(Linear) (Six-base) MAPSORT of: KIEE3692.seq  Check: 796  from: 1  to: 546



KIEE3692.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 16:40  ..

AatII G_ACGT'C

Cuts at:      0     396     546 
   Size:        396     150
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     276     546 
   Size:        276     270
 
AflIII A'CryG_T

Cuts at:      0     130     464     546 
   Size:        130     334      82

  Fragments arranged by size: 

                 334     130      82
 
ApaI G_GGCC'C

Cuts at:      0      89     488     546 
   Size:         89     399      58

  Fragments arranged by size: 

                 399      89      58
 
BamHI G'GATC_C

Cuts at:      0     383     546 
   Size:        383     163
 
BanI G'GyrC_C

Cuts at:      0      91     546 
   Size:         91     455
 
BanII G_rGCy'C

Cuts at:      0      89     488     546 
   Size:         89     399      58

  Fragments arranged by size: 

                 399      89      58
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     393     546 
   Size:        393     153
 
BglI GCCn_nnn'nGGC

Cuts at:      0      74     546 
   Size:         74     472
 
Bme1580I G_kGCm'C

Cuts at:      0      89     488     546 
   Size:         89     399      58

  Fragments arranged by size: 

                 399      89      58
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     115     332     546 
   Size:        115     217     214

  Fragments arranged by size: 

                 217     214     115
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     125     546 
   Size:        125     421
 
BsaAI yAC'GTr

Cuts at:      0     465     546 
   Size:        465      81
 
BsaHI Gr'CG_yC

Cuts at:      0     393     546 
   Size:        393     153
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     103     133     546 
   Size:        103      30     413

  Fragments arranged by size: 

                 413     103      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0       1     546 
   Size:          1     545
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     384     546 
   Size:        384     162
 
Bsp1286I G_dGCh'C

Cuts at:      0      89     488     546 
   Size:         89     399      58

  Fragments arranged by size: 

                 399      89      58
 
BsrDI GCAATG_nn'

Cuts at:      0     264     546 
   Size:        264     282
 
BstYI r'GATC_y

Cuts at:      0     230     383     546 
   Size:        230     153     163

  Fragments arranged by size: 

                 230     163     153
 
BtsI GCAGTG_nn'

Cuts at:      0     448     546 
   Size:        448      98
 
DraIII CAC_nnn'GTG

Cuts at:      0     326     546 
   Size:        326     220
 
EarI CTCTTCn'nnn_

Cuts at:      0     295     546 
   Size:        295     251
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     276     546 
   Size:        276     270
 
EcoO109I rG'GnC_Cy

Cuts at:      0      27     546 
   Size:         27     519
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     103     135     546 
   Size:        103      32     411

  Fragments arranged by size: 

                 411     103      32
 
PmlI CAC'GTG

Cuts at:      0     465     546 
   Size:        465      81
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      33      65     546 
   Size:         33      32     481

  Fragments arranged by size: 

                 481      33      32
 
PspOMI G'GGCC_C

Cuts at:      0      85     484     546 
   Size:         85     399      62

  Fragments arranged by size: 

                 399      85      62
 
ScaI AGT'ACT

Cuts at:      0     310     546 
   Size:        310     236
 
StyI C'CwwG_G

Cuts at:      0     535     546 
   Size:        535      11
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     314     546 
   Size:        314     232
 
TatI w'GTAC_w

Cuts at:      0     308     546 
   Size:        308     238
 
ZraI GAC'GTC

Cuts at:      0     394     546 
   Size:        394     152
 

 Enzymes that do cut:

    AatII     AcuI   AflIII     ApaI    BamHI     BanI    BanII    BciVI
     BglI Bme1580I     BmrI     BsaI    BsaAI    BsaHI    BsaXI    BseRI
    BsmBI Bsp1286I    BsrDI    BstYI     BtsI   DraIII     EarI  Eco57MI
 EcoO109I    Hin4I     PmlI     PpiI   PspOMI     ScaI     StyI    TaqII
     TatI     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI    ApaLI     ApoI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI     BbeI     BbsI    BbvCI     BcgI
     BclI    BfrBI    BglII     BlpI    BmgBI     BmtI     BplI     BpmI
   Bpu10I    BpuEI    BsaBI    BsaWI    BseYI     BsgI    BsiEI  BsiHKAI
    BsiWI     BsmI    BspEI    BspHI    BspMI    BsrBI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
     BtgI    BtgZI     ClaI    CspCI     DraI     DrdI     EaeI     EagI
     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PfoI     PmeI    PpuMI    PshAI     PsiI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI    SexAI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI