(Linear) (Six-base) MAPSORT of: KIEE3658.seq  Check: 9247  from: 1  to: 492



KIEE3658.orf

 With 182 enzymes: SgfI * 

                        December 27, 2011 16:37  ..

AlwNI CAG_nnn'CTG

Cuts at:      0     335     492 
   Size:        335     157
 
ApaI G_GGCC'C

Cuts at:      0      75     492 
   Size:         75     417
 
AvaI C'yCGr_G

Cuts at:      0     127     492 
   Size:        127     365
 
BanII G_rGCy'C

Cuts at:      0      23      75     492 
   Size:         23      52     417

  Fragments arranged by size: 

                 417      52      23
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     141     492 
   Size:        141     351
 
BclI T'GATC_A

Cuts at:      0     272     492 
   Size:        272     220
 
Bme1580I G_kGCm'C

Cuts at:      0      75     492 
   Size:         75     417
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      78     492 
   Size:         78     414
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      50     126     492 
   Size:         50      76     366

  Fragments arranged by size: 

                 366      76      50
 
Bpu10I CC'TnA_GC

Cuts at:      0     160     492 
   Size:        160     332
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      26     492 
   Size:         26     466
 
BsaAI yAC'GTr

Cuts at:      0      96     492 
   Size:         96     396
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     101     129     492 
   Size:        101      28     363

  Fragments arranged by size: 

                 363     101      28
 
BseYI C'CCAG_C

Cuts at:      0     353     492 
   Size:        353     139
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     191     492 
   Size:        191     301
 
BsiHKAI G_wGCw'C

Cuts at:      0      68     344     492 
   Size:         68     276     148

  Fragments arranged by size: 

                 276     148      68
 
Bsp1286I G_dGCh'C

Cuts at:      0      23      68      75     344     492 
   Size:         23      45       7     269     148

  Fragments arranged by size: 

                 269     148      45      23       7
 
BtgI C'CryG_G

Cuts at:      0     256     263     419     492 
   Size:        256       7     156      73

  Fragments arranged by size: 

                 256     156      73       7
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     408     443     492 
   Size:        408      35      49

  Fragments arranged by size: 

                 408      49      35
 
EaeI y'GGCC_r

Cuts at:      0     416     492 
   Size:        416      76
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      40     492 
   Size:         40     452
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      50     126     492 
   Size:         50      76     366

  Fragments arranged by size: 

                 366      76      50
 
EcoO109I rG'GnC_Cy

Cuts at:      0      72     492 
   Size:         72     420
 
HaeII r_GCGC'y

Cuts at:      0     192     427     492 
   Size:        192     235      65

  Fragments arranged by size: 

                 235     192      65
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     285     317     492 
   Size:        285      32     175

  Fragments arranged by size: 

                 285     175      32
 
HincII GTy'rAC

Cuts at:      0     228     492 
   Size:        228     264
 
MscI TGG'CCA

Cuts at:      0     418     492 
   Size:        418      74
 
MspA1I CmG'CkG

Cuts at:      0     357     377     492 
   Size:        357      20     115

  Fragments arranged by size: 

                 357     115      20
 
PspOMI G'GGCC_C

Cuts at:      0      71     492 
   Size:         71     421
 
PvuII CAG'CTG

Cuts at:      0     357     492 
   Size:        357     135
 
SmaI CCC'GGG

Cuts at:      0     129     492 
   Size:        129     363
 
StyI C'CwwG_G

Cuts at:      0      54     398     492 
   Size:         54     344      94

  Fragments arranged by size: 

                 344      94      54
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     367     492 
   Size:        367     125
 
TatI w'GTAC_w

Cuts at:      0     314     492 
   Size:        314     178
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     361     492 
   Size:        361     131
 
XmaI C'CCGG_G

Cuts at:      0     127     492 
   Size:        127     365
 

 Enzymes that do cut:

    AlwNI     ApaI     AvaI    BanII     BbsI     BclI Bme1580I     BmrI
     BpmI   Bpu10I     BsaI    BsaAI    BseRI    BseYI     BsgI  BsiHKAI
 Bsp1286I     BtgI    CspCI     EaeI     EciI  Eco57MI EcoO109I    HaeII
    Hin4I   HincII     MscI   MspA1I   PspOMI    PvuII     SmaI     StyI
    TaqII     TatI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    ApaLI     ApoI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI
    BbvCI     BcgI    BciVI    BfrBI     BglI    BglII     BlpI    BmgBI
     BmtI     BplI    BpuEI    BsaBI    BsaHI    BsaWI    BsaXI    BsiEI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
    BstYI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI     DraI   DraIII
     DrdI     EagI     EarI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI     TstI  Tth111I     XbaI     XhoI     XmnI     ZraI