Restriction map for
pFN21AE3668
(Linear) (Six-base) MAPSORT of: KIEE3668.seq Check: 6106 from: 1 to: 1395
KIEE3668.orf
With 182 enzymes: SgfI *
December 27, 2011 16:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 999 1395
Size: 999 396
AccI GT'mk_AC
Cuts at: 0 1123 1395
Size: 1123 272
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 109 234 1395
Size: 109 125 1161
Fragments arranged by size:
1161 125 109
AflIII A'CryG_T
Cuts at: 0 1139 1395
Size: 1139 256
AleI CACnn'nnGTG
Cuts at: 0 181 1395
Size: 181 1214
AlwNI CAG_nnn'CTG
Cuts at: 0 57 861 1395
Size: 57 804 534
Fragments arranged by size:
804 534 57
AvaI C'yCGr_G
Cuts at: 0 331 1395
Size: 331 1064
BanI G'GyrC_C
Cuts at: 0 772 1395
Size: 772 623
BanII G_rGCy'C
Cuts at: 0 841 1395
Size: 841 554
BbeI G_GCGC'C
Cuts at: 0 776 1395
Size: 776 619
BbsI GAAGACnn'nnnn_
Cuts at: 0 310 476 653 1395
Size: 310 166 177 742
Fragments arranged by size:
742 310 177 166
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 823 857 1395
Size: 823 34 538
Fragments arranged by size:
823 538 34
BglII A'GATC_T
Cuts at: 0 1040 1395
Size: 1040 355
Bme1580I G_kGCm'C
Cuts at: 0 682 1395
Size: 682 713
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 405 1395
Size: 405 990
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 160 832 1395
Size: 160 672 563
Fragments arranged by size:
672 563 160
Bpu10I CC'TnA_GC
Cuts at: 0 919 1054 1395
Size: 919 135 341
Fragments arranged by size:
919 341 135
BsaI GGTCTCn'nnnn_
Cuts at: 0 136 1359 1395
Size: 136 1223 36
Fragments arranged by size:
1223 136 36
BsaAI yAC'GTr
Cuts at: 0 183 1395
Size: 183 1212
BsaHI Gr'CG_yC
Cuts at: 0 338 773 1163 1291 1395
Size: 338 435 390 128 104
Fragments arranged by size:
435 390 338 128 104
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 172 202 208 238 277 307 1395
Size: 172 30 6 30 39 30 1088
Fragments arranged by size:
1088 172 39 30 30 30 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 94 298 721 1339 1395
Size: 94 204 423 618 56
Fragments arranged by size:
618 423 204 94 56
BseYI C'CCAG_C
Cuts at: 0 1062 1249 1294 1386 1395
Size: 1062 187 45 92 9
Fragments arranged by size:
1062 187 92 45 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1228 1395
Size: 1228 167
BsiEI CG_ry'CG
Cuts at: 0 1032 1395
Size: 1032 363
BsiHKAI G_wGCw'C
Cuts at: 0 841 1060 1147 1395
Size: 841 219 87 248
Fragments arranged by size:
841 248 219 87
BsmBI CGTCTCn'nnnn_
Cuts at: 0 96 551 985 1395
Size: 96 455 434 410
Fragments arranged by size:
455 434 410 96
Bsp1286I G_dGCh'C
Cuts at: 0 682 841 1060 1147 1395
Size: 682 159 219 87 248
Fragments arranged by size:
682 248 219 159 87
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 947 999 1395
Size: 947 52 396
Fragments arranged by size:
947 396 52
BsrBI CCG'CTC
Cuts at: 0 1263 1395
Size: 1263 132
BsrDI GCAATG_nn'
Cuts at: 0 608 696 1395
Size: 608 88 699
Fragments arranged by size:
699 608 88
BsrFI r'CCGG_y
Cuts at: 0 346 1395
Size: 346 1049
BsrGI T'GTAC_A
Cuts at: 0 1235 1395
Size: 1235 160
BssHII G'CGCG_C
Cuts at: 0 1128 1395
Size: 1128 267
BssSI C'ACGA_G
Cuts at: 0 925 1395
Size: 925 470
BstAPI GCAn_nnn'nTGC
Cuts at: 0 686 695 1395
Size: 686 9 700
Fragments arranged by size:
700 686 9
BstYI r'GATC_y
Cuts at: 0 53 1040 1395
Size: 53 987 355
Fragments arranged by size:
987 355 53
Bsu36I CC'TnA_GG
Cuts at: 0 133 519 1395
Size: 133 386 876
Fragments arranged by size:
876 386 133
BtgI C'CryG_G
Cuts at: 0 126 948 1395
Size: 126 822 447
Fragments arranged by size:
822 447 126
BtsI GCAGTG_nn'
Cuts at: 0 298 350 1338 1395
Size: 298 52 988 57
Fragments arranged by size:
988 298 57 52
ClaI AT'CG_AT
Cuts at: 0 476 1395
Size: 476 919
EaeI y'GGCC_r
Cuts at: 0 620 1335 1395
Size: 620 715 60
Fragments arranged by size:
715 620 60
EarI CTCTTCn'nnn_
Cuts at: 0 146 370 949 1395
Size: 146 224 579 446
Fragments arranged by size:
579 446 224 146
EcoICRI GAG'CTC
Cuts at: 0 839 1395
Size: 839 556
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 109 160 234 832 1395
Size: 109 51 74 598 563
Fragments arranged by size:
598 563 109 74 51
EcoNI CCTnn'n_nnAGG
Cuts at: 0 91 1395
Size: 91 1304
EcoO109I rG'GnC_Cy
Cuts at: 0 84 1395
Size: 84 1311
FspI TGC'GCA
Cuts at: 0 973 1136 1207 1282 1395
Size: 973 163 71 75 113
Fragments arranged by size:
973 163 113 75 71
FspAI rTGC'GCAy
Cuts at: 0 1207 1395
Size: 1207 188
HaeII r_GCGC'y
Cuts at: 0 776 1395
Size: 776 619
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 208 240 361 393 548 580 823 855
Size: 208 32 121 32 155 32 243 32
Cuts at: 855 1105 1137 1395
Size: 250 32 258
Fragments arranged by size:
258 250 243 208 155 121 32 32
32 32 32
KasI G'GCGC_C
Cuts at: 0 772 1395
Size: 772 623
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 416 1301 1395
Size: 416 885 94
Fragments arranged by size:
885 416 94
MscI TGG'CCA
Cuts at: 0 622 1395
Size: 622 773
MslI CAynn'nnrTG
Cuts at: 0 181 673 1213 1395
Size: 181 492 540 182
Fragments arranged by size:
540 492 182 181
MspA1I CmG'CkG
Cuts at: 0 72 128 861 969 1298 1395
Size: 72 56 733 108 329 97
Fragments arranged by size:
733 329 108 97 72 56
NarI GG'CG_CC
Cuts at: 0 773 1395
Size: 773 622
NspI r_CATG'y
Cuts at: 0 753 1143 1179 1395
Size: 753 390 36 216
Fragments arranged by size:
753 390 216 36
PasI CC'CwG_GG
Cuts at: 0 435 1395
Size: 435 960
PciI A'CATG_T
Cuts at: 0 1139 1395
Size: 1139 256
PfoI T'CCnGG_A
Cuts at: 0 398 1395
Size: 398 997
PpuMI rG'GwC_Cy
Cuts at: 0 84 1395
Size: 84 1311
PstI C_TGCA'G
Cuts at: 0 77 1395
Size: 77 1318
PvuII CAG'CTG
Cuts at: 0 72 861 969 1298 1395
Size: 72 789 108 329 97
Fragments arranged by size:
789 329 108 97 72
SacI G_AGCT'C
Cuts at: 0 841 1395
Size: 841 554
SacII CC_GC'GG
Cuts at: 0 129 1395
Size: 129 1266
SapI GCTCTTCn'nnn_
Cuts at: 0 370 949 1395
Size: 370 579 446
Fragments arranged by size:
579 446 370
SfcI C'TryA_G
Cuts at: 0 73 1395
Size: 73 1322
SfoI GGC'GCC
Cuts at: 0 774 1395
Size: 774 621
SphI G_CATG'C
Cuts at: 0 1179 1395
Size: 1179 216
StuI AGG'CCT
Cuts at: 0 517 958 1378 1395
Size: 517 441 420 17
Fragments arranged by size:
517 441 420 17
StyI C'CwwG_G
Cuts at: 0 503 1395
Size: 503 892
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 226 1395
Size: 226 1169
TatI w'GTAC_w
Cuts at: 0 320 887 1235 1395
Size: 320 567 348 160
Fragments arranged by size:
567 348 320 160
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 277 309 1395
Size: 277 32 1086
Fragments arranged by size:
1086 277 32
Enzymes that do cut:
AarI AccI AcuI AflIII AleI AlwNI AvaI BanI
BanII BbeI BbsI BcgI BglII Bme1580I BplI BpmI
Bpu10I BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstYI Bsu36I BtgI BtsI
ClaI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I FspI
FspAI HaeII Hin4I KasI MmeI MscI MslI MspA1I
NarI NspI PasI PciI PfoI PpuMI PstI PvuII
SacI SacII SapI SfcI SfoI SphI StuI StyI
TaqII TatI TstI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AlfI
AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbvCI BciVI BclI BfrBI BglI BlpI
BmgBI BmrI BmtI BpuEI BsaBI BsaWI BsiWI BsmI
BspEI BspHI BstBI BstEII BstXI BstZ17I BtgZI CspCI
DraI DraIII DrdI EagI EciI EcoRI EcoRV FalI
FseI HincII HindIII HpaI KpnI MfeI MluI NaeI
NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI
PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SalI SanDI SbfI ScaI SexAI
SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI
SspI SwaI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI