Restriction map for
pFN21AE3669
(Linear) (Six-base) MAPSORT of: KIEE3669.seq Check: 3390 from: 1 to: 1389
KIEE3669.orf
With 182 enzymes: SgfI *
December 27, 2011 16:38 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 208 1144 1201 1389
Size: 208 936 57 188
Fragments arranged by size:
936 208 188 57
AfeI AGC'GCT
Cuts at: 0 496 1389
Size: 496 893
AflIII A'CryG_T
Cuts at: 0 755 941 971 1389
Size: 755 186 30 418
Fragments arranged by size:
755 418 186 30
AhdI GACnn_n'nnGTC
Cuts at: 0 987 1389
Size: 987 402
AleI CACnn'nnGTG
Cuts at: 0 862 1389
Size: 862 527
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1025 1389
Size: 1025 364
AvaI C'yCGr_G
Cuts at: 0 1047 1389
Size: 1047 342
BamHI G'GATC_C
Cuts at: 0 154 1389
Size: 154 1235
BanI G'GyrC_C
Cuts at: 0 783 1212 1339 1389
Size: 783 429 127 50
Fragments arranged by size:
783 429 127 50
BanII G_rGCy'C
Cuts at: 0 611 1389
Size: 611 778
BbeI G_GCGC'C
Cuts at: 0 1343 1389
Size: 1343 46
BbsI GAAGACnn'nnnn_
Cuts at: 0 19 1389
Size: 19 1370
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 198 232 1389
Size: 198 34 1157
Fragments arranged by size:
1157 198 34
BclI T'GATC_A
Cuts at: 0 305 1389
Size: 305 1084
BglI GCCn_nnn'nGGC
Cuts at: 0 415 1389
Size: 415 974
Bme1580I G_kGCm'C
Cuts at: 0 566 575 788 1389
Size: 566 9 213 601
Fragments arranged by size:
601 566 213 9
BmgBI CAC'GTC
Cuts at: 0 756 1389
Size: 756 633
BmrI ACTGGGnnnn_n'
Cuts at: 0 1242 1389
Size: 1242 147
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 920 1260 1389
Size: 920 340 129
Fragments arranged by size:
920 340 129
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 625 706 1180 1208 1389
Size: 625 81 474 28 181
Fragments arranged by size:
625 474 181 81 28
BsaI GGTCTCn'nnnn_
Cuts at: 0 235 796 1389
Size: 235 561 593
Fragments arranged by size:
593 561 235
BsaAI yAC'GTr
Cuts at: 0 45 1389
Size: 45 1344
BsaHI Gr'CG_yC
Cuts at: 0 1307 1340 1389
Size: 1307 33 49
Fragments arranged by size:
1307 49 33
BsaWI w'CCGG_w
Cuts at: 0 52 1389
Size: 52 1337
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 34 64 1389
Size: 34 30 1325
Fragments arranged by size:
1325 34 30
BseYI C'CCAG_C
Cuts at: 0 426 1389
Size: 426 963
BsiEI CG_ry'CG
Cuts at: 0 820 1266 1389
Size: 820 446 123
Fragments arranged by size:
820 446 123
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1184 1389
Size: 1184 205
Bsp1286I G_dGCh'C
Cuts at: 0 566 575 611 788 1389
Size: 566 9 36 177 601
Fragments arranged by size:
601 566 177 36 9
BspEI T'CCGG_A
Cuts at: 0 52 1389
Size: 52 1337
BsrBI CCG'CTC
Cuts at: 0 1334 1389
Size: 1334 55
BsrFI r'CCGG_y
Cuts at: 0 477 805 1063 1139 1342 1389
Size: 477 328 258 76 203 47
Fragments arranged by size:
477 328 258 203 76 47
BsrGI T'GTAC_A
Cuts at: 0 758 1389
Size: 758 631
BssSI C'ACGA_G
Cuts at: 0 886 1389
Size: 886 503
BstAPI GCAn_nnn'nTGC
Cuts at: 0 411 1389
Size: 411 978
BstEII G'GTnAC_C
Cuts at: 0 831 1350 1389
Size: 831 519 39
Fragments arranged by size:
831 519 39
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1233 1389
Size: 1233 156
BstYI r'GATC_y
Cuts at: 0 154 1389
Size: 154 1235
BtgI C'CryG_G
Cuts at: 0 68 625 722 788 1389
Size: 68 557 97 66 601
Fragments arranged by size:
601 557 97 68 66
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1086 1389
Size: 1086 303
BtsI GCAGTG_nn'
Cuts at: 0 341 1389
Size: 341 1048
ClaI AT'CG_AT
Cuts at: 0 269 1389
Size: 269 1120
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 957 992 1389
Size: 957 35 397
Fragments arranged by size:
957 397 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 508 1389
Size: 508 881
EaeI y'GGCC_r
Cuts at: 0 39 279 554 817 1196 1263 1298 1389
Size: 39 240 275 263 379 67 35 91
Fragments arranged by size:
379 275 263 240 91 67 39 35
EagI C'GGCC_G
Cuts at: 0 817 1263 1389
Size: 817 446 126
Fragments arranged by size:
817 446 126
EarI CTCTTCn'nnn_
Cuts at: 0 1324 1389
Size: 1324 65
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 208 340 1389
Size: 208 132 1049
Fragments arranged by size:
1049 208 132
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 208 625 706 1144 1180 1201 1208 1389
Size: 208 417 81 438 36 21 7 181
Fragments arranged by size:
438 417 208 181 81 36 21 7
EcoNI CCTnn'n_nnAGG
Cuts at: 0 685 1389
Size: 685 704
EcoO109I rG'GnC_Cy
Cuts at: 0 690 1389
Size: 690 699
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 174 1389
Size: 174 1215
FspI TGC'GCA
Cuts at: 0 333 1389
Size: 333 1056
HaeII r_GCGC'y
Cuts at: 0 498 527 1064 1146 1343 1389
Size: 498 29 537 82 197 46
Fragments arranged by size:
537 498 197 82 46 29
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 853 885 973 1005 1129 1161 1389
Size: 853 32 88 32 124 32 228
Fragments arranged by size:
853 228 124 88 32 32 32
KasI G'GCGC_C
Cuts at: 0 1339 1389
Size: 1339 50
MslI CAynn'nnrTG
Cuts at: 0 829 862 1231 1389
Size: 829 33 369 158
Fragments arranged by size:
829 369 158 33
MspA1I CmG'CkG
Cuts at: 0 747 1389
Size: 747 642
NaeI GCC'GGC
Cuts at: 0 479 1065 1344 1389
Size: 479 586 279 45
Fragments arranged by size:
586 479 279 45
NarI GG'CG_CC
Cuts at: 0 1340 1389
Size: 1340 49
NgoMIV G'CCGG_C
Cuts at: 0 477 1063 1342 1389
Size: 477 586 279 47
Fragments arranged by size:
586 477 279 47
NspI r_CATG'y
Cuts at: 0 409 603 624 1389
Size: 409 194 21 765
Fragments arranged by size:
765 409 194 21
PmlI CAC'GTG
Cuts at: 0 45 1389
Size: 45 1344
PstI C_TGCA'G
Cuts at: 0 752 1389
Size: 752 637
SapI GCTCTTCn'nnn_
Cuts at: 0 1324 1389
Size: 1324 65
ScaI AGT'ACT
Cuts at: 0 253 1389
Size: 253 1136
SfcI C'TryA_G
Cuts at: 0 483 748 1389
Size: 483 265 641
Fragments arranged by size:
641 483 265
SfoI GGC'GCC
Cuts at: 0 1341 1389
Size: 1341 48
SmaI CCC'GGG
Cuts at: 0 1049 1389
Size: 1049 340
SphI G_CATG'C
Cuts at: 0 409 1389
Size: 409 980
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 162 418 1389
Size: 162 256 971
Fragments arranged by size:
971 256 162
TatI w'GTAC_w
Cuts at: 0 251 758 1389
Size: 251 507 631
Fragments arranged by size:
631 507 251
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 34 66 1389
Size: 34 32 1323
Fragments arranged by size:
1323 34 32
Tth111I GACn'n_nGTC
Cuts at: 0 919 1389
Size: 919 470
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1206 1389
Size: 1206 183
XmaI C'CCGG_G
Cuts at: 0 1047 1389
Size: 1047 342
Enzymes that do cut:
AcuI AfeI AflIII AhdI AleI AlfI AvaI BamHI
BanI BanII BbeI BbsI BcgI BclI BglI Bme1580I
BmgBI BmrI BplI BpmI BsaI BsaAI BsaHI BsaWI
BsaXI BseYI BsiEI BsmBI Bsp1286I BspEI BsrBI BsrFI
BsrGI BssSI BstAPI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I FalI FspI HaeII Hin4I KasI
MslI MspA1I NaeI NarI NgoMIV NspI PmlI PstI
SapI ScaI SfcI SfoI SmaI SphI TaqII TatI
TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AgeI AloI
AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BbvCI BciVI BfrBI BglII BlpI BmtI Bpu10I
BpuEI BsaBI BseRI BsgI BsiHKAI BsiWI BsmI BspHI
BspMI BsrDI BssHII BstBI BstZ17I Bsu36I DraI DraIII
EcoICRI EcoRI EcoRV FseI FspAI HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PvuI PvuII RsrII SacI SacII SalI SanDI SbfI
SexAI SfiI SgfI SgrAI SmlI SnaBI SpeI SrfI
SspI StuI StyI SwaI XbaI XhoI XmnI ZraI