Restriction map for
pFN21AE3661
(Linear) (Six-base) MAPSORT of: KIEE3661.seq Check: 8200 from: 1 to: 1263
KIEE3661.orf
With 182 enzymes: SgfI *
December 27, 2011 16:37 ..
AccI GT'mk_AC
Cuts at: 0 1100 1263
Size: 1100 163
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 306 817 847 1097 1263
Size: 306 511 30 250 166
Fragments arranged by size:
511 306 250 166 30
AlwNI CAG_nnn'CTG
Cuts at: 0 555 1263
Size: 555 708
ApaI G_GGCC'C
Cuts at: 0 1030 1263
Size: 1030 233
ApaLI G'TGCA_C
Cuts at: 0 637 1263
Size: 637 626
AvaI C'yCGr_G
Cuts at: 0 674 1263
Size: 674 589
BanI G'GyrC_C
Cuts at: 0 192 976 1263
Size: 192 784 287
Fragments arranged by size:
784 287 192
BanII G_rGCy'C
Cuts at: 0 185 880 970 1030 1086 1263
Size: 185 695 90 60 56 177
Fragments arranged by size:
695 185 177 90 60 56
BbsI GAAGACnn'nnnn_
Cuts at: 0 312 593 622 1263
Size: 312 281 29 641
Fragments arranged by size:
641 312 281 29
BciVI GTATCCnnnnn_n'
Cuts at: 0 471 1263
Size: 471 792
BfrBI ATG'CAT
Cuts at: 0 486 1263
Size: 486 777
BglII A'GATC_T
Cuts at: 0 1196 1263
Size: 1196 67
BlpI GC'TnA_GC
Cuts at: 0 184 1226 1263
Size: 184 1042 37
Fragments arranged by size:
1042 184 37
Bme1580I G_kGCm'C
Cuts at: 0 641 1030 1263
Size: 641 389 233
Fragments arranged by size:
641 389 233
BmrI ACTGGGnnnn_n'
Cuts at: 0 278 543 1263
Size: 278 265 720
Fragments arranged by size:
720 278 265
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 808 1263
Size: 808 455
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 640 1263
Size: 640 623
BsaHI Gr'CG_yC
Cuts at: 0 4 1263
Size: 4 1259
BsaWI w'CCGG_w
Cuts at: 0 1067 1212 1263
Size: 1067 145 51
Fragments arranged by size:
1067 145 51
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 901 931 936 966 1263
Size: 901 30 5 30 297
Fragments arranged by size:
901 297 30 30 5
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 784 904 1263
Size: 784 120 359
Fragments arranged by size:
784 359 120
BseYI C'CCAG_C
Cuts at: 0 391 1186 1263
Size: 391 795 77
Fragments arranged by size:
795 391 77
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 121 841 1263
Size: 121 720 422
Fragments arranged by size:
720 422 121
BsiEI CG_ry'CG
Cuts at: 0 131 438 1263
Size: 131 307 825
Fragments arranged by size:
825 307 131
BsiHKAI G_wGCw'C
Cuts at: 0 185 641 1263
Size: 185 456 622
Fragments arranged by size:
622 456 185
Bsp1286I G_dGCh'C
Cuts at: 0 185 641 880 970 1030 1086 1263
Size: 185 456 239 90 60 56 177
Fragments arranged by size:
456 239 185 177 90 60 56
BspEI T'CCGG_A
Cuts at: 0 1067 1212 1263
Size: 1067 145 51
Fragments arranged by size:
1067 145 51
BsrDI GCAATG_nn'
Cuts at: 0 473 1263
Size: 473 790
BstAPI GCAn_nnn'nTGC
Cuts at: 0 99 1159 1263
Size: 99 1060 104
Fragments arranged by size:
1060 104 99
BstYI r'GATC_y
Cuts at: 0 1196 1263
Size: 1196 67
Bsu36I CC'TnA_GG
Cuts at: 0 718 1263
Size: 718 545
BtgI C'CryG_G
Cuts at: 0 26 528 1263
Size: 26 502 735
Fragments arranged by size:
735 502 26
BtsI GCAGTG_nn'
Cuts at: 0 414 1263
Size: 414 849
ClaI AT'CG_AT
Cuts at: 0 339 1263
Size: 339 924
EaeI y'GGCC_r
Cuts at: 0 128 1263
Size: 128 1135
EagI C'GGCC_G
Cuts at: 0 128 1263
Size: 128 1135
EarI CTCTTCn'nnn_
Cuts at: 0 761 793 1263
Size: 761 32 470
Fragments arranged by size:
761 470 32
EcoICRI GAG'CTC
Cuts at: 0 183 1263
Size: 183 1080
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 306 808 817 847 1097 1263
Size: 306 502 9 30 250 166
Fragments arranged by size:
502 306 250 166 30 9
EcoO109I rG'GnC_Cy
Cuts at: 0 49 1263
Size: 49 1214
EcoRV GAT'ATC
Cuts at: 0 1122 1263
Size: 1122 141
FspI TGC'GCA
Cuts at: 0 610 1263
Size: 610 653
HaeII r_GCGC'y
Cuts at: 0 901 1263
Size: 901 362
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 901 933 936 968 989 1021 1027 1059
Size: 901 32 3 32 21 32 6 32
Cuts at: 1059 1263
Size: 204
Fragments arranged by size:
901 204 32 32 32 32 21 6
3
HincII GTy'rAC
Cuts at: 0 252 1263
Size: 252 1011
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 524 1263
Size: 524 739
MslI CAynn'nnrTG
Cuts at: 0 601 1263
Size: 601 662
MspA1I CmG'CkG
Cuts at: 0 138 825 1263
Size: 138 687 438
Fragments arranged by size:
687 438 138
NcoI C'CATG_G
Cuts at: 0 528 1263
Size: 528 735
NsiI A_TGCA'T
Cuts at: 0 488 1263
Size: 488 775
NspI r_CATG'y
Cuts at: 0 486 600 1263
Size: 486 114 663
Fragments arranged by size:
663 486 114
PasI CC'CwG_GG
Cuts at: 0 1023 1263
Size: 1023 240
PflMI CCAn_nnn'nTGG
Cuts at: 0 555 1263
Size: 555 708
PfoI T'CCnGG_A
Cuts at: 0 1235 1263
Size: 1235 28
PshAI GACnn'nnGTC
Cuts at: 0 445 1263
Size: 445 818
PspOMI G'GGCC_C
Cuts at: 0 1026 1263
Size: 1026 237
PstI C_TGCA'G
Cuts at: 0 940 1263
Size: 940 323
PvuI CG_AT'CG
Cuts at: 0 438 1263
Size: 438 825
PvuII CAG'CTG
Cuts at: 0 825 1263
Size: 825 438
SacI G_AGCT'C
Cuts at: 0 185 1263
Size: 185 1078
SfcI C'TryA_G
Cuts at: 0 840 936 1263
Size: 840 96 327
Fragments arranged by size:
840 327 96
SmaI CCC'GGG
Cuts at: 0 676 1263
Size: 676 587
SmlI C'TyrA_G
Cuts at: 0 655 1263
Size: 655 608
SspI AAT'ATT
Cuts at: 0 782 1263
Size: 782 481
StyI C'CwwG_G
Cuts at: 0 528 1263
Size: 528 735
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 3 133 1263
Size: 3 130 1130
Fragments arranged by size:
1130 130 3
TatI w'GTAC_w
Cuts at: 0 512 1263
Size: 512 751
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 318 350 1263
Size: 318 32 913
Fragments arranged by size:
913 318 32
XmaI C'CCGG_G
Cuts at: 0 674 1263
Size: 674 589
XmnI GAAnn'nnTTC
Cuts at: 0 94 118 1263
Size: 94 24 1145
Fragments arranged by size:
1145 94 24
Enzymes that do cut:
AccI AcuI AlwNI ApaI ApaLI AvaI BanI BanII
BbsI BciVI BfrBI BglII BlpI Bme1580I BmrI BpmI
BpuEI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BsrDI BstAPI BstYI Bsu36I BtgI
BtsI ClaI EaeI EagI EarI EcoICRI Eco57MI EcoO109I
EcoRV FspI HaeII Hin4I HincII MmeI MslI MspA1I
NcoI NsiI NspI PasI PflMI PfoI PshAI PspOMI
PstI PvuI PvuII SacI SfcI SmaI SmlI SspI
StyI TaqII TatI TstI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BbvCI BcgI BclI BglI
BmgBI BmtI BplI Bpu10I BsaI BsaAI BsaBI BsiWI
BsmI BsmBI BspHI BspMI BsrBI BsrFI BsrGI BssHII
BssSI BstBI BstEII BstXI BstZ17I BtgZI CspCI DraI
DraIII DrdI EciI EcoNI EcoRI FalI FseI FspAI
HindIII HpaI KasI KpnI MfeI MluI MscI NaeI
NarI NdeI NgoMIV NheI NotI NruI PacI PciI
PmeI PmlI PpiI PpuMI PsiI PspXI PsrI RsrII
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SnaBI SpeI SphI SrfI StuI
SwaI Tth111I XbaI XcmI XhoI ZraI