(Linear) (Six-base) MAPSORT of: KIEE3647.seq  Check: 4205  from: 1  to: 297



KIEE3647.orf

 With 182 enzymes: SgfI * 

                        December 27, 2011 16:37  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      44     183     266     297 
   Size:         44     139      83      31

  Fragments arranged by size: 

                 139      83      44      31
 
AlwNI CAG_nnn'CTG

Cuts at:      0     182     297 
   Size:        182     115
 
AvaI C'yCGr_G

Cuts at:      0       8      53     297 
   Size:          8      45     244

  Fragments arranged by size: 

                 244      45       8
 
BglI GCCn_nnn'nGGC

Cuts at:      0      34     172     297 
   Size:         34     138     125

  Fragments arranged by size: 

                 138     125      34
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      93     297 
   Size:         93     204
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     240     297 
   Size:        240      57
 
BseYI C'CCAG_C

Cuts at:      0     167     182     297 
   Size:        167      15     115

  Fragments arranged by size: 

                 167     115      15
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     169     297 
   Size:        169     128
 
BsiHKAI G_wGCw'C

Cuts at:      0     226     297 
   Size:        226      71
 
Bsp1286I G_dGCh'C

Cuts at:      0     226     297 
   Size:        226      71
 
BstEII G'GTnAC_C

Cuts at:      0     186     297 
   Size:        186     111
 
Bsu36I CC'TnA_GG

Cuts at:      0     266     297 
   Size:        266      31
 
BtgI C'CryG_G

Cuts at:      0     287     297 
   Size:        287      10
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     268     297 
   Size:        268      29
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      44     183     240     266     297 
   Size:         44     139      57      26      31

  Fragments arranged by size: 

                 139      57      44      31      26
 
HaeII r_GCGC'y

Cuts at:      0      40     297 
   Size:         40     257
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     226     258     297 
   Size:        226      32      39

  Fragments arranged by size: 

                 226      39      32
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     182     297 
   Size:        182     115
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     172     297 
   Size:        172     125
 
SmaI CCC'GGG

Cuts at:      0      10     297 
   Size:         10     287
 
StyI C'CwwG_G

Cuts at:      0     128     297 
   Size:        128     169
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     174     205     297 
   Size:        174      31      92

  Fragments arranged by size: 

                 174      92      31
 
XmaI C'CCGG_G

Cuts at:      0       8     297 
   Size:          8     289
 

 Enzymes that do cut:

     AcuI    AlwNI     AvaI     BglI     BplI     BpmI    BseYI     BsgI
  BsiHKAI Bsp1286I   BstEII   Bsu36I     BtgI     DrdI  Eco57MI    HaeII
    Hin4I    PflMI     SfiI     SmaI     StyI    TaqII     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BmtI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BsiEI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI    BstXI    BstYI  BstZ17I
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     EaeI     EagI
     EarI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfcI     SfoI     SgfI    SgrAI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmnI     ZraI