Restriction map for
pFN21AE3682
(Linear) (Six-base) MAPSORT of: KIEE3682.seq Check: 5258 from: 1 to: 1251
KIEE3682.orf
With 182 enzymes: SgfI *
December 27, 2011 16:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 168 650 1069 1251
Size: 168 482 419 182
Fragments arranged by size:
482 419 182 168
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 320 1251
Size: 320 931
AflIII A'CryG_T
Cuts at: 0 569 1251
Size: 569 682
AlwNI CAG_nnn'CTG
Cuts at: 0 1020 1251
Size: 1020 231
ApaI G_GGCC'C
Cuts at: 0 717 1251
Size: 717 534
AvaI C'yCGr_G
Cuts at: 0 209 237 630 738 763 1251
Size: 209 28 393 108 25 488
Fragments arranged by size:
488 393 209 108 28 25
BanI G'GyrC_C
Cuts at: 0 259 273 840 879 1225 1251
Size: 259 14 567 39 346 26
Fragments arranged by size:
567 346 259 39 26 14
BanII G_rGCy'C
Cuts at: 0 639 717 1251
Size: 639 78 534
Fragments arranged by size:
639 534 78
BbsI GAAGACnn'nnnn_
Cuts at: 0 74 113 125 1251
Size: 74 39 12 1126
Fragments arranged by size:
1126 74 39 12
BbvCI CC'TCA_GC
Cuts at: 0 913 1251
Size: 913 338
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 784 818 1251
Size: 784 34 433
Fragments arranged by size:
784 433 34
BfrBI ATG'CAT
Cuts at: 0 964 1251
Size: 964 287
BglI GCCn_nnn'nGGC
Cuts at: 0 527 1242 1251
Size: 527 715 9
Fragments arranged by size:
715 527 9
BlpI GC'TnA_GC
Cuts at: 0 17 391 1251
Size: 17 374 860
Fragments arranged by size:
860 374 17
Bme1580I G_kGCm'C
Cuts at: 0 276 717 843 1228 1251
Size: 276 441 126 385 23
Fragments arranged by size:
441 385 276 126 23
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 564 1050 1251
Size: 148 416 486 201
Fragments arranged by size:
486 416 201 148
Bpu10I CC'TnA_GC
Cuts at: 0 913 1251
Size: 913 338
BsaHI Gr'CG_yC
Cuts at: 0 771 942 1251
Size: 771 171 309
Fragments arranged by size:
771 309 171
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 544 574 1251
Size: 544 30 677
Fragments arranged by size:
677 544 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 109 139 142 939 1188 1251
Size: 109 30 3 797 249 63
Fragments arranged by size:
797 249 109 63 30 3
BseYI C'CCAG_C
Cuts at: 0 1217 1251
Size: 1217 34
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 653 667 772 1251
Size: 653 14 105 479
Fragments arranged by size:
653 479 105 14
BsiEI CG_ry'CG
Cuts at: 0 1042 1251
Size: 1042 209
BsiHKAI G_wGCw'C
Cuts at: 0 218 1251
Size: 218 1033
BsmBI CGTCTCn'nnnn_
Cuts at: 0 762 1251
Size: 762 489
Bsp1286I G_dGCh'C
Cuts at: 0 218 276 639 717 843 1228 1251
Size: 218 58 363 78 126 385 23
Fragments arranged by size:
385 363 218 126 78 58 23
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 14 168 650 672 1069 1251
Size: 14 154 482 22 397 182
Fragments arranged by size:
482 397 182 154 22 14
BsrBI CCG'CTC
Cuts at: 0 1008 1251
Size: 1008 243
BsrFI r'CCGG_y
Cuts at: 0 315 1251
Size: 315 936
BstYI r'GATC_y
Cuts at: 0 503 1251
Size: 503 748
BtgI C'CryG_G
Cuts at: 0 353 1251
Size: 353 898
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 324 1251
Size: 324 927
ClaI AT'CG_AT
Cuts at: 0 80 1251
Size: 80 1171
EaeI y'GGCC_r
Cuts at: 0 356 417 1251
Size: 356 61 834
Fragments arranged by size:
834 356 61
EarI CTCTTCn'nnn_
Cuts at: 0 806 1251
Size: 806 445
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 457 973 1112 1251
Size: 457 516 139 139
Fragments arranged by size:
516 457 139 139
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 320 564 1050 1251
Size: 148 172 244 486 201
Fragments arranged by size:
486 244 201 172 148
EcoNI CCTnn'n_nnAGG
Cuts at: 0 91 1251
Size: 91 1160
EcoO109I rG'GnC_Cy
Cuts at: 0 700 713 714 743 1251
Size: 700 13 1 29 508
Fragments arranged by size:
700 508 29 13 1
FspI TGC'GCA
Cuts at: 0 1024 1251
Size: 1024 227
HaeII r_GCGC'y
Cuts at: 0 24 384 618 1248 1251
Size: 24 360 234 630 3
Fragments arranged by size:
630 360 234 24 3
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 203 235 544 576 1251
Size: 203 32 309 32 675
Fragments arranged by size:
675 309 203 32 32
MscI TGG'CCA
Cuts at: 0 358 1251
Size: 358 893
MslI CAynn'nnrTG
Cuts at: 0 352 1251
Size: 352 899
MspA1I CmG'CkG
Cuts at: 0 395 1101 1134 1251
Size: 395 706 33 117
Fragments arranged by size:
706 395 117 33
NaeI GCC'GGC
Cuts at: 0 317 1251
Size: 317 934
NcoI C'CATG_G
Cuts at: 0 353 1251
Size: 353 898
NgoMIV G'CCGG_C
Cuts at: 0 315 1251
Size: 315 936
NsiI A_TGCA'T
Cuts at: 0 966 1251
Size: 966 285
NspI r_CATG'y
Cuts at: 0 573 1251
Size: 573 678
PciI A'CATG_T
Cuts at: 0 569 1251
Size: 569 682
PfoI T'CCnGG_A
Cuts at: 0 50 1251
Size: 50 1201
PspOMI G'GGCC_C
Cuts at: 0 713 1251
Size: 713 538
PstI C_TGCA'G
Cuts at: 0 179 898 1251
Size: 179 719 353
Fragments arranged by size:
719 353 179
SfcI C'TryA_G
Cuts at: 0 175 894 1251
Size: 175 719 357
Fragments arranged by size:
719 357 175
SmaI CCC'GGG
Cuts at: 0 632 740 765 1251
Size: 632 108 25 486
Fragments arranged by size:
632 486 108 25
StyI C'CwwG_G
Cuts at: 0 280 353 918 1204 1251
Size: 280 73 565 286 47
Fragments arranged by size:
565 286 280 73 47
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 539 710 1251
Size: 539 171 541
Fragments arranged by size:
541 539 171
XmaI C'CCGG_G
Cuts at: 0 630 738 763 1251
Size: 630 108 25 488
Fragments arranged by size:
630 488 108 25
XmnI GAAnn'nnTTC
Cuts at: 0 607 1251
Size: 607 644
Enzymes that do cut:
AarI AcuI AflIII AlwNI ApaI AvaI BanI BanII
BbsI BbvCI BcgI BfrBI BglI BlpI Bme1580I BpmI
Bpu10I BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspMI BsrBI BsrFI BstYI BtgI BtgZI
ClaI EaeI EarI EciI Eco57MI EcoNI EcoO109I FspI
HaeII Hin4I MscI MslI MspA1I NaeI NcoI NgoMIV
NsiI NspI PciI PfoI PspOMI PstI SfcI SmaI
StyI TaqII XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BciVI BclI BglII BmgBI
BmrI BmtI BplI BpuEI BsaI BsaAI BsaBI BsaWI
BsiWI BsmI BspEI BspHI BsrDI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtsI CspCI
DraI DraIII DrdI EagI EcoICRI EcoRI EcoRV FalI
FseI FspAI HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI NarI NdeI NheI NotI NruI PacI
PasI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI
PspXI PsrI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmlI SnaBI SpeI SphI SrfI SspI StuI
SwaI TatI TstI Tth111I XbaI XcmI XhoI ZraI