Restriction map for
pFN21AE3679
(Linear) (Six-base) MAPSORT of: KIEE3679.seq Check: 5221 from: 1 to: 912
KIEE3679.orf
With 182 enzymes: SgfI *
December 27, 2011 16:38 ..
AflIII A'CryG_T
Cuts at: 0 790 912
Size: 790 122
AgeI A'CCGG_T
Cuts at: 0 53 912
Size: 53 859
ApoI r'AATT_y
Cuts at: 0 462 912
Size: 462 450
AvaI C'yCGr_G
Cuts at: 0 30 386 912
Size: 30 356 526
Fragments arranged by size:
526 356 30
BanI G'GyrC_C
Cuts at: 0 34 912
Size: 34 878
BbeI G_GCGC'C
Cuts at: 0 38 912
Size: 38 874
BglII A'GATC_T
Cuts at: 0 382 409 912
Size: 382 27 503
Fragments arranged by size:
503 382 27
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 398 519 912
Size: 398 121 393
Fragments arranged by size:
398 393 121
BsaBI GATnn'nnATC
Cuts at: 0 123 381 912
Size: 123 258 531
Fragments arranged by size:
531 258 123
BsaHI Gr'CG_yC
Cuts at: 0 35 912
Size: 35 877
BsaWI w'CCGG_w
Cuts at: 0 53 99 912
Size: 53 46 813
Fragments arranged by size:
813 53 46
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 585 615 912
Size: 585 30 297
Fragments arranged by size:
585 297 30
BseYI C'CCAG_C
Cuts at: 0 569 606 912
Size: 569 37 306
Fragments arranged by size:
569 306 37
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 496 912
Size: 496 416
BsiEI CG_ry'CG
Cuts at: 0 20 912
Size: 20 892
BsiHKAI G_wGCw'C
Cuts at: 0 850 912
Size: 850 62
Bsp1286I G_dGCh'C
Cuts at: 0 850 912
Size: 850 62
BsrBI CCG'CTC
Cuts at: 0 503 912
Size: 503 409
BsrDI GCAATG_nn'
Cuts at: 0 885 912
Size: 885 27
BsrFI r'CCGG_y
Cuts at: 0 53 912
Size: 53 859
BstAPI GCAn_nnn'nTGC
Cuts at: 0 466 912
Size: 466 446
BstEII G'GTnAC_C
Cuts at: 0 316 912
Size: 316 596
BstYI r'GATC_y
Cuts at: 0 382 409 478 912
Size: 382 27 69 434
Fragments arranged by size:
434 382 69 27
BtgI C'CryG_G
Cuts at: 0 355 769 912
Size: 355 414 143
Fragments arranged by size:
414 355 143
EaeI y'GGCC_r
Cuts at: 0 17 912
Size: 17 895
EagI C'GGCC_G
Cuts at: 0 17 912
Size: 17 895
EarI CTCTTCn'nnn_
Cuts at: 0 638 707 802 912
Size: 638 69 95 110
Fragments arranged by size:
638 110 95 69
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 40 912
Size: 19 21 872
Fragments arranged by size:
872 21 19
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 398 519 912
Size: 398 121 393
Fragments arranged by size:
398 393 121
EcoO109I rG'GnC_Cy
Cuts at: 0 598 912
Size: 598 314
HaeII r_GCGC'y
Cuts at: 0 38 912
Size: 38 874
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 615 636 647 668 912
Size: 615 21 11 21 244
Fragments arranged by size:
615 244 21 21 11
KasI G'GCGC_C
Cuts at: 0 34 912
Size: 34 878
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 163 352 542 661 912
Size: 163 189 190 119 251
Fragments arranged by size:
251 190 189 163 119
MspA1I CmG'CkG
Cuts at: 0 40 610 912
Size: 40 570 302
Fragments arranged by size:
570 302 40
NarI GG'CG_CC
Cuts at: 0 35 912
Size: 35 877
NspI r_CATG'y
Cuts at: 0 794 912
Size: 794 118
PasI CC'CwG_GG
Cuts at: 0 523 912
Size: 523 389
PciI A'CATG_T
Cuts at: 0 790 912
Size: 790 122
PfoI T'CCnGG_A
Cuts at: 0 474 496 912
Size: 474 22 416
Fragments arranged by size:
474 416 22
PshAI GACnn'nnGTC
Cuts at: 0 791 912
Size: 791 121
PvuII CAG'CTG
Cuts at: 0 610 912
Size: 610 302
SapI GCTCTTCn'nnn_
Cuts at: 0 802 912
Size: 802 110
SfcI C'TryA_G
Cuts at: 0 41 912
Size: 41 871
SfoI GGC'GCC
Cuts at: 0 36 912
Size: 36 876
SmlI C'TyrA_G
Cuts at: 0 386 912
Size: 386 526
StuI AGG'CCT
Cuts at: 0 856 912
Size: 856 56
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 478 510 585 617 618 650 912
Size: 478 32 75 32 1 32 262
Fragments arranged by size:
478 262 75 32 32 32 1
XhoI C'TCGA_G
Cuts at: 0 386 912
Size: 386 526
Enzymes that do cut:
AflIII AgeI ApoI AvaI BanI BbeI BglII BpmI
BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BsrBI BsrDI BsrFI BstAPI BstEII BstYI BtgI
EaeI EagI EarI EciI Eco57MI EcoO109I HaeII Hin4I
KasI MmeI MspA1I NarI NspI PasI PciI PfoI
PshAI PvuII SapI SfcI SfoI SmlI StuI TstI
XhoI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AhdI AleI AlfI AloI AlwNI ApaI ApaLI AscI
AseI AsiSI AvrII BaeI BamHI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BlpI Bme1580I BmgBI
BmrI BmtI BplI Bpu10I BpuEI BsaI BsaAI BseRI
BsiWI BsmI BsmBI BspEI BspHI BspMI BsrGI BssHII
BssSI BstBI BstXI BstZ17I Bsu36I BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EcoICRI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI HincII HindIII HpaI KpnI
MfeI MluI MscI MslI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PflMI PmeI PmlI
PpiI PpuMI PsiI PspOMI PspXI PsrI PstI PvuI
RsrII SacI SacII SalI SanDI SbfI ScaI SexAI
SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StyI SwaI TaqII TatI Tth111I XbaI XcmI
XmaI XmnI ZraI