(Linear) (Six-base) MAPSORT of: KIEE3654.seq  Check: 4040  from: 1  to: 732



KIEE3654.orf

 With 182 enzymes: SgfI * 

                        December 27, 2011 16:37  ..

AccI GT'mk_AC

Cuts at:      0     677     732 
   Size:        677      55
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     187     732 
   Size:        187     545
 
AflIII A'CryG_T

Cuts at:      0     536     732 
   Size:        536     196
 
AvaI C'yCGr_G

Cuts at:      0     123     732 
   Size:        123     609
 
BamHI G'GATC_C

Cuts at:      0     707     732 
   Size:        707      25
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     201     732 
   Size:        201     531
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     211     245     732 
   Size:        211      34     487

  Fragments arranged by size: 

                 487     211      34
 
BlpI GC'TnA_GC

Cuts at:      0     393     732 
   Size:        393     339
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     634     732 
   Size:        634      98
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     400     732 
   Size:        400     332
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     605     732 
   Size:        605     127
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      64     511     732 
   Size:         64     447     221

  Fragments arranged by size: 

                 447     221      64
 
BseYI C'CCAG_C

Cuts at:      0     342     425     547     732 
   Size:        342      83     122     185

  Fragments arranged by size: 

                 342     185     122      83
 
BstYI r'GATC_y

Cuts at:      0     707     732 
   Size:        707      25
 
BtsI GCAGTG_nn'

Cuts at:      0     183     732 
   Size:        183     549
 
DraIII CAC_nnn'GTG

Cuts at:      0     486     732 
   Size:        486     246
 
EaeI y'GGCC_r

Cuts at:      0     519     732 
   Size:        519     213
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      89     732 
   Size:         89     643
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     187     400     732 
   Size:        187     213     332

  Fragments arranged by size: 

                 332     213     187
 
EcoO109I rG'GnC_Cy

Cuts at:      0     147     168     732 
   Size:        147      21     564

  Fragments arranged by size: 

                 564     147      21
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      76     108     732 
   Size:         76      32     624

  Fragments arranged by size: 

                 624      76      32
 
MscI TGG'CCA

Cuts at:      0     521     732 
   Size:        521     211
 
MspA1I CmG'CkG

Cuts at:      0     178     410     600     732 
   Size:        178     232     190     132

  Fragments arranged by size: 

                 232     190     178     132
 
NspI r_CATG'y

Cuts at:      0     511     540     732 
   Size:        511      29     192

  Fragments arranged by size: 

                 511     192      29
 
PciI A'CATG_T

Cuts at:      0     536     732 
   Size:        536     196
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     420     732 
   Size:        420     312
 
PpuMI rG'GwC_Cy

Cuts at:      0     147     168     732 
   Size:        147      21     564

  Fragments arranged by size: 

                 564     147      21
 
PstI C_TGCA'G

Cuts at:      0      39     694     732 
   Size:         39     655      38

  Fragments arranged by size: 

                 655      39      38
 
PvuII CAG'CTG

Cuts at:      0     178     410     732 
   Size:        178     232     322

  Fragments arranged by size: 

                 322     232     178
 
SexAI A'CCwGG_T

Cuts at:      0     482     732 
   Size:        482     250
 
SfcI C'TryA_G

Cuts at:      0      35     690     732 
   Size:         35     655      42

  Fragments arranged by size: 

                 655      42      35
 
SphI G_CATG'C

Cuts at:      0     511     732 
   Size:        511     221
 
StuI AGG'CCT

Cuts at:      0     626     732 
   Size:        626     106
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     544     732 
   Size:        544     188
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     152     184     472     504     732 
   Size:        152      32     288      32     228

  Fragments arranged by size: 

                 288     228     152      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     136     586     732 
   Size:        136     450     146

  Fragments arranged by size: 

                 450     146     136
 

 Enzymes that do cut:

     AccI     AcuI   AflIII     AvaI    BamHI     BbsI     BcgI     BlpI
     BplI     BpmI     BsaI    BseRI    BseYI    BstYI     BtsI   DraIII
     EaeI     EciI  Eco57MI EcoO109I    Hin4I     MscI   MspA1I     NspI
     PciI    PflMI    PpuMI     PstI    PvuII    SexAI     SfcI     SphI
     StuI    TaqII     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI     BanI    BanII     BbeI    BbvCI
    BciVI     BclI    BfrBI     BglI    BglII Bme1580I    BmgBI     BmrI
     BmtI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI
     ClaI    CspCI     DraI     DrdI     EagI     EarI  EcoICRI    EcoNI
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MslI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PasI     PfoI     PmeI     PmlI     PpiI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SrfI     SspI     StyI
     SwaI     TatI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI