(Linear) (Six-base) MAPSORT of: KIEE3685.seq  Check: 3490  from: 1  to: 960



KIEE3685.orf

 With 182 enzymes: SgfI * 

                        December 27, 2011 16:38  ..

AccI GT'mk_AC

Cuts at:      0       4     960 
   Size:          4     956
 
AclI AA'CG_TT

Cuts at:      0     741     960 
   Size:        741     219
 
ApoI r'AATT_y

Cuts at:      0      12     960 
   Size:         12     948
 
BanII G_rGCy'C

Cuts at:      0     796     960 
   Size:        796     164
 
BglII A'GATC_T

Cuts at:      0     486     718     748     814     871     960 
   Size:        486     232      30      66      57      89

  Fragments arranged by size: 

                 486     232      89      66      57      30
 
Bme1580I G_kGCm'C

Cuts at:      0     148     960 
   Size:        148     812
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     762     960 
   Size:        762     198
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     645     960 
   Size:        645     315
 
BsaBI GATnn'nnATC

Cuts at:      0     485     639     960 
   Size:        485     154     321

  Fragments arranged by size: 

                 485     321     154
 
BseYI C'CCAG_C

Cuts at:      0     139     960 
   Size:        139     821
 
Bsp1286I G_dGCh'C

Cuts at:      0     148     796     960 
   Size:        148     648     164

  Fragments arranged by size: 

                 648     164     148
 
BsrDI GCAATG_nn'

Cuts at:      0     214     231     960 
   Size:        214      17     729

  Fragments arranged by size: 

                 729     214      17
 
BssSI C'ACGA_G

Cuts at:      0     752     960 
   Size:        752     208
 
BstEII G'GTnAC_C

Cuts at:      0     917     960 
   Size:        917      43
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     937     960 
   Size:        937      23
 
BstYI r'GATC_y

Cuts at:      0     486     619     718     748     769     814     871     960 
   Size:        486     133      99      30      21      45      57      89

  Fragments arranged by size: 

                 486     133      99      89      57      45      30      21
            
 
ClaI AT'CG_AT

Cuts at:      0      96     960 
   Size:         96     864
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0      39      74     960 
   Size:         39      35     886

  Fragments arranged by size: 

                 886      39      35
 
EarI CTCTTCn'nnn_

Cuts at:      0     464     801     960 
   Size:        464     337     159

  Fragments arranged by size: 

                 464     337     159
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     762     960 
   Size:        762     198
 
EcoO109I rG'GnC_Cy

Cuts at:      0     683     782     960 
   Size:        683      99     178

  Fragments arranged by size: 

                 683     178      99
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     716     960 
   Size:        716     244
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     439     471     960 
   Size:        439      32     489

  Fragments arranged by size: 

                 489     439      32
 
HincII GTy'rAC

Cuts at:      0       5     960 
   Size:          5     955
 
MspA1I CmG'CkG

Cuts at:      0      89     960 
   Size:         89     871
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     849     881     960 
   Size:        849      32      79

  Fragments arranged by size: 

                 849      79      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     683     782     960 
   Size:        683      99     178

  Fragments arranged by size: 

                 683     178      99
 
PvuII CAG'CTG

Cuts at:      0      89     960 
   Size:         89     871
 
SalI G'TCGA_C

Cuts at:      0       3     960 
   Size:          3     957
 
SapI GCTCTTCn'nnn_

Cuts at:      0     801     960 
   Size:        801     159
 
SmlI C'TyrA_G

Cuts at:      0     660     960 
   Size:        660     300
 
StuI AGG'CCT

Cuts at:      0     185     960 
   Size:        185     775
 
Tth111I GACn'n_nGTC

Cuts at:      0     602     960 
   Size:        602     358
 
XbaI T'CTAG_A

Cuts at:      0     868     960 
   Size:        868      92
 
XmnI GAAnn'nnTTC

Cuts at:      0     699     864     960 
   Size:        699     165      96

  Fragments arranged by size: 

                 699     165      96
 

 Enzymes that do cut:

     AccI     AclI     ApoI    BanII    BglII Bme1580I     BpmI    BpuEI
    BsaBI    BseYI Bsp1286I    BsrDI    BssSI   BstEII    BstXI    BstYI
     ClaI    CspCI     EarI  Eco57MI EcoO109I     FalI    Hin4I   HincII
   MspA1I     PpiI    PpuMI    PvuII     SalI     SapI     SmlI     StuI
  Tth111I     XbaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AcuI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI     BlpI
    BmgBI     BmrI     BmtI     BplI   Bpu10I     BsaI    BsaAI    BsaHI
    BsaWI    BsaXI    BseRI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrFI    BsrGI   BssHII
   BstAPI    BstBI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     DraI
   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI    EcoRI
    EcoRV     FseI     FspI    FspAI    HaeII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MscI     MslI     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII     SacI
    SacII    SanDI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     StyI     SwaI    TaqII     TatI     TstI     XcmI     XhoI     XmaI
     ZraI