(Linear) (Six-base) MAPSORT of: KIEE3646.seq  Check: 673  from: 1  to: 585



KIEE3646.orf

 With 182 enzymes: SgfI * 

                        December 27, 2011 16:37  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     412     585 
   Size:        412     173
 
AseI AT'TA_AT

Cuts at:      0     461     585 
   Size:        461     124
 
AvaI C'yCGr_G

Cuts at:      0      34     585 
   Size:         34     551
 
BanI G'GyrC_C

Cuts at:      0     424     585 
   Size:        424     161
 
BanII G_rGCy'C

Cuts at:      0      20     476     585 
   Size:         20     456     109

  Fragments arranged by size: 

                 456     109      20
 
BlpI GC'TnA_GC

Cuts at:      0     234     585 
   Size:        234     351
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      53     585 
   Size:         53     532
 
Bpu10I CC'TnA_GC

Cuts at:      0     401     585 
   Size:        401     184
 
BsiHKAI G_wGCw'C

Cuts at:      0      20     476     585 
   Size:         20     456     109

  Fragments arranged by size: 

                 456     109      20
 
Bsp1286I G_dGCh'C

Cuts at:      0      20     476     585 
   Size:         20     456     109

  Fragments arranged by size: 

                 456     109      20
 
BsrFI r'CCGG_y

Cuts at:      0     575     585 
   Size:        575      10
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     454     585 
   Size:        454     131
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     523     585 
   Size:        523      62
 
BstYI r'GATC_y

Cuts at:      0     497     585 
   Size:        497      88
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     330     585 
   Size:        330     255
 
BtsI GCAGTG_nn'

Cuts at:      0     182     585 
   Size:        182     403
 
EaeI y'GGCC_r

Cuts at:      0     341     585 
   Size:        341     244
 
EarI CTCTTCn'nnn_

Cuts at:      0     464     585 
   Size:        464     121
 
EcoICRI GAG'CTC

Cuts at:      0      18     474     585 
   Size:         18     456     111

  Fragments arranged by size: 

                 456     111      18
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     412     585 
   Size:        412     173
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      46     585 
   Size:         46     539
 
MscI TGG'CCA

Cuts at:      0     343     585 
   Size:        343     242
 
MslI CAynn'nnrTG

Cuts at:      0     337     521     585 
   Size:        337     184      64

  Fragments arranged by size: 

                 337     184      64
 
MspA1I CmG'CkG

Cuts at:      0     238     244     585 
   Size:        238       6     341

  Fragments arranged by size: 

                 341     238       6
 
PfoI T'CCnGG_A

Cuts at:      0      80     585 
   Size:         80     505
 
PstI C_TGCA'G

Cuts at:      0     243     585 
   Size:        243     342
 
PvuII CAG'CTG

Cuts at:      0     238     244     585 
   Size:        238       6     341

  Fragments arranged by size: 

                 341     238       6
 
SacI G_AGCT'C

Cuts at:      0      20     476     585 
   Size:         20     456     109

  Fragments arranged by size: 

                 456     109      20
 
SapI GCTCTTCn'nnn_

Cuts at:      0     464     585 
   Size:        464     121
 
SfcI C'TryA_G

Cuts at:      0     239     585 
   Size:        239     346
 
SgrAI Cr'CCGG_yG

Cuts at:      0     575     585 
   Size:        575      10
 
StyI C'CwwG_G

Cuts at:      0     506     585 
   Size:        506      79
 
TatI w'GTAC_w

Cuts at:      0     221     585 
   Size:        221     364
 
XmnI GAAnn'nnTTC

Cuts at:      0     200     585 
   Size:        200     385
 

 Enzymes that do cut:

     AcuI     AseI     AvaI     BanI    BanII     BlpI     BmrI   Bpu10I
  BsiHKAI Bsp1286I    BsrFI   BstAPI    BstXI    BstYI    BtgZI     BtsI
     EaeI     EarI  EcoICRI  Eco57MI     FalI     MscI     MslI   MspA1I
     PfoI     PstI    PvuII     SacI     SapI     SfcI    SgrAI     StyI
     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI    AsiSI    AvrII     BaeI    BamHI     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII Bme1580I
    BmgBI     BmtI     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrGI
   BssHII    BssSI    BstBI   BstEII  BstZ17I   Bsu36I     BtgI     ClaI
    CspCI     DraI   DraIII     DrdI     EagI     EciI    EcoNI EcoO109I
    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII    Hin4I   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII
    SacII     SalI    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     ZraI