Restriction map for
pFN21AE3649
(Linear) (Six-base) MAPSORT of: KIEE3649.seq Check: 9889 from: 1 to: 1311
KIEE3649.orf
With 182 enzymes: SgfI *
December 27, 2011 16:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1096 1311
Size: 1096 215
Acc65I G'GTAC_C
Cuts at: 0 723 1311
Size: 723 588
AclI AA'CG_TT
Cuts at: 0 795 1311
Size: 795 516
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 159 517 571 1311
Size: 159 358 54 740
Fragments arranged by size:
740 358 159 54
AflII C'TTAA_G
Cuts at: 0 1276 1311
Size: 1276 35
AlwNI CAG_nnn'CTG
Cuts at: 0 387 1002 1300 1311
Size: 387 615 298 11
Fragments arranged by size:
615 387 298 11
AvaI C'yCGr_G
Cuts at: 0 82 1311
Size: 82 1229
BamHI G'GATC_C
Cuts at: 0 317 1311
Size: 317 994
BanI G'GyrC_C
Cuts at: 0 490 723 1311
Size: 490 233 588
Fragments arranged by size:
588 490 233
BanII G_rGCy'C
Cuts at: 0 406 920 1311
Size: 406 514 391
Fragments arranged by size:
514 406 391
BbsI GAAGACnn'nnnn_
Cuts at: 0 165 1311
Size: 165 1146
BciVI GTATCCnnnnn_n'
Cuts at: 0 449 1311
Size: 449 862
BfrBI ATG'CAT
Cuts at: 0 475 579 1311
Size: 475 104 732
Fragments arranged by size:
732 475 104
BglI GCCn_nnn'nGGC
Cuts at: 0 1103 1311
Size: 1103 208
Bme1580I G_kGCm'C
Cuts at: 0 673 1036 1169 1311
Size: 673 363 133 142
Fragments arranged by size:
673 363 142 133
BmgBI CAC'GTC
Cuts at: 0 131 1311
Size: 131 1180
BmrI ACTGGGnnnn_n'
Cuts at: 0 711 1189 1311
Size: 711 478 122
Fragments arranged by size:
711 478 122
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 409 638 1311
Size: 409 229 673
Fragments arranged by size:
673 409 229
Bpu10I CC'TnA_GC
Cuts at: 0 967 1099 1311
Size: 967 132 212
Fragments arranged by size:
967 212 132
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 769 1311
Size: 769 542
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 578 608 1311
Size: 578 30 703
Fragments arranged by size:
703 578 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 437 511 1285 1311
Size: 437 74 774 26
Fragments arranged by size:
774 437 74 26
BseYI C'CCAG_C
Cuts at: 0 1118 1311
Size: 1118 193
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 307 928 1311
Size: 307 621 383
Fragments arranged by size:
621 383 307
BsiHKAI G_wGCw'C
Cuts at: 0 89 1311
Size: 89 1222
Bsp1286I G_dGCh'C
Cuts at: 0 89 406 673 920 1036 1169 1311
Size: 89 317 267 247 116 133 142
Fragments arranged by size:
317 267 247 142 133 116 89
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1096 1311
Size: 1096 215
BsrBI CCG'CTC
Cuts at: 0 99 1103 1311
Size: 99 1004 208
Fragments arranged by size:
1004 208 99
BsrFI r'CCGG_y
Cuts at: 0 836 1311
Size: 836 475
BsrGI T'GTAC_A
Cuts at: 0 68 1199 1311
Size: 68 1131 112
Fragments arranged by size:
1131 112 68
BstAPI GCAn_nnn'nTGC
Cuts at: 0 472 616 1002 1300 1311
Size: 472 144 386 298 11
Fragments arranged by size:
472 386 298 144 11
BstEII G'GTnAC_C
Cuts at: 0 955 1311
Size: 955 356
BstYI r'GATC_y
Cuts at: 0 161 317 1311
Size: 161 156 994
Fragments arranged by size:
994 161 156
BtgI C'CryG_G
Cuts at: 0 301 741 1311
Size: 301 440 570
Fragments arranged by size:
570 440 301
BtsI GCAGTG_nn'
Cuts at: 0 671 940 1311
Size: 671 269 371
Fragments arranged by size:
671 371 269
DraIII CAC_nnn'GTG
Cuts at: 0 1197 1311
Size: 1197 114
EaeI y'GGCC_r
Cuts at: 0 767 1311
Size: 767 544
EarI CTCTTCn'nnn_
Cuts at: 0 805 1311
Size: 805 506
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 159 409 517 571 638 1311
Size: 159 250 108 54 67 673
Fragments arranged by size:
673 250 159 108 67 54
EcoNI CCTnn'n_nnAGG
Cuts at: 0 664 1311
Size: 664 647
EcoO109I rG'GnC_Cy
Cuts at: 0 427 999 1311
Size: 427 572 312
Fragments arranged by size:
572 427 312
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 975 1007 1273 1305 1311
Size: 975 32 266 32 6
Fragments arranged by size:
975 266 32 32 6
HindIII A'AGCT_T
Cuts at: 0 526 1311
Size: 526 785
KpnI G_GTAC'C
Cuts at: 0 727 1311
Size: 727 584
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 564 1311
Size: 564 747
MslI CAynn'nnrTG
Cuts at: 0 1018 1311
Size: 1018 293
MspA1I CmG'CkG
Cuts at: 0 66 872 1133 1311
Size: 66 806 261 178
Fragments arranged by size:
806 261 178 66
NaeI GCC'GGC
Cuts at: 0 838 1311
Size: 838 473
NcoI C'CATG_G
Cuts at: 0 301 741 1311
Size: 301 440 570
Fragments arranged by size:
570 440 301
NdeI CA'TA_TG
Cuts at: 0 459 1311
Size: 459 852
NgoMIV G'CCGG_C
Cuts at: 0 836 1311
Size: 836 475
NsiI A_TGCA'T
Cuts at: 0 477 581 1311
Size: 477 104 730
Fragments arranged by size:
730 477 104
NspI r_CATG'y
Cuts at: 0 583 1311
Size: 583 728
PflMI CCAn_nnn'nTGG
Cuts at: 0 363 412 1311
Size: 363 49 899
Fragments arranged by size:
899 363 49
PfoI T'CCnGG_A
Cuts at: 0 386 1311
Size: 386 925
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 780 812 1311
Size: 780 32 499
Fragments arranged by size:
780 499 32
PpuMI rG'GwC_Cy
Cuts at: 0 427 1311
Size: 427 884
PstI C_TGCA'G
Cuts at: 0 1007 1184 1311
Size: 1007 177 127
Fragments arranged by size:
1007 177 127
SapI GCTCTTCn'nnn_
Cuts at: 0 805 1311
Size: 805 506
SbfI CC_TGCA'GG
Cuts at: 0 1007 1311
Size: 1007 304
SfcI C'TryA_G
Cuts at: 0 368 1003 1180 1246 1311
Size: 368 635 177 66 65
Fragments arranged by size:
635 368 177 66 65
SmlI C'TyrA_G
Cuts at: 0 784 1276 1311
Size: 784 492 35
Fragments arranged by size:
784 492 35
StuI AGG'CCT
Cuts at: 0 254 502 1311
Size: 254 248 809
Fragments arranged by size:
809 254 248
StyI C'CwwG_G
Cuts at: 0 205 301 741 951 1311
Size: 205 96 440 210 360
Fragments arranged by size:
440 360 210 205 96
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 468 816 1150 1208 1311
Size: 468 348 334 58 103
Fragments arranged by size:
468 348 334 103 58
TatI w'GTAC_w
Cuts at: 0 68 542 1010 1199 1311
Size: 68 474 468 189 112
Fragments arranged by size:
474 468 189 112 68
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 578 610 1311
Size: 578 32 701
Fragments arranged by size:
701 578 32
Tth111I GACn'n_nGTC
Cuts at: 0 431 1311
Size: 431 880
XmnI GAAnn'nnTTC
Cuts at: 0 232 1311
Size: 232 1079
Enzymes that do cut:
AarI Acc65I AclI AcuI AflII AlwNI AvaI BamHI
BanI BanII BbsI BciVI BfrBI BglI Bme1580I BmgBI
BmrI BpmI Bpu10I BpuEI BsaXI BseRI BseYI BsgI
BsiHKAI Bsp1286I BspMI BsrBI BsrFI BsrGI BstAPI BstEII
BstYI BtgI BtsI DraIII EaeI EarI Eco57MI EcoNI
EcoO109I Hin4I HindIII KpnI MmeI MslI MspA1I NaeI
NcoI NdeI NgoMIV NsiI NspI PflMI PfoI PpiI
PpuMI PstI SapI SbfI SfcI SmlI StuI StyI
TaqII TatI TstI Tth111I XmnI
Enzymes that do not cut:
AatII AccI AfeI AflIII AgeI AhdI AleI AlfI
AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BbeI BbvCI BcgI BclI BglII BlpI BmtI
BplI BsaI BsaAI BsaBI BsaHI BsaWI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BsrDI BssHII BssSI BstBI
BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI
EagI EciI EcoICRI EcoRI EcoRV FalI FseI FspI
FspAI HaeII HincII HpaI KasI MfeI MluI MscI
NarI NheI NotI NruI PacI PasI PciI PmeI
PmlI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII
RsrII SacI SacII SalI SanDI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI SwaI XbaI XcmI XhoI XmaI ZraI