Restriction map for
pFN21AE3684
(Linear) (Six-base) MAPSORT of: KIEE3684.seq Check: 7725 from: 1 to: 1467
KIEE3684.orf
With 182 enzymes: SgfI *
March 22, 2012 16:39 ..
Acc65I G'GTAC_C
Cuts at: 0 1397 1467
Size: 1397 70
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 325 568 1467
Size: 325 243 899
Fragments arranged by size:
899 325 243
AgeI A'CCGG_T
Cuts at: 0 1394 1467
Size: 1394 73
AleI CACnn'nnGTG
Cuts at: 0 1024 1467
Size: 1024 443
AlwNI CAG_nnn'CTG
Cuts at: 0 1047 1467
Size: 1047 420
ApaI G_GGCC'C
Cuts at: 0 334 684 1467
Size: 334 350 783
Fragments arranged by size:
783 350 334
ApoI r'AATT_y
Cuts at: 0 933 1467
Size: 933 534
AvaI C'yCGr_G
Cuts at: 0 713 770 920 1467
Size: 713 57 150 547
Fragments arranged by size:
713 547 150 57
BamHI G'GATC_C
Cuts at: 0 166 1467
Size: 166 1301
BanI G'GyrC_C
Cuts at: 0 86 1397 1467
Size: 86 1311 70
Fragments arranged by size:
1311 86 70
BanII G_rGCy'C
Cuts at: 0 73 295 334 478 499 684 1467
Size: 73 222 39 144 21 185 783
Fragments arranged by size:
783 222 185 144 73 39 21
BbeI G_GCGC'C
Cuts at: 0 90 1467
Size: 90 1377
BbvCI CC'TCA_GC
Cuts at: 0 949 1467
Size: 949 518
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 357 391 1467
Size: 357 34 1076
Fragments arranged by size:
1076 357 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 812 1467
Size: 812 655
BglI GCCn_nnn'nGGC
Cuts at: 0 1417 1467
Size: 1417 50
Bme1580I G_kGCm'C
Cuts at: 0 334 684 1467
Size: 334 350 783
Fragments arranged by size:
783 350 334
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 309 1467
Size: 309 1158
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 383 1225 1422 1467
Size: 383 842 197 45
Fragments arranged by size:
842 383 197 45
Bpu10I CC'TnA_GC
Cuts at: 0 67 121 949 1467
Size: 67 54 828 518
Fragments arranged by size:
828 518 67 54
BsaI GGTCTCn'nnnn_
Cuts at: 0 335 1467
Size: 335 1132
BsaHI Gr'CG_yC
Cuts at: 0 87 1376 1467
Size: 87 1289 91
Fragments arranged by size:
1289 91 87
BsaWI w'CCGG_w
Cuts at: 0 1394 1467
Size: 1394 73
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 880 910 1243 1273 1467
Size: 880 30 333 30 194
Fragments arranged by size:
880 333 194 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 205 208 211 220 229 244 327 607
Size: 205 3 3 9 9 15 83 280
Cuts at: 607 610 613 688 938 1467
Size: 3 3 75 250 529
Fragments arranged by size:
529 280 250 205 83 75 15 9
9 3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 477 498 1077 1467
Size: 477 21 579 390
Fragments arranged by size:
579 477 390 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 802 1207 1467
Size: 802 405 260
Fragments arranged by size:
802 405 260
BsiEI CG_ry'CG
Cuts at: 0 1362 1467
Size: 1362 105
BsiHKAI G_wGCw'C
Cuts at: 0 295 728 1467
Size: 295 433 739
Fragments arranged by size:
739 433 295
BsmBI CGTCTCn'nnnn_
Cuts at: 0 692 1467
Size: 692 775
Bsp1286I G_dGCh'C
Cuts at: 0 73 295 334 478 499 684 728 1467
Size: 73 222 39 144 21 185 44 739
Fragments arranged by size:
739 222 185 144 73 44 39 21
BsrFI r'CCGG_y
Cuts at: 0 761 1394 1467
Size: 761 633 73
Fragments arranged by size:
761 633 73
BstEII G'GTnAC_C
Cuts at: 0 1287 1467
Size: 1287 180
BstXI CCAn_nnnn'nTGG
Cuts at: 0 513 963 1077 1241 1467
Size: 513 450 114 164 226
Fragments arranged by size:
513 450 226 164 114
BstYI r'GATC_y
Cuts at: 0 166 1467
Size: 166 1301
Bsu36I CC'TnA_GG
Cuts at: 0 468 1467
Size: 468 999
BtgI C'CryG_G
Cuts at: 0 82 161 695 1086 1467
Size: 82 79 534 391 381
Fragments arranged by size:
534 391 381 82 79
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1413 1467
Size: 1413 54
DraIII CAC_nnn'GTG
Cuts at: 0 1009 1467
Size: 1009 458
EaeI y'GGCC_r
Cuts at: 0 1359 1467
Size: 1359 108
EagI C'GGCC_G
Cuts at: 0 1359 1467
Size: 1359 108
EarI CTCTTCn'nnn_
Cuts at: 0 124 197 221 283 487 584 1467
Size: 124 73 24 62 204 97 883
Fragments arranged by size:
883 204 124 97 73 62 24
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1384 1467
Size: 1384 83
EcoICRI GAG'CTC
Cuts at: 0 293 1467
Size: 293 1174
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 325 383 568 1225 1422 1467
Size: 325 58 185 657 197 45
Fragments arranged by size:
657 325 197 185 58 45
EcoNI CCTnn'n_nnAGG
Cuts at: 0 25 1039 1467
Size: 25 1014 428
Fragments arranged by size:
1014 428 25
EcoO109I rG'GnC_Cy
Cuts at: 0 78 141 330 462 680 681 1143 1467
Size: 78 63 189 132 218 1 462 324
Fragments arranged by size:
462 324 218 189 132 78 63 1
HaeII r_GCGC'y
Cuts at: 0 90 1467
Size: 90 1377
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1243 1275 1467
Size: 1243 32 192
Fragments arranged by size:
1243 192 32
KasI G'GCGC_C
Cuts at: 0 86 1467
Size: 86 1381
KpnI G_GTAC'C
Cuts at: 0 1401 1467
Size: 1401 66
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 980 1467
Size: 980 487
MslI CAynn'nnrTG
Cuts at: 0 1024 1328 1467
Size: 1024 304 139
Fragments arranged by size:
1024 304 139
MspA1I CmG'CkG
Cuts at: 0 84 1054 1404 1467
Size: 84 970 350 63
Fragments arranged by size:
970 350 84 63
NaeI GCC'GGC
Cuts at: 0 763 1467
Size: 763 704
NarI GG'CG_CC
Cuts at: 0 87 1467
Size: 87 1380
NgoMIV G'CCGG_C
Cuts at: 0 761 1467
Size: 761 706
NspI r_CATG'y
Cuts at: 0 801 1467
Size: 801 666
PasI CC'CwG_GG
Cuts at: 0 327 741 1467
Size: 327 414 726
Fragments arranged by size:
726 414 327
PflMI CCAn_nnn'nTGG
Cuts at: 0 1009 1467
Size: 1009 458
PpuMI rG'GwC_Cy
Cuts at: 0 462 1143 1467
Size: 462 681 324
Fragments arranged by size:
681 462 324
PspOMI G'GGCC_C
Cuts at: 0 330 680 1467
Size: 330 350 787
Fragments arranged by size:
787 350 330
PstI C_TGCA'G
Cuts at: 0 1355 1418 1467
Size: 1355 63 49
Fragments arranged by size:
1355 63 49
PvuII CAG'CTG
Cuts at: 0 1054 1467
Size: 1054 413
SacI G_AGCT'C
Cuts at: 0 295 1467
Size: 295 1172
SacII CC_GC'GG
Cuts at: 0 85 1467
Size: 85 1382
SanDI GG'GwC_CC
Cuts at: 0 1143 1467
Size: 1143 324
SapI GCTCTTCn'nnn_
Cuts at: 0 283 487 1467
Size: 283 204 980
Fragments arranged by size:
980 283 204
SbfI CC_TGCA'GG
Cuts at: 0 1418 1467
Size: 1418 49
SfcI C'TryA_G
Cuts at: 0 1351 1414 1467
Size: 1351 63 53
Fragments arranged by size:
1351 63 53
SfoI GGC'GCC
Cuts at: 0 88 1467
Size: 88 1379
SmaI CCC'GGG
Cuts at: 0 715 772 1467
Size: 715 57 695
Fragments arranged by size:
715 695 57
StuI AGG'CCT
Cuts at: 0 1297 1420 1467
Size: 1297 123 47
Fragments arranged by size:
1297 123 47
StyI C'CwwG_G
Cuts at: 0 320 334 1278 1467
Size: 320 14 944 189
Fragments arranged by size:
944 320 189 14
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1306 1467
Size: 1306 161
XmaI C'CCGG_G
Cuts at: 0 713 770 1467
Size: 713 57 697
Fragments arranged by size:
713 697 57
XmnI GAAnn'nnTTC
Cuts at: 0 841 973 1467
Size: 841 132 494
Fragments arranged by size:
841 494 132
Enzymes that do cut:
Acc65I AcuI AgeI AleI AlwNI ApaI ApoI AvaI
BamHI BanI BanII BbeI BbvCI BcgI BciVI BglI
Bme1580I BplI BpmI Bpu10I BsaI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BsrFI
BstEII BstXI BstYI Bsu36I BtgI BtgZI DraIII EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII
Hin4I KasI KpnI MmeI MslI MspA1I NaeI NarI
NgoMIV NspI PasI PflMI PpuMI PspOMI PstI PvuII
SacI SacII SanDI SapI SbfI SfcI SfoI SmaI
StuI StyI TaqII XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AhdI
AlfI AloI ApaLI AscI AseI AsiSI AvrII BaeI
BbsI BclI BfrBI BglII BlpI BmgBI BmrI BmtI
BpuEI BsaAI BsaBI BsiWI BsmI BspEI BspHI BspMI
BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstZ17I
BtsI ClaI CspCI DraI DrdI EcoRI EcoRV FalI
FseI FspI FspAI HincII HindIII HpaI MfeI MluI
MscI NcoI NdeI NheI NotI NruI NsiI PacI
PciI PfoI PmeI PmlI PpiI PshAI PsiI PspXI
PsrI PvuI RsrII SalI ScaI SexAI SfiI SgfI
SgrAI SmlI SnaBI SpeI SphI SrfI SspI SwaI
TatI TstI Tth111I XbaI XcmI XhoI ZraI