Restriction map for
pFN21AE3650
(Linear) (Six-base) MAPSORT of: KIEE3650.seq Check: 1532 from: 1 to: 2010
KIEE3650.orf
With 182 enzymes: SgfI *
March 22, 2012 16:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1509 2010
Size: 1509 501
AccI GT'mk_AC
Cuts at: 0 1463 2010
Size: 1463 547
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 484 1922 2010
Size: 484 1438 88
Fragments arranged by size:
1438 484 88
AleI CACnn'nnGTG
Cuts at: 0 1552 2010
Size: 1552 458
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 16 48 2010
Size: 16 32 1962
Fragments arranged by size:
1962 32 16
AlwNI CAG_nnn'CTG
Cuts at: 0 1431 2010
Size: 1431 579
ApaI G_GGCC'C
Cuts at: 0 655 2010
Size: 655 1355
AvaI C'yCGr_G
Cuts at: 0 19 316 1201 1306 2010
Size: 19 297 885 105 704
Fragments arranged by size:
885 704 297 105 19
BanI G'GyrC_C
Cuts at: 0 91 639 681 1019 1484 2010
Size: 91 548 42 338 465 526
Fragments arranged by size:
548 526 465 338 91 42
BanII G_rGCy'C
Cuts at: 0 298 510 655 1121 1139 1202 1268 1406
Size: 298 212 145 466 18 63 66 138
Cuts at: 1406 2010
Size: 604
Fragments arranged by size:
604 466 298 212 145 138 66 63
18
BbeI G_GCGC'C
Cuts at: 0 95 643 685 1023 2010
Size: 95 548 42 338 987
Fragments arranged by size:
987 548 338 95 42
BbsI GAAGACnn'nnnn_
Cuts at: 0 478 598 1910 2010
Size: 478 120 1312 100
Fragments arranged by size:
1312 478 120 100
BglI GCCn_nnn'nGGC
Cuts at: 0 319 514 864 885 2010
Size: 319 195 350 21 1125
Fragments arranged by size:
1125 350 319 195 21
BlpI GC'TnA_GC
Cuts at: 0 1085 2010
Size: 1085 925
Bme1580I G_kGCm'C
Cuts at: 0 655 1977 2010
Size: 655 1322 33
Fragments arranged by size:
1322 655 33
BmgBI CAC'GTC
Cuts at: 0 1476 1554 2010
Size: 1476 78 456
Fragments arranged by size:
1476 456 78
BmrI ACTGGGnnnn_n'
Cuts at: 0 1795 2010
Size: 1795 215
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 444 582 671 1386 2010
Size: 444 138 89 715 624
Fragments arranged by size:
715 624 444 138 89
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 504 885 1000 1234 1522 2010
Size: 504 381 115 234 288 488
Fragments arranged by size:
504 488 381 288 234 115
Bpu10I CC'TnA_GC
Cuts at: 0 734 1120 2010
Size: 734 386 890
Fragments arranged by size:
890 734 386
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1239 1621 2010
Size: 1239 382 389
Fragments arranged by size:
1239 389 382
BsaI GGTCTCn'nnnn_
Cuts at: 0 501 2010
Size: 501 1509
BsaHI Gr'CG_yC
Cuts at: 0 92 475 640 682 760 1020 2010
Size: 92 383 165 42 78 260 990
Fragments arranged by size:
990 383 260 165 92 78 42
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 688 718 2010
Size: 688 30 1292
Fragments arranged by size:
1292 688 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 288 574 756 759 762 771 774 777
Size: 288 286 182 3 3 9 3 3
Cuts at: 777 1021 1024 1057 1176 1324 1429 1516 1729
Size: 244 3 33 119 148 105 87 213
Cuts at: 1729 1747 1762 1873 1887 1891 2010
Size: 18 15 111 14 4 119
Fragments arranged by size:
288 286 244 213 182 148 119 119
111 105 87 33 18 15 14 9
4 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 1506 1690 1780 2010
Size: 1506 184 90 230
Fragments arranged by size:
1506 230 184 90
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1417 1693 1894 2010
Size: 1417 276 201 116
Fragments arranged by size:
1417 276 201 116
BsiEI CG_ry'CG
Cuts at: 0 757 1074 1956 2010
Size: 757 317 882 54
Fragments arranged by size:
882 757 317 54
BsiHKAI G_wGCw'C
Cuts at: 0 298 1121 1856 2010
Size: 298 823 735 154
Fragments arranged by size:
823 735 298 154
BsiWI C'GTAC_G
Cuts at: 0 1917 2010
Size: 1917 93
BsmI GAATG_Cn'
Cuts at: 0 1497 2010
Size: 1497 513
Bsp1286I G_dGCh'C
Cuts at: 0 298 510 655 1121 1139 1202 1268 1406
Size: 298 212 145 466 18 63 66 138
Cuts at: 1406 1856 1977 2010
Size: 450 121 33
Fragments arranged by size:
466 450 298 212 145 138 121 66
63 33 18
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1509 1916 2010
Size: 1509 407 94
Fragments arranged by size:
1509 407 94
BsrBI CCG'CTC
Cuts at: 0 1053 1065 1089 1344 2010
Size: 1053 12 24 255 666
Fragments arranged by size:
1053 666 255 24 12
BsrFI r'CCGG_y
Cuts at: 0 738 1955 2010
Size: 738 1217 55
Fragments arranged by size:
1217 738 55
BssSI C'ACGA_G
Cuts at: 0 1855 2010
Size: 1855 155
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1431 2010
Size: 1431 579
BstEII G'GTnAC_C
Cuts at: 0 390 1521 2010
Size: 390 1131 489
Fragments arranged by size:
1131 489 390
BstXI CCAn_nnnn'nTGG
Cuts at: 0 528 2010
Size: 528 1482
BstYI r'GATC_y
Cuts at: 0 248 1532 2010
Size: 248 1284 478
Fragments arranged by size:
1284 478 248
BtgI C'CryG_G
Cuts at: 0 643 746 1074 1409 2010
Size: 643 103 328 335 601
Fragments arranged by size:
643 601 335 328 103
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 66 2010
Size: 66 1944
BtsI GCAGTG_nn'
Cuts at: 0 345 912 2010
Size: 345 567 1098
Fragments arranged by size:
1098 567 345
DrdI GACnn_nn'nnGTC
Cuts at: 0 370 2010
Size: 370 1640
EaeI y'GGCC_r
Cuts at: 0 377 743 1281 1559 1724 1739 2010
Size: 377 366 538 278 165 15 271
Fragments arranged by size:
538 377 366 278 271 165 15
EarI CTCTTCn'nnn_
Cuts at: 0 460 551 780 1288 1474 2010
Size: 460 91 229 508 186 536
Fragments arranged by size:
536 508 460 229 186 91
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 275 882 1783 2010
Size: 275 607 901 227
Fragments arranged by size:
901 607 275 227
EcoICRI GAG'CTC
Cuts at: 0 296 1119 2010
Size: 296 823 891
Fragments arranged by size:
891 823 296
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 484 504 885 1000 1234 1522 1922 2010
Size: 484 20 381 115 234 288 400 88
Fragments arranged by size:
484 400 381 288 234 115 88 20
EcoNI CCTnn'n_nnAGG
Cuts at: 0 532 1999 2010
Size: 532 1467 11
Fragments arranged by size:
1467 532 11
EcoO109I rG'GnC_Cy
Cuts at: 0 160 168 312 651 652 1174 1647 1752
Size: 160 8 144 339 1 522 473 105
Cuts at: 1752 1927 1992 2010
Size: 175 65 18
Fragments arranged by size:
522 473 339 175 160 144 105 65
18 8 1
HaeII r_GCGC'y
Cuts at: 0 95 281 643 685 1023 2010
Size: 95 186 362 42 338 987
Fragments arranged by size:
987 362 338 186 95 42
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 418 450 688 720 1804 1836 2010
Size: 418 32 238 32 1084 32 174
Fragments arranged by size:
1084 418 238 174 32 32 32
HincII GTy'rAC
Cuts at: 0 1464 2010
Size: 1464 546
KasI G'GCGC_C
Cuts at: 0 91 639 681 1019 2010
Size: 91 548 42 338 991
Fragments arranged by size:
991 548 338 91 42
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 326 566 719 725 815 2010
Size: 326 240 153 6 90 1195
Fragments arranged by size:
1195 326 240 153 90 6
MscI TGG'CCA
Cuts at: 0 745 1283 1561 2010
Size: 745 538 278 449
Fragments arranged by size:
745 538 449 278
MslI CAynn'nnrTG
Cuts at: 0 1552 2010
Size: 1552 458
MspA1I CmG'CkG
Cuts at: 0 388 403 631 838 1076 1097 1109 2010
Size: 388 15 228 207 238 21 12 901
Fragments arranged by size:
901 388 238 228 207 21 15 12
NarI GG'CG_CC
Cuts at: 0 92 640 682 1020 2010
Size: 92 548 42 338 990
Fragments arranged by size:
990 548 338 92 42
NcoI C'CATG_G
Cuts at: 0 746 2010
Size: 746 1264
PflMI CCAn_nnn'nTGG
Cuts at: 0 1807 1891 2010
Size: 1807 84 119
Fragments arranged by size:
1807 119 84
PpuMI rG'GwC_Cy
Cuts at: 0 160 168 1174 1647 1927 2010
Size: 160 8 1006 473 280 83
Fragments arranged by size:
1006 473 280 160 83 8
PspOMI G'GGCC_C
Cuts at: 0 651 2010
Size: 651 1359
PstI C_TGCA'G
Cuts at: 0 1427 1520 2010
Size: 1427 93 490
Fragments arranged by size:
1427 490 93
PvuII CAG'CTG
Cuts at: 0 403 2010
Size: 403 1607
RsrII CG'GwC_CG
Cuts at: 0 633 1358 2010
Size: 633 725 652
Fragments arranged by size:
725 652 633
SacI G_AGCT'C
Cuts at: 0 298 1121 2010
Size: 298 823 889
Fragments arranged by size:
889 823 298
SacII CC_GC'GG
Cuts at: 0 1077 2010
Size: 1077 933
SalI G'TCGA_C
Cuts at: 0 1462 2010
Size: 1462 548
SanDI GG'GwC_CC
Cuts at: 0 160 1174 1927 2010
Size: 160 1014 753 83
Fragments arranged by size:
1014 753 160 83
SbfI CC_TGCA'GG
Cuts at: 0 1520 2010
Size: 1520 490
SfcI C'TryA_G
Cuts at: 0 273 1423 1516 2010
Size: 273 1150 93 494
Fragments arranged by size:
1150 494 273 93
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 319 2010
Size: 319 1691
SfoI GGC'GCC
Cuts at: 0 93 641 683 1021 2010
Size: 93 548 42 338 989
Fragments arranged by size:
989 548 338 93 42
SmaI CCC'GGG
Cuts at: 0 21 2010
Size: 21 1989
SmlI C'TyrA_G
Cuts at: 0 1254 1636 2010
Size: 1254 382 374
Fragments arranged by size:
1254 382 374
StuI AGG'CCT
Cuts at: 0 517 1944 2010
Size: 517 1427 66
Fragments arranged by size:
1427 517 66
StyI C'CwwG_G
Cuts at: 0 164 707 746 1885 2010
Size: 164 543 39 1139 125
Fragments arranged by size:
1139 543 164 125 39
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 123 483 2010
Size: 123 360 1527
Fragments arranged by size:
1527 360 123
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 414 2010
Size: 414 1596
XmaI C'CCGG_G
Cuts at: 0 19 2010
Size: 19 1991
XmnI GAAnn'nnTTC
Cuts at: 0 226 2010
Size: 226 1784
Enzymes that do cut:
AarI AccI AcuI AleI AloI AlwNI ApaI AvaI
BanI BanII BbeI BbsI BglI BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I
BspMI BsrBI BsrFI BssSI BstAPI BstEII BstXI BstYI
BtgI BtgZI BtsI DrdI EaeI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I HaeII Hin4I HincII KasI MmeI
MscI MslI MspA1I NarI NcoI PflMI PpuMI PspOMI
PstI PvuII RsrII SacI SacII SalI SanDI SbfI
SfcI SfiI SfoI SmaI SmlI StuI StyI TaqII
XcmI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AlfI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbvCI BcgI BciVI BclI BfrBI BglII BmtI
BsaAI BsaBI BsaWI BsmBI BspEI BspHI BsrDI BsrGI
BssHII BstBI BstZ17I Bsu36I ClaI CspCI DraI DraIII
EagI EcoRI EcoRV FalI FseI FspI FspAI HindIII
HpaI KpnI MfeI MluI NaeI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PasI PciI PfoI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI
SapI ScaI SexAI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI SwaI TatI TstI Tth111I XbaI XhoI
ZraI