
Size: 1742 300 304 103 416
Fragments arranged by size:
1742 416 304 300 103
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1614 2865
Size: 1614 1251
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 149 641 748 1418 1489 2220 2865
Size: 149 492 107 670 71 731 645
Fragments arranged by size:
731 670 645 492 149 107 71
Bpu10I CC'TnA_GC
Cuts at: 0 708 1313 1763 2117 2865
Size: 708 605 450 354 748
Fragments arranged by size:
748 708 605 450 354
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 30 617 2229 2865
Size: 30 587 1612 636
Fragments arranged by size:
1612 636 587 30
BsaI GGTCTCn'nnnn_
Cuts at: 0 149 2865
Size: 149 2716
BsaBI GATnn'nnATC
Cuts at: 0 54 2865
Size: 54 2811
BsaWI w'CCGG_w
Cuts at: 0 455 1712 2738 2865
Size: 455 1257 1026 127
Fragments arranged by size:
1257 1026 455 127
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 526 556 1384 1414 1462 1492 2865
Size: 526 30 828 30 48 30 1373
Fragments arranged by size:
1373 828 526 48 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 317 459 1015 1034 1297 1405 1408 1477
Size: 317 142 556 19 263 108 3 69
Cuts at: 1477 1486 1489 2328 2331 2422 2489 2614 2717
Size: 9 3 839 3 91 67 125 103
Cuts at: 2717 2865
Size: 148
Fragments arranged by size:
839 556 317 263 148 142 125 108
103 91 69 67 19 9 3 3
3
BseYI C'CCAG_C
Cuts at: 0 744 1641 2525 2865
Size: 744 897 884 340
Fragments arranged by size:
897 884 744 340
BsiHKAI G_wGCw'C
Cuts at: 0 658 2416 2490 2865
Size: 658 1758 74 375
Fragments arranged by size:
1758 658 375 74
Bsp1286I G_dGCh'C
Cuts at: 0 658 853 946 1535 2416 2490 2769 2865
Size: 658 195 93 589 881 74 279 96
Fragments arranged by size:
881 658 589 279 195 96 93 74
BspEI T'CCGG_A
Cuts at: 0 1712 2738 2865
Size: 1712 1026 127
Fragments arranged by size:
1712 1026 127
BspHI T'CATG_A
Cuts at: 0 2076 2865
Size: 2076 789
BsrBI CCG'CTC
Cuts at: 0 323 2865
Size: 323 2542
BsrFI r'CCGG_y
Cuts at: 0 341 455 560 2726 2865
Size: 341 114 105 2166 139
Fragments arranged by size:
2166 341 139 114 105
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1778 2865
Size: 1778 1087
BstBI TT'CG_AA
Cuts at: 0 82 2865
Size: 82 2783
BstEII G'GTnAC_C
Cuts at: 0 788 2865
Size: 788 2077
BstXI CCAn_nnnn'nTGG
Cuts at: 0 916 2865
Size: 916 1949
BstYI r'GATC_y
Cuts at: 0 28 49 61 376 1492 1785 2190 2332
Size: 28 21 12 315 1116 293 405 142
Cuts at: 2332 2865
Size: 533
Fragments arranged by size:
1116 533 405 315 293 142 28 21
12
Bsu36I CC'TnA_GG
Cuts at: 0 1935 2865
Size: 1935 930
BtgI C'CryG_G
Cuts at: 0 258 283 664 909 2417 2865
Size: 258 25 381 245 1508 448
Fragments arranged by size:
1508 448 381 258 245 25
BtsI GCAGTG_nn'
Cuts at: 0 2676 2865
Size: 2676 189
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2406 2441 2763 2798 2865
Size: 2406 35 322 35 67
Fragments arranged by size:
2406 322 67 35 35
EaeI y'GGCC_r
Cuts at: 0 562 667 2865
Size: 562 105 2198
Fragments arranged by size:
2198 562 105
EarI CTCTTCn'nnn_
Cuts at: 0 42 552 1043 2349 2372 2384 2514 2591
Size: 42 510 491 1306 23 12 130 77
Cuts at: 2591 2828 2865
Size: 237 37
Fragments arranged by size:
1306 510 491 237 130 77 42 37
23 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2140 2865
Size: 2140 725
EcoICRI GAG'CTC
Cuts at: 0 656 2488 2865
Size: 656 1832 377
Fragments arranged by size:
1832 656 377
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 149 641 748 998 1418 1489 2220 2408
Size: 149 492 107 250 420 71 731 188
Cuts at: 2408 2865
Size: 457
Fragments arranged by size:
731 492 457 420 250 188 149 107
71
EcoNI CCTnn'n_nnAGG
Cuts at: 0 362 2865
Size: 362 2503
EcoO109I rG'GnC_Cy
Cuts at: 0 245 398 920 1416 1734 1931 2607 2865
Size: 245 153 522 496 318 197 676 258
Fragments arranged by size:
676 522 496 318 258 245 197 153
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 419 1695 2658 2865
Size: 419 1276 963 207
Fragments arranged by size:
1276 963 419 207
HaeII r_GCGC'y
Cuts at: 0 468 2865
Size: 468 2397
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 430 462 526 558 1278 1310 1384 1416
Size: 430 32 64 32 720 32 74 32
Cuts at: 1416 1462 1494 1775 1807 2865
Size: 46 32 281 32 1058
Fragments arranged by size:
1058 720 430 281 74 64 46 32
32 32 32 32 32
HindIII A'AGCT_T
Cuts at: 0 1774 2865
Size: 1774 1091
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 515 2865
Size: 515 2350
MscI TGG'CCA
Cuts at: 0 669 2865
Size: 669 2196
MspA1I CmG'CkG
Cuts at: 0 2260 2865
Size: 2260 605
NaeI GCC'GGC
Cuts at: 0 562 2865
Size: 562 2303
NcoI C'CATG_G
Cuts at: 0 283 664 909 2865
Size: 283 381 245 1956
Fragments arranged by size:
1956 381 283 245
NdeI CA'TA_TG
Cuts at: 0 981 2865
Size: 981 1884
NgoMIV G'CCGG_C
Cuts at: 0 560 2865
Size: 560 2305
PflMI CCAn_nnn'nTGG
Cuts at: 0 771 970 2865
Size: 771 199 1895
Fragments arranged by size:
1895 771 199
PfoI T'CCnGG_A
Cuts at: 0 415 1586 2865
Size: 415 1171 1279
Fragments arranged by size:
1279 1171 415
PpuMI rG'GwC_Cy
Cuts at: 0 398 920 1734 1931 2607 2865
Size: 398 522 814 197 676 258
Fragments arranged by size:
814 676 522 398 258 197
PshAI GACnn'nnGTC
Cuts at: 0 2237 2865
Size: 2237 628
PsiI TTA'TAA
Cuts at: 0 1577 2865
Size: 1577 1288
PspOMI G'GGCC_C
Cuts at: 0 2765 2865
Size: 2765 100
PspXI vC'TCGA_Gb
Cuts at: 0 2489 2865
Size: 2489 376
PvuII CAG'CTG
Cuts at: 0 2260 2865
Size: 2260 605
SacI G_AGCT'C
Cuts at: 0 658 2490 2865
Size: 658 1832 375
Fragments arranged by size:
1832 658 375
SanDI GG'GwC_CC
Cuts at: 0 920 2607 2865
Size: 920 1687 258
Fragments arranged by size:
1687 920 258
SapI GCTCTTCn'nnn_
Cuts at: 0 42 1043 2865
Size: 42 1001 1822
Fragments arranged by size:
1822 1001 42
SfcI C'TryA_G
Cuts at: 0 237 2865
Size: 237 2628
SmaI CCC'GGG
Cuts at: 0 2098 2865
Size: 2098 767
SmlI C'TyrA_G
Cuts at: 0 45 632 1691 2244 2489 2865
Size: 45 587 1059 553 245 376
Fragments arranged by size:
1059 587 553 376 245 45
StyI C'CwwG_G
Cuts at: 0 283 409 602 664 894 909 2865
Size: 283 126 193 62 230 15 1956
Fragments arranged by size:
1956 283 230 193 126 62 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 80 206 1138 2637 2670 2865
Size: 80 126 932 1499 33 195
Fragments arranged by size:
1499 932 195 126 80 33
TatI w'GTAC_w
Cuts at: 0 2050 2381 2615 2865
Size: 2050 331 234 250
Fragments arranged by size:
2050 331 250 234
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1915 1947 2587 2619 2865
Size: 1915 32 640 32 246
Fragments arranged by size:
1915 640 246 32 32
XbaI T'CTAG_A
Cuts at: 0 427 1044 2865
Size: 427 617 1821
Fragments arranged by size:
1821 617 427
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1755 2454 2865
Size: 1755 699 411
Fragments arranged by size:
1755 699 411
XhoI C'TCGA_G
Cuts at: 0 1691 2489 2865
Size: 1691 798 376
Fragments arranged by size:
1691 798 376
XmaI C'CCGG_G
Cuts at: 0 2096 2865
Size: 2096 769
XmnI GAAnn'nnTTC
Cuts at: 0 1558 1674 2865
Size: 1558 116 1191
Fragments arranged by size:
1558 1191 116
Enzymes that do cut:
AcuI AgeI AlwNI ApaI ApoI AvaI BanI BanII
BbsI BbvCI BclI BglII Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaBI BsaWI BsaXI BseRI BseYI
BsiHKAI Bsp1286I BspEI BspHI BsrBI BsrFI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtsI CspCI EaeI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII
Hin4I HindIII MmeI MscI MspA1I NaeI NcoI NdeI
NgoMIV PflMI PfoI PpuMI PshAI PsiI PspOMI PspXI
PvuII SacI SanDI SapI SfcI SmaI SmlI StyI
TaqII TatI TstI XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BcgI BciVI BfrBI BglI
BlpI BmgBI BmtI BsaAI BsaHI BsgI BsiEI BsiWI
BsmI BsmBI BspMI BsrDI BsrGI BssHII BssSI BstZ17I
BtgZI ClaI DraI DraIII DrdI EagI EcoRI EcoRV
FseI FspI FspAI HincII HpaI KasI KpnI MfeI
MluI MslI NarI NheI NotI NruI NsiI NspI
PacI PasI PciI PmeI PmlI PpiI PsrI PstI
PvuI RsrII SacII SalI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI
StuI SwaI Tth111I ZraI