Restriction map for
pFN21AE3655
(Linear) (Six-base) MAPSORT of: KIEE3655.seq Check: 9066 from: 1 to: 3036
KIEE3655.orf
With 182 enzymes: SgfI *
December 27, 2011 16:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 81 208 1051 2906 2955 3036
Size: 81 127 843 1855 49 81
Fragments arranged by size:
1855 843 127 81 81 49
AccI GT'mk_AC
Cuts at: 0 1467 3036
Size: 1467 1569
Acc65I G'GTAC_C
Cuts at: 0 2754 3036
Size: 2754 282
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 418 782 1006 1138 1431 1687 1975 2281
Size: 418 364 224 132 293 256 288 306
Cuts at: 2281 2428 2746 2770 2788 3036
Size: 147 318 24 18 248
Fragments arranged by size:
418 364 318 306 293 288 256 248
224 147 132 24 18
AfeI AGC'GCT
Cuts at: 0 1420 3036
Size: 1420 1616
AflIII A'CryG_T
Cuts at: 0 1728 3036
Size: 1728 1308
AgeI A'CCGG_T
Cuts at: 0 2505 3036
Size: 2505 531
AleI CACnn'nnGTG
Cuts at: 0 323 3036
Size: 323 2713
AlwNI CAG_nnn'CTG
Cuts at: 0 126 952 1102 1495 1771 2064 2575 2898
Size: 126 826 150 393 276 293 511 323
Cuts at: 2898 2931 3036
Size: 33 105
Fragments arranged by size:
826 511 393 323 293 276 150 126
105 33
ApaI G_GGCC'C
Cuts at: 0 1845 2401 3036
Size: 1845 556 635
Fragments arranged by size:
1845 635 556
ApaLI G'TGCA_C
Cuts at: 0 316 884 3036
Size: 316 568 2152
Fragments arranged by size:
2152 568 316
AvaI C'yCGr_G
Cuts at: 0 452 633 968 1499 3036
Size: 452 181 335 531 1537
Fragments arranged by size:
1537 531 452 335 181
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 729 762 3036
Size: 729 33 2274
Fragments arranged by size:
2274 729 33
BamHI G'GATC_C
Cuts at: 0 933 1548 2831 3036
Size: 933 615 1283 205
Fragments arranged by size:
1283 933 615 205
BanI G'GyrC_C
Cuts at: 0 406 1321 1696 2754 2943 3036
Size: 406 915 375 1058 189 93
Fragments arranged by size:
1058 915 406 375 189 93
BanII G_rGCy'C
Cuts at: 0 70 281 562 659 841 1075 1202 1752
Size: 70 211 281 97 182 234 127 550
Cuts at: 1752 1845 2019 2401 3036
Size: 93 174 382 635
Fragments arranged by size:
635 550 382 281 234 211 182 174
127 97 93 70
BbsI GAAGACnn'nnnn_
Cuts at: 0 1174 3036
Size: 1174 1862
BbvCI CC'TCA_GC
Cuts at: 0 1758 2065 3036
Size: 1758 307 971
Fragments arranged by size:
1758 971 307
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 199 233 1673 1707 2989 3023 3036
Size: 199 34 1440 34 1282 34 13
Fragments arranged by size:
1440 1282 199 34 34 34 13
BfrBI ATG'CAT
Cuts at: 0 2214 3036
Size: 2214 822
BglI GCCn_nnn'nGGC
Cuts at: 0 122 139 2591 3036
Size: 122 17 2452 445
Fragments arranged by size:
2452 445 122 17
BlpI GC'TnA_GC
Cuts at: 0 287 2847 2980 3036
Size: 287 2560 133 56
Fragments arranged by size:
2560 287 133 56
Bme1580I G_kGCm'C
Cuts at: 0 320 409 888 1227 1699 1845 2401 2520
Size: 320 89 479 339 472 146 556 119
Cuts at: 2520 3036
Size: 516
Fragments arranged by size:
556 516 479 472 339 320 146 119
89
BmrI ACTGGGnnnn_n'
Cuts at: 0 216 1202 1375 3036
Size: 216 986 173 1661
Fragments arranged by size:
1661 986 216 173
BmtI G_CTAG'C
Cuts at: 0 2477 3036
Size: 2477 559
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 406 727 799 1113 1699 2409 2617 2803
Size: 406 321 72 314 586 710 208 186
Cuts at: 2803 3036
Size: 233
Fragments arranged by size:
710 586 406 321 314 233 208 186
72
Bpu10I CC'TnA_GC
Cuts at: 0 1051 1758 2065 2351 3036
Size: 1051 707 307 286 685
Fragments arranged by size:
1051 707 685 307 286
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 257 1089 2113 3036
Size: 257 832 1024 923
Fragments arranged by size:
1024 923 832 257
BsaI GGTCTCn'nnnn_
Cuts at: 0 765 1876 3036
Size: 765 1111 1160
Fragments arranged by size:
1160 1111 765
BsaAI yAC'GTr
Cuts at: 0 687 2181 3036
Size: 687 1494 855
Fragments arranged by size:
1494 855 687
BsaHI Gr'CG_yC
Cuts at: 0 1293 3036
Size: 1293 1743
BsaWI w'CCGG_w
Cuts at: 0 1185 2505 3036
Size: 1185 1320 531
Fragments arranged by size:
1320 1185 531
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 86 116 608 638 685 715 1570 1600
Size: 86 30 492 30 47 30 855 30
Cuts at: 1600 3036
Size: 1436
Fragments arranged by size:
1436 855 492 86 47 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 49 220 403 2164 3036
Size: 49 171 183 1761 872
Fragments arranged by size:
1761 872 183 171 49
BseYI C'CCAG_C
Cuts at: 0 63 923 1089 2135 2550 2700 2761 2891
Size: 63 860 166 1046 415 150 61 130
Cuts at: 2891 3036
Size: 145
Fragments arranged by size:
1046 860 415 166 150 145 130 63
61
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 523 874 1079 1115 2228 2824 3007 3036
Size: 523 351 205 36 1113 596 183 29
Fragments arranged by size:
1113 596 523 351 205 183 36 29
BsiEI CG_ry'CG
Cuts at: 0 2867 3036
Size: 2867 169
BsiHKAI G_wGCw'C
Cuts at: 0 281 320 659 841 888 1202 1565 1829
Size: 281 39 339 182 47 314 363 264
Cuts at: 1829 3036
Size: 1207
Fragments arranged by size:
1207 363 339 314 281 264 182 47
39
BsmI GAATG_Cn'
Cuts at: 0 569 3036
Size: 569 2467
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1284 3036
Size: 1284 1752
Bsp1286I G_dGCh'C
Cuts at: 0 70 281 320 409 562 659 841 888
Size: 70 211 39 89 153 97 182 47
Cuts at: 888 1075 1202 1227 1565 1699 1752 1829 1845
Size: 187 127 25 338 134 53 77 16
Cuts at: 1845 2019 2401 2520 3036
Size: 174 382 119 516
Fragments arranged by size:
516 382 338 211 187 182 174 153
134 127 119 97 89 77 70 53
47 39 25 16
BspEI T'CCGG_A
Cuts at: 0 1185 3036
Size: 1185 1851
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 81 208 350 1051 2906 2955 3036
Size: 81 127 142 701 1855 49 81
Fragments arranged by size:
1855 701 142 127 81 81 49
BsrBI CCG'CTC
Cuts at: 0 1533 3036
Size: 1533 1503
BsrDI GCAATG_nn'
Cuts at: 0 2555 3036
Size: 2555 481
BsrFI r'CCGG_y
Cuts at: 0 914 2505 3036
Size: 914 1591 531
Fragments arranged by size:
1591 914 531
BssSI C'ACGA_G
Cuts at: 0 460 3036
Size: 460 2576
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1102 1495 1833 2220 3036
Size: 1102 393 338 387 816
Fragments arranged by size:
1102 816 393 387 338
BstBI TT'CG_AA
Cuts at: 0 19 3036
Size: 19 3017
BstEII G'GTnAC_C
Cuts at: 0 2873 3036
Size: 2873 163
BstXI CCAn_nnnn'nTGG
Cuts at: 0 105 150 1462 1994 2215 3036
Size: 105 45 1312 532 221 821
Fragments arranged by size:
1312 821 532 221 105 45
BstYI r'GATC_y
Cuts at: 0 933 1548 2831 3036
Size: 933 615 1283 205
Fragments arranged by size:
1283 933 615 205
Bsu36I CC'TnA_GG
Cuts at: 0 1214 2905 3036
Size: 1214 1691 131
Fragments arranged by size:
1691 1214 131
BtgI C'CryG_G
Cuts at: 0 909 2623 3036
Size: 909 1714 413
Fragments arranged by size:
1714 909 413
BtsI GCAGTG_nn'
Cuts at: 0 861 886 1062 1265 1672 1713 1843 3005
Size: 861 25 176 203 407 41 130 1162
Cuts at: 3005 3036
Size: 31
Fragments arranged by size:
1162 861 407 203 176 130 41 31
25
DraIII CAC_nnn'GTG
Cuts at: 0 413 3036
Size: 413 2623
DrdI GACnn_nn'nnGTC
Cuts at: 0 2784 3036
Size: 2784 252
EaeI y'GGCC_r
Cuts at: 0 140 912 916 1082 2022 2864 3036
Size: 140 772 4 166 940 842 172
Fragments arranged by size:
940 842 772 172 166 140 4
EagI C'GGCC_G
Cuts at: 0 2864 3036
Size: 2864 172
EarI CTCTTCn'nnn_
Cuts at: 0 995 1007 1450 1870 3036
Size: 995 12 443 420 1166
Fragments arranged by size:
1166 995 443 420 12
EcoICRI GAG'CTC
Cuts at: 0 279 657 839 1200 3036
Size: 279 378 182 361 1836
Fragments arranged by size:
1836 378 361 279 182
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 406 418 727 782 799 1006 1113 1138
Size: 406 12 309 55 17 207 107 25
Cuts at: 1138 1431 1687 1699 1975 2281 2409 2428 2617
Size: 293 256 12 276 306 128 19 189
Cuts at: 2617 2746 2770 2788 2803 3036
Size: 129 24 18 15 233
Fragments arranged by size:
406 309 306 293 276 256 233 207
189 129 128 107 55 25 24 19
18 17 15 12 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 871 3036
Size: 871 2165
EcoO109I rG'GnC_Cy
Cuts at: 0 102 123 339 456 507 701 972 1029
Size: 102 21 216 117 51 194 271 57
Cuts at: 1029 1451 1503 1971 2119 2398 2777 3036
Size: 422 52 468 148 279 379 259
Fragments arranged by size:
468 422 379 279 271 259 216 194
148 117 102 57 52 51 21
FseI GG_CCGG'CC
Cuts at: 0 918 3036
Size: 918 2118
HaeII r_GCGC'y
Cuts at: 0 1319 1422 1638 3036
Size: 1319 103 216 1398
Fragments arranged by size:
1398 1319 216 103
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 86 118 661 685 693 717 1968 2000
Size: 86 32 543 24 8 24 1251 32
Cuts at: 2000 2703 2735 3036
Size: 703 32 301
Fragments arranged by size:
1251 703 543 301 86 32 32 32
24 24 8
HincII GTy'rAC
Cuts at: 0 1401 2511 3036
Size: 1401 1110 525
Fragments arranged by size:
1401 1110 525
KpnI G_GTAC'C
Cuts at: 0 2758 3036
Size: 2758 278
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 233 329 1893 3036
Size: 233 96 1564 1143
Fragments arranged by size:
1564 1143 233 96
MscI TGG'CCA
Cuts at: 0 142 1084 2024 3036
Size: 142 942 940 1012
Fragments arranged by size:
1012 942 940 142
MslI CAynn'nnrTG
Cuts at: 0 323 422 908 1568 3036
Size: 323 99 486 660 1468
Fragments arranged by size:
1468 660 486 323 99
MspA1I CmG'CkG
Cuts at: 0 54 396 535 1382 1495 1762 2061 2191
Size: 54 342 139 847 113 267 299 130
Cuts at: 2191 2575 2761 2931 2961 3036
Size: 384 186 170 30 75
Fragments arranged by size:
847 384 342 299 267 186 170 139
130 113 75 54 30
NaeI GCC'GGC
Cuts at: 0 916 3036
Size: 916 2120
NcoI C'CATG_G
Cuts at: 0 909 3036
Size: 909 2127
NgoMIV G'CCGG_C
Cuts at: 0 914 3036
Size: 914 2122
NheI G'CTAG_C
Cuts at: 0 2473 3036
Size: 2473 563
NsiI A_TGCA'T
Cuts at: 0 2216 3036
Size: 2216 820
NspI r_CATG'y
Cuts at: 0 1732 3036
Size: 1732 1304
PasI CC'CwG_GG
Cuts at: 0 555 929 3036
Size: 555 374 2107
Fragments arranged by size:
2107 555 374
PciI A'CATG_T
Cuts at: 0 1728 3036
Size: 1728 1308
PflMI CCAn_nnn'nTGG
Cuts at: 0 203 363 828 1966 2142 2292 3036
Size: 203 160 465 1138 176 150 744
Fragments arranged by size:
1138 744 465 203 176 160 150
PfoI T'CCnGG_A
Cuts at: 0 1129 3036
Size: 1129 1907
PmlI CAC'GTG
Cuts at: 0 687 3036
Size: 687 2349
PpuMI rG'GwC_Cy
Cuts at: 0 102 339 507 701 972 1451 1971 2119
Size: 102 237 168 194 271 479 520 148
Cuts at: 2119 2777 3036
Size: 658 259
Fragments arranged by size:
658 520 479 271 259 237 194 168
148 102
PshAI GACnn'nnGTC
Cuts at: 0 792 3036
Size: 792 2244
PspOMI G'GGCC_C
Cuts at: 0 1841 2397 3036
Size: 1841 556 639
Fragments arranged by size:
1841 639 556
PstI C_TGCA'G
Cuts at: 0 122 1491 1767 1838 1881 3036
Size: 122 1369 276 71 43 1155
Fragments arranged by size:
1369 1155 276 122 71 43
PvuII CAG'CTG
Cuts at: 0 54 396 535 1495 1762 2061 2191 2575
Size: 54 342 139 960 267 299 130 384
Cuts at: 2575 2761 2931 3036
Size: 186 170 105
Fragments arranged by size:
960 384 342 299 267 186 170 139
130 105 54
SacI G_AGCT'C
Cuts at: 0 281 659 841 1202 3036
Size: 281 378 182 361 1834
Fragments arranged by size:
1834 378 361 281 182
SanDI GG'GwC_CC
Cuts at: 0 701 3036
Size: 701 2335
SapI GCTCTTCn'nnn_
Cuts at: 0 995 1007 3036
Size: 995 12 2029
Fragments arranged by size:
2029 995 12
SbfI CC_TGCA'GG
Cuts at: 0 122 3036
Size: 122 2914
SfcI C'TryA_G
Cuts at: 0 118 953 1487 1712 1743 1763 1834 1877
Size: 118 835 534 225 31 20 71 43
Cuts at: 1877 2567 3036
Size: 690 469
Fragments arranged by size:
835 690 534 469 225 118 71 43
31 20
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 122 139 2591 3036
Size: 122 17 2452 445
Fragments arranged by size:
2452 445 122 17
SmaI CCC'GGG
Cuts at: 0 454 970 1501 3036
Size: 454 516 531 1535
Fragments arranged by size:
1535 531 516 454
SmlI C'TyrA_G
Cuts at: 0 272 1068 2092 3036
Size: 272 796 1024 944
Fragments arranged by size:
1024 944 796 272
SnaBI TAC'GTA
Cuts at: 0 2181 3036
Size: 2181 855
StuI AGG'CCT
Cuts at: 0 116 268 877 1741 2585 3036
Size: 116 152 609 864 844 451
Fragments arranged by size:
864 844 609 451 152 116
StyI C'CwwG_G
Cuts at: 0 909 1472 2141 3036
Size: 909 563 669 895
Fragments arranged by size:
909 895 669 563
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 151 1081 1672 3036
Size: 151 930 591 1364
Fragments arranged by size:
1364 930 591 151
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 608 640 3036
Size: 608 32 2396
Fragments arranged by size:
2396 608 32
XbaI T'CTAG_A
Cuts at: 0 2316 3036
Size: 2316 720
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 562 907 926 1479 3036
Size: 562 345 19 553 1557
Fragments arranged by size:
1557 562 553 345 19
XmaI C'CCGG_G
Cuts at: 0 452 968 1499 3036
Size: 452 516 531 1537
Fragments arranged by size:
1537 531 516 452
XmnI GAAnn'nnTTC
Cuts at: 0 2921 3036
Size: 2921 115
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AflIII AgeI AleI
AlwNI ApaI ApaLI AvaI BaeI BamHI BanI BanII
BbsI BbvCI BcgI BfrBI BglI BlpI Bme1580I BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtsI DraIII
DrdI EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I
FseI HaeII Hin4I HincII KpnI MmeI MscI MslI
MspA1I NaeI NcoI NgoMIV NheI NsiI NspI PasI
PciI PflMI PfoI PmlI PpuMI PshAI PspOMI PstI
PvuII SacI SanDI SapI SbfI SfcI SfiI SmaI
SmlI SnaBI StuI StyI TaqII TstI XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII AclI AflII AhdI AlfI AloI ApoI AscI
AseI AsiSI AvrII BbeI BciVI BclI BglII BmgBI
BplI BsaBI BsiWI BspHI BsrGI BssHII BstZ17I BtgZI
ClaI CspCI DraI EciI EcoRI EcoRV FalI FspI
FspAI HindIII HpaI KasI MfeI MluI NarI NdeI
NotI NruI PacI PmeI PpiI PsiI PspXI PsrI
PvuI RsrII SacII SalI ScaI SexAI SfoI SgfI
SgrAI SpeI SphI SrfI SspI SwaI TatI Tth111I
XhoI ZraI