Restriction map for
pFN21AE3666
(Linear) (Six-base) MAPSORT of: KIEE3666.seq Check: 2583 from: 1 to: 1065
KIEE3666.orf
With 182 enzymes: SgfI *
December 27, 2011 16:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 360 1065
Size: 360 705
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 937 1065
Size: 937 128
AflII C'TTAA_G
Cuts at: 0 106 1065
Size: 106 959
AflIII A'CryG_T
Cuts at: 0 973 1065
Size: 973 92
AlwNI CAG_nnn'CTG
Cuts at: 0 702 1065
Size: 702 363
ApoI r'AATT_y
Cuts at: 0 783 1051 1065
Size: 783 268 14
Fragments arranged by size:
783 268 14
BbsI GAAGACnn'nnnn_
Cuts at: 0 203 1065
Size: 203 862
BciVI GTATCCnnnnn_n'
Cuts at: 0 275 572 1065
Size: 275 297 493
Fragments arranged by size:
493 297 275
BclI T'GATC_A
Cuts at: 0 257 1065
Size: 257 808
Bme1580I G_kGCm'C
Cuts at: 0 542 1065
Size: 542 523
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 345 1065
Size: 345 720
Bpu10I CC'TnA_GC
Cuts at: 0 277 1065
Size: 277 788
BsaI GGTCTCn'nnnn_
Cuts at: 0 354 1065
Size: 354 711
BsaBI GATnn'nnATC
Cuts at: 0 935 1065
Size: 935 130
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 22 52 334 364 1065
Size: 22 30 282 30 701
Fragments arranged by size:
701 282 30 30 22
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 43 444 1065
Size: 43 401 621
Fragments arranged by size:
621 401 43
BseYI C'CCAG_C
Cuts at: 0 120 1065
Size: 120 945
BsiEI CG_ry'CG
Cuts at: 0 619 1065
Size: 619 446
Bsp1286I G_dGCh'C
Cuts at: 0 542 1065
Size: 542 523
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 360 1065
Size: 360 705
BsrFI r'CCGG_y
Cuts at: 0 4 547 1065
Size: 4 543 518
Fragments arranged by size:
543 518 4
BssHII G'CGCG_C
Cuts at: 0 94 1065
Size: 94 971
BstXI CCAn_nnnn'nTGG
Cuts at: 0 181 1065
Size: 181 884
BstYI r'GATC_y
Cuts at: 0 399 816 1065
Size: 399 417 249
Fragments arranged by size:
417 399 249
Bsu36I CC'TnA_GG
Cuts at: 0 1045 1065
Size: 1045 20
BtgI C'CryG_G
Cuts at: 0 23 174 1065
Size: 23 151 891
Fragments arranged by size:
891 151 23
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 280 471 1065
Size: 280 191 594
Fragments arranged by size:
594 280 191
BtsI GCAGTG_nn'
Cuts at: 0 639 1065
Size: 639 426
DraI TTT'AAA
Cuts at: 0 753 1065
Size: 753 312
DrdI GACnn_nn'nnGTC
Cuts at: 0 74 1065
Size: 74 991
EaeI y'GGCC_r
Cuts at: 0 2 609 1065
Size: 2 607 456
Fragments arranged by size:
607 456 2
EarI CTCTTCn'nnn_
Cuts at: 0 728 821 1065
Size: 728 93 244
Fragments arranged by size:
728 244 93
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 345 937 1065
Size: 345 592 128
Fragments arranged by size:
592 345 128
EcoO109I rG'GnC_Cy
Cuts at: 0 699 1065
Size: 699 366
EcoRI G'AATT_C
Cuts at: 0 783 1051 1065
Size: 783 268 14
Fragments arranged by size:
783 268 14
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 541 1065
Size: 541 524
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 22 54 334 366 896 928 1065
Size: 22 32 280 32 530 32 137
Fragments arranged by size:
530 280 137 32 32 32 22
HindIII A'AGCT_T
Cuts at: 0 919 1065
Size: 919 146
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 428 1065
Size: 428 637
MscI TGG'CCA
Cuts at: 0 611 1065
Size: 611 454
MslI CAynn'nnrTG
Cuts at: 0 244 1065
Size: 244 821
MspA1I CmG'CkG
Cuts at: 0 25 444 1065
Size: 25 419 621
Fragments arranged by size:
621 419 25
NaeI GCC'GGC
Cuts at: 0 6 1065
Size: 6 1059
NcoI C'CATG_G
Cuts at: 0 174 1065
Size: 174 891
NgoMIV G'CCGG_C
Cuts at: 0 4 1065
Size: 4 1061
NspI r_CATG'y
Cuts at: 0 977 1065
Size: 977 88
PciI A'CATG_T
Cuts at: 0 973 1065
Size: 973 92
PflMI CCAn_nnn'nTGG
Cuts at: 0 702 1065
Size: 702 363
PfoI T'CCnGG_A
Cuts at: 0 410 1065
Size: 410 655
PpuMI rG'GwC_Cy
Cuts at: 0 699 1065
Size: 699 366
PvuI CG_AT'CG
Cuts at: 0 619 1065
Size: 619 446
PvuII CAG'CTG
Cuts at: 0 444 1065
Size: 444 621
SacII CC_GC'GG
Cuts at: 0 26 1065
Size: 26 1039
SexAI A'CCwGG_T
Cuts at: 0 695 1065
Size: 695 370
SfcI C'TryA_G
Cuts at: 0 980 1033 1065
Size: 980 53 32
Fragments arranged by size:
980 53 32
SmlI C'TyrA_G
Cuts at: 0 106 1065
Size: 106 959
SspI AAT'ATT
Cuts at: 0 463 996 1065
Size: 463 533 69
Fragments arranged by size:
533 463 69
StyI C'CwwG_G
Cuts at: 0 174 1065
Size: 174 891
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 894 1065
Size: 894 171
TatI w'GTAC_w
Cuts at: 0 839 1065
Size: 839 226
Tth111I GACn'n_nGTC
Cuts at: 0 346 697 1065
Size: 346 351 368
Fragments arranged by size:
368 351 346
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 369 669 1065
Size: 369 300 396
Fragments arranged by size:
396 369 300
Enzymes that do cut:
AarI AcuI AflII AflIII AlwNI ApoI BbsI BciVI
BclI Bme1580I BpmI Bpu10I BsaI BsaBI BsaXI BseRI
BseYI BsiEI Bsp1286I BspMI BsrFI BssHII BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EarI
Eco57MI EcoO109I EcoRI FalI Hin4I HindIII MmeI MscI
MslI MspA1I NaeI NcoI NgoMIV NspI PciI PflMI
PfoI PpuMI PvuI PvuII SacII SexAI SfcI SmlI
SspI StyI TaqII TatI Tth111I XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AgeI AhdI AleI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BanI BanII BbeI BbvCI BcgI
BfrBI BglI BglII BlpI BmgBI BmrI BmtI BplI
BpuEI BsaAI BsaHI BsaWI BsgI BsiHKAI BsiWI BsmI
BsmBI BspEI BspHI BsrBI BsrDI BsrGI BssSI BstAPI
BstBI BstEII BstZ17I ClaI CspCI DraIII EagI EciI
EcoICRI EcoNI EcoRV FseI FspI FspAI HaeII HincII
HpaI KasI KpnI MfeI MluI NarI NdeI NheI
NotI NruI NsiI PacI PasI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PsrI PstI RsrII SacI
SalI SanDI SapI SbfI ScaI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI StuI SwaI
TstI XbaI XhoI XmaI XmnI ZraI