Restriction map for
pFN21AE3641
(Linear) (Six-base) MAPSORT of: KIEE3641.seq Check: 8520 from: 1 to: 1509
KIEE3641.orf
With 182 enzymes: SgfI *
December 27, 2011 16:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 531 1509
Size: 531 978
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 28 1509
Size: 28 1481
AgeI A'CCGG_T
Cuts at: 0 34 1509
Size: 34 1475
AhdI GACnn_n'nnGTC
Cuts at: 0 935 1248 1509
Size: 935 313 261
Fragments arranged by size:
935 313 261
AlwNI CAG_nnn'CTG
Cuts at: 0 450 482 555 636 696 717 777 900
Size: 450 32 73 81 60 21 60 123
Cuts at: 900 927 1118 1462 1509
Size: 27 191 344 47
Fragments arranged by size:
450 344 191 123 81 73 60 60
47 32 27 21
ApaI G_GGCC'C
Cuts at: 0 78 92 126 1237 1509
Size: 78 14 34 1111 272
Fragments arranged by size:
1111 272 78 34 14
ApaLI G'TGCA_C
Cuts at: 0 544 825 1509
Size: 544 281 684
Fragments arranged by size:
684 544 281
ApoI r'AATT_y
Cuts at: 0 762 1147 1509
Size: 762 385 362
Fragments arranged by size:
762 385 362
AvaI C'yCGr_G
Cuts at: 0 236 1321 1509
Size: 236 1085 188
Fragments arranged by size:
1085 236 188
BamHI G'GATC_C
Cuts at: 0 1500 1509
Size: 1500 9
BanI G'GyrC_C
Cuts at: 0 249 513 1301 1509
Size: 249 264 788 208
Fragments arranged by size:
788 264 249 208
BanII G_rGCy'C
Cuts at: 0 78 92 126 473 1237 1389 1509
Size: 78 14 34 347 764 152 120
Fragments arranged by size:
764 347 152 120 78 34 14
BbsI GAAGACnn'nnnn_
Cuts at: 0 211 1509
Size: 211 1298
BbvCI CC'TCA_GC
Cuts at: 0 386 559 1509
Size: 386 173 950
Fragments arranged by size:
950 386 173
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 819 853 1509
Size: 819 34 656
Fragments arranged by size:
819 656 34
BglI GCCn_nnn'nGGC
Cuts at: 0 328 441 496 1509
Size: 328 113 55 1013
Fragments arranged by size:
1013 328 113 55
Bme1580I G_kGCm'C
Cuts at: 0 78 92 126 186 518 548 759 829
Size: 78 14 34 60 332 30 211 70
Cuts at: 829 1237 1377 1509
Size: 408 140 132
Fragments arranged by size:
408 332 211 140 132 78 70 60
34 30 14
BmrI ACTGGGnnnn_n'
Cuts at: 0 993 1443 1509
Size: 993 450 66
Fragments arranged by size:
993 450 66
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 878 993 1509
Size: 878 115 516
Fragments arranged by size:
878 516 115
Bpu10I CC'TnA_GC
Cuts at: 0 386 559 889 1509
Size: 386 173 330 620
Fragments arranged by size:
620 386 330 173
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 634 1509
Size: 634 875
BsaI GGTCTCn'nnnn_
Cuts at: 0 1321 1509
Size: 1321 188
BsaAI yAC'GTr
Cuts at: 0 1191 1509
Size: 1191 318
BsaHI Gr'CG_yC
Cuts at: 0 1184 1509
Size: 1184 325
BsaWI w'CCGG_w
Cuts at: 0 34 1509
Size: 34 1475
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 181 481 523 1339 1509
Size: 181 300 42 816 170
Fragments arranged by size:
816 300 181 170 42
BseYI C'CCAG_C
Cuts at: 0 45 98 218 804 840 1509
Size: 45 53 120 586 36 669
Fragments arranged by size:
669 586 120 53 45 36
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1324 1509
Size: 1324 185
BsiHKAI G_wGCw'C
Cuts at: 0 473 548 829 1509
Size: 473 75 281 680
Fragments arranged by size:
680 473 281 75
BsmI GAATG_Cn'
Cuts at: 0 614 1174 1509
Size: 614 560 335
Fragments arranged by size:
614 560 335
Bsp1286I G_dGCh'C
Cuts at: 0 78 92 126 186 473 518 548 759
Size: 78 14 34 60 287 45 30 211
Cuts at: 759 829 1237 1377 1389 1509
Size: 70 408 140 12 120
Fragments arranged by size:
408 287 211 140 120 78 70 60
45 34 30 14 12
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 531 644 908 1509
Size: 531 113 264 601
Fragments arranged by size:
601 531 264 113
BsrBI CCG'CTC
Cuts at: 0 233 408 1509
Size: 233 175 1101
Fragments arranged by size:
1101 233 175
BsrFI r'CCGG_y
Cuts at: 0 34 1304 1465 1509
Size: 34 1270 161 44
Fragments arranged by size:
1270 161 44 34
BstAPI GCAn_nnn'nTGC
Cuts at: 0 696 705 1462 1509
Size: 696 9 757 47
Fragments arranged by size:
757 696 47 9
BstEII G'GTnAC_C
Cuts at: 0 1020 1509
Size: 1020 489
BstXI CCAn_nnnn'nTGG
Cuts at: 0 58 1509
Size: 58 1451
BstYI r'GATC_y
Cuts at: 0 587 1500 1509
Size: 587 913 9
Fragments arranged by size:
913 587 9
BtgI C'CryG_G
Cuts at: 0 671 809 1509
Size: 671 138 700
Fragments arranged by size:
700 671 138
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 638 1377 1509
Size: 638 739 132
Fragments arranged by size:
739 638 132
BtsI GCAGTG_nn'
Cuts at: 0 1205 1460 1509
Size: 1205 255 49
Fragments arranged by size:
1205 255 49
DraIII CAC_nnn'GTG
Cuts at: 0 1020 1509
Size: 1020 489
EaeI y'GGCC_r
Cuts at: 0 275 320 578 1509
Size: 275 45 258 931
Fragments arranged by size:
931 275 258 45
EarI CTCTTCn'nnn_
Cuts at: 0 458 1414 1509
Size: 458 956 95
Fragments arranged by size:
956 458 95
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 403 1509
Size: 403 1106
EcoICRI GAG'CTC
Cuts at: 0 471 1509
Size: 471 1038
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 28 878 993 1509
Size: 28 850 115 516
Fragments arranged by size:
850 516 115 28
EcoNI CCTnn'n_nnAGG
Cuts at: 0 152 163 892 1411 1509
Size: 152 11 729 519 98
Fragments arranged by size:
729 519 152 98 11
EcoO109I rG'GnC_Cy
Cuts at: 0 18 74 75 82 89 552 801 864
Size: 18 56 1 7 7 463 249 63
Cuts at: 864 885 897 1140 1233 1234 1278 1404 1509
Size: 21 12 243 93 1 44 126 105
Fragments arranged by size:
463 249 243 126 105 93 63 56
44 21 18 12 7 7 1 1
HaeII r_GCGC'y
Cuts at: 0 425 1509
Size: 425 1084
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 921 953 1106 1138 1234 1266 1376 1408
Size: 921 32 153 32 96 32 110 32
Cuts at: 1408 1509
Size: 101
Fragments arranged by size:
921 153 110 101 96 32 32 32
32
MfeI C'AATT_G
Cuts at: 0 533 1509
Size: 533 976
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1205 1509
Size: 1205 304
MscI TGG'CCA
Cuts at: 0 277 1509
Size: 277 1232
MslI CAynn'nnrTG
Cuts at: 0 1084 1288 1509
Size: 1084 204 221
Fragments arranged by size:
1084 221 204
MspA1I CmG'CkG
Cuts at: 0 45 98 222 390 440 526 673 774
Size: 45 53 124 168 50 86 147 101
Cuts at: 774 1102 1509
Size: 328 407
Fragments arranged by size:
407 328 168 147 124 101 86 53
50 45
NaeI GCC'GGC
Cuts at: 0 1467 1509
Size: 1467 42
NgoMIV G'CCGG_C
Cuts at: 0 1465 1509
Size: 1465 44
PasI CC'CwG_GG
Cuts at: 0 78 1230 1509
Size: 78 1152 279
Fragments arranged by size:
1152 279 78
PfoI T'CCnGG_A
Cuts at: 0 1412 1509
Size: 1412 97
PmlI CAC'GTG
Cuts at: 0 1191 1509
Size: 1191 318
PpuMI rG'GwC_Cy
Cuts at: 0 801 864 897 1140 1278 1404 1509
Size: 801 63 33 243 138 126 105
Fragments arranged by size:
801 243 138 126 105 63 33
PshAI GACnn'nnGTC
Cuts at: 0 737 1509
Size: 737 772
PspOMI G'GGCC_C
Cuts at: 0 74 88 122 1233 1509
Size: 74 14 34 1111 276
Fragments arranged by size:
1111 276 74 34 14
PstI C_TGCA'G
Cuts at: 0 362 395 701 1101 1130 1402 1509
Size: 362 33 306 400 29 272 107
Fragments arranged by size:
400 362 306 272 107 33 29
PvuII CAG'CTG
Cuts at: 0 45 222 390 774 1102 1509
Size: 45 177 168 384 328 407
Fragments arranged by size:
407 384 328 177 168 45
SacI G_AGCT'C
Cuts at: 0 473 1509
Size: 473 1036
SacII CC_GC'GG
Cuts at: 0 674 1509
Size: 674 835
SanDI GG'GwC_CC
Cuts at: 0 801 864 1509
Size: 801 63 645
Fragments arranged by size:
801 645 63
SbfI CC_TGCA'GG
Cuts at: 0 701 1402 1509
Size: 701 701 107
Fragments arranged by size:
701 701 107
ScaI AGT'ACT
Cuts at: 0 317 1509
Size: 317 1192
SexAI A'CCwGG_T
Cuts at: 0 1016 1509
Size: 1016 493
SfcI C'TryA_G
Cuts at: 0 358 391 697 1097 1126 1398 1509
Size: 358 33 306 400 29 272 111
Fragments arranged by size:
400 358 306 272 111 33 29
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 496 1509
Size: 496 1013
SmlI C'TyrA_G
Cuts at: 0 236 649 1509
Size: 236 413 860
Fragments arranged by size:
860 413 236
StuI AGG'CCT
Cuts at: 0 158 703 1509
Size: 158 545 806
Fragments arranged by size:
806 545 158
StyI C'CwwG_G
Cuts at: 0 491 503 1169 1509
Size: 491 12 666 340
Fragments arranged by size:
666 491 340 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 151 1243 1485 1509
Size: 151 1092 242 24
Fragments arranged by size:
1092 242 151 24
TatI w'GTAC_w
Cuts at: 0 315 1509
Size: 315 1194
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 281 313 1509
Size: 281 32 1196
Fragments arranged by size:
1196 281 32
Tth111I GACn'n_nGTC
Cuts at: 0 1120 1509
Size: 1120 389
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 145 1509
Size: 145 1364
XhoI C'TCGA_G
Cuts at: 0 236 1509
Size: 236 1273
Enzymes that do cut:
AarI AcuI AgeI AhdI AlwNI ApaI ApaLI ApoI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BglI
Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI
BsaWI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI
BsrBI BsrFI BstAPI BstEII BstXI BstYI BtgI BtgZI
BtsI DraIII EaeI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I HaeII Hin4I MfeI MmeI MscI MslI MspA1I
NaeI NgoMIV PasI PfoI PmlI PpuMI PshAI PspOMI
PstI PvuII SacI SacII SanDI SbfI ScaI SexAI
SfcI SfiI SmlI StuI StyI TaqII TatI TstI
Tth111I XcmI XhoI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AleI
AlfI AloI AscI AseI AsiSI AvrII BaeI BbeI
BciVI BclI BfrBI BglII BlpI BmgBI BmtI BplI
BsaBI BsaXI BsiEI BsiWI BsmBI BspEI BspHI BsrDI
BsrGI BssHII BssSI BstBI BstZ17I Bsu36I ClaI CspCI
DraI DrdI EagI EcoRI EcoRV FalI FseI FspI
FspAI HincII HindIII HpaI KasI KpnI MluI NarI
NcoI NdeI NheI NotI NruI NsiI NspI PacI
PciI PflMI PmeI PpiI PsiI PspXI PsrI PvuI
RsrII SalI SapI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI SwaI XbaI XmaI XmnI
ZraI