Restriction map for
pFN21AE3674
(Linear) (Six-base) MAPSORT of: KIEE3674.seq Check: 6833 from: 1 to: 1197
KIEE3674.orf
With 182 enzymes: SgfI *
December 27, 2011 16:38 ..
AccI GT'mk_AC
Cuts at: 0 743 1197
Size: 743 454
Acc65I G'GTAC_C
Cuts at: 0 626 1197
Size: 626 571
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 115 964 1197
Size: 115 849 233
Fragments arranged by size:
849 233 115
AfeI AGC'GCT
Cuts at: 0 304 1197
Size: 304 893
AgeI A'CCGG_T
Cuts at: 0 178 1197
Size: 178 1019
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 411 1197
Size: 411 786
ApoI r'AATT_y
Cuts at: 0 668 763 1197
Size: 668 95 434
Fragments arranged by size:
668 434 95
AvaI C'yCGr_G
Cuts at: 0 167 1197
Size: 167 1030
BanI G'GyrC_C
Cuts at: 0 175 626 824 832 1197
Size: 175 451 198 8 365
Fragments arranged by size:
451 365 198 175 8
BbeI G_GCGC'C
Cuts at: 0 836 1197
Size: 836 361
BglII A'GATC_T
Cuts at: 0 807 1197
Size: 807 390
BmrI ACTGGGnnnn_n'
Cuts at: 0 731 1197
Size: 731 466
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 101 1197
Size: 101 1096
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 587 1197
Size: 587 610
BsaBI GATnn'nnATC
Cuts at: 0 905 1197
Size: 905 292
BsaHI Gr'CG_yC
Cuts at: 0 833 1197
Size: 833 364
BsaWI w'CCGG_w
Cuts at: 0 178 1197
Size: 178 1019
BseYI C'CCAG_C
Cuts at: 0 191 691 1197
Size: 191 500 506
Fragments arranged by size:
506 500 191
BsmI GAATG_Cn'
Cuts at: 0 987 1197
Size: 987 210
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 187 1163 1197
Size: 187 976 34
Fragments arranged by size:
976 187 34
BsrFI r'CCGG_y
Cuts at: 0 178 1197
Size: 178 1019
BstAPI GCAn_nnn'nTGC
Cuts at: 0 202 982 1197
Size: 202 780 215
Fragments arranged by size:
780 215 202
BstYI r'GATC_y
Cuts at: 0 807 1197
Size: 807 390
BtgI C'CryG_G
Cuts at: 0 828 966 1197
Size: 828 138 231
Fragments arranged by size:
828 231 138
BtsI GCAGTG_nn'
Cuts at: 0 350 1197
Size: 350 847
DrdI GACnn_nn'nnGTC
Cuts at: 0 1159 1197
Size: 1159 38
EaeI y'GGCC_r
Cuts at: 0 50 347 1197
Size: 50 297 850
Fragments arranged by size:
850 297 50
EarI CTCTTCn'nnn_
Cuts at: 0 488 580 1197
Size: 488 92 617
Fragments arranged by size:
617 488 92
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 115 587 964 1197
Size: 115 472 377 233
Fragments arranged by size:
472 377 233 115
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1015 1197
Size: 1015 182
EcoO109I rG'GnC_Cy
Cuts at: 0 240 334 1040 1197
Size: 240 94 706 157
Fragments arranged by size:
706 240 157 94
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 609 1197
Size: 609 588
HaeII r_GCGC'y
Cuts at: 0 306 836 1197
Size: 306 530 361
Fragments arranged by size:
530 361 306
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 417 449 1197
Size: 417 32 748
Fragments arranged by size:
748 417 32
KasI G'GCGC_C
Cuts at: 0 832 1197
Size: 832 365
KpnI G_GTAC'C
Cuts at: 0 630 1197
Size: 630 567
MslI CAynn'nnrTG
Cuts at: 0 224 269 1197
Size: 224 45 928
Fragments arranged by size:
928 224 45
MspA1I CmG'CkG
Cuts at: 0 352 759 1197
Size: 352 407 438
Fragments arranged by size:
438 407 352
NarI GG'CG_CC
Cuts at: 0 833 1197
Size: 833 364
NcoI C'CATG_G
Cuts at: 0 828 1197
Size: 828 369
NspI r_CATG'y
Cuts at: 0 855 1197
Size: 855 342
PasI CC'CwG_GG
Cuts at: 0 838 1197
Size: 838 359
PpuMI rG'GwC_Cy
Cuts at: 0 240 1197
Size: 240 957
PsiI TTA'TAA
Cuts at: 0 38 1197
Size: 38 1159
PstI C_TGCA'G
Cuts at: 0 978 1197
Size: 978 219
PvuII CAG'CTG
Cuts at: 0 759 1197
Size: 759 438
SanDI GG'GwC_CC
Cuts at: 0 240 1197
Size: 240 957
SapI GCTCTTCn'nnn_
Cuts at: 0 488 1197
Size: 488 709
SfcI C'TryA_G
Cuts at: 0 253 974 1197
Size: 253 721 223
Fragments arranged by size:
721 253 223
SfoI GGC'GCC
Cuts at: 0 834 1197
Size: 834 363
SspI AAT'ATT
Cuts at: 0 637 1197
Size: 637 560
StuI AGG'CCT
Cuts at: 0 149 865 1197
Size: 149 716 332
Fragments arranged by size:
716 332 149
StyI C'CwwG_G
Cuts at: 0 708 828 1197
Size: 708 120 369
Fragments arranged by size:
708 369 120
Tth111I GACn'n_nGTC
Cuts at: 0 748 1197
Size: 748 449
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 826 835 1197
Size: 826 9 362
Fragments arranged by size:
826 362 9
XmnI GAAnn'nnTTC
Cuts at: 0 518 1197
Size: 518 679
Enzymes that do cut:
AccI Acc65I AcuI AfeI AgeI AlfI ApoI AvaI
BanI BbeI BglII BmrI BplI BpmI BsaBI BsaHI
BsaWI BseYI BsmI BspMI BsrFI BstAPI BstYI BtgI
BtsI DrdI EaeI EarI Eco57MI EcoNI EcoO109I FalI
HaeII Hin4I KasI KpnI MslI MspA1I NarI NcoI
NspI PasI PpuMI PsiI PstI PvuII SanDI SapI
SfcI SfoI SspI StuI StyI Tth111I XcmI XmnI
Enzymes that do not cut:
AarI AatII AclI AflII AflIII AhdI AleI AloI
AlwNI ApaI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BanII BbsI BbvCI BcgI BciVI BclI BfrBI
BglI BlpI Bme1580I BmgBI BmtI Bpu10I BpuEI BsaI
BsaAI BsaXI BseRI BsgI BsiEI BsiHKAI BsiWI BsmBI
Bsp1286I BspEI BspHI BsrBI BsrDI BsrGI BssHII BssSI
BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI
DraI DraIII EagI EciI EcoICRI EcoRI EcoRV FseI
FspI FspAI HincII HindIII HpaI MfeI MluI MmeI
MscI NaeI NdeI NgoMIV NheI NotI NruI NsiI
PacI PciI PflMI PfoI PmeI PmlI PpiI PshAI
PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SwaI TaqII TatI TstI
XbaI XhoI XmaI ZraI