Restriction map for
pFN21AE3640
(Linear) (Six-base) MAPSORT of: KIEE3640.seq Check: 7257 from: 1 to: 2466
KIEE3640.orf
With 182 enzymes: SgfI *
December 27, 2011 16:37 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 191 203 1445 2466
Size: 191 12 1242 1021
Fragments arranged by size:
1242 1021 191 12
AflIII A'CryG_T
Cuts at: 0 607 2394 2466
Size: 607 1787 72
Fragments arranged by size:
1787 607 72
AleI CACnn'nnGTG
Cuts at: 0 2395 2466
Size: 2395 71
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2291 2466
Size: 2291 175
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 14 46 2466
Size: 14 32 2420
Fragments arranged by size:
2420 32 14
AlwNI CAG_nnn'CTG
Cuts at: 0 903 1840 2466
Size: 903 937 626
Fragments arranged by size:
937 903 626
ApoI r'AATT_y
Cuts at: 0 252 558 1373 1662 1734 2466
Size: 252 306 815 289 72 732
Fragments arranged by size:
815 732 306 289 252 72
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2422 2455 2466
Size: 2422 33 11
Fragments arranged by size:
2422 33 11
BanI G'GyrC_C
Cuts at: 0 1672 2466
Size: 1672 794
BbsI GAAGACnn'nnnn_
Cuts at: 0 1368 1433 2466
Size: 1368 65 1033
Fragments arranged by size:
1368 1033 65
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1932 1966 2466
Size: 1932 34 500
Fragments arranged by size:
1932 500 34
BclI T'GATC_A
Cuts at: 0 1094 1104 1170 2466
Size: 1094 10 66 1296
Fragments arranged by size:
1296 1094 66 10
BfrBI ATG'CAT
Cuts at: 0 1509 2466
Size: 1509 957
BglI GCCn_nnn'nGGC
Cuts at: 0 239 2466
Size: 239 2227
BglII A'GATC_T
Cuts at: 0 295 2466
Size: 295 2171
BmrI ACTGGGnnnn_n'
Cuts at: 0 1153 2466
Size: 1153 1313
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 354 740 1654 2466
Size: 354 386 914 812
Fragments arranged by size:
914 812 386 354
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 769 2466
Size: 769 1697
BsaI GGTCTCn'nnnn_
Cuts at: 0 799 2466
Size: 799 1667
BsaAI yAC'GTr
Cuts at: 0 351 2466
Size: 351 2115
BsaBI GATnn'nnATC
Cuts at: 0 1202 1229 1628 2466
Size: 1202 27 399 838
Fragments arranged by size:
1202 838 399 27
BsaHI Gr'CG_yC
Cuts at: 0 289 2466
Size: 289 2177
BsaWI w'CCGG_w
Cuts at: 0 2145 2466
Size: 2145 321
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1635 1665 1965 1995 2466
Size: 1635 30 300 30 471
Fragments arranged by size:
1635 471 300 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2054 2466
Size: 2054 412
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 90 2466
Size: 90 2376
BsmI GAATG_Cn'
Cuts at: 0 1746 2466
Size: 1746 720
BspEI T'CCGG_A
Cuts at: 0 2145 2466
Size: 2145 321
BspHI T'CATG_A
Cuts at: 0 1131 2466
Size: 1131 1335
BsrDI GCAATG_nn'
Cuts at: 0 1701 2466
Size: 1701 765
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2406 2466
Size: 2406 60
BstYI r'GATC_y
Cuts at: 0 295 1830 2466
Size: 295 1535 636
Fragments arranged by size:
1535 636 295
BtsI GCAGTG_nn'
Cuts at: 0 869 2273 2377 2466
Size: 869 1404 104 89
Fragments arranged by size:
1404 869 104 89
ClaI AT'CG_AT
Cuts at: 0 38 2466
Size: 38 2428
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 698 733 2466
Size: 698 35 1733
Fragments arranged by size:
1733 698 35
DraI TTT'AAA
Cuts at: 0 873 1397 1667 2087 2466
Size: 873 524 270 420 379
Fragments arranged by size:
873 524 420 379 270
EaeI y'GGCC_r
Cuts at: 0 231 1545 2466
Size: 231 1314 921
Fragments arranged by size:
1314 921 231
EarI CTCTTCn'nnn_
Cuts at: 0 1754 2084 2466
Size: 1754 330 382
Fragments arranged by size:
1754 382 330
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 191 203 354 740 1445 1654 2466
Size: 191 12 151 386 705 209 812
Fragments arranged by size:
812 705 386 209 191 151 12
EcoO109I rG'GnC_Cy
Cuts at: 0 760 2466
Size: 760 1706
EcoRI G'AATT_C
Cuts at: 0 558 2466
Size: 558 1908
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 982 2466
Size: 982 1484
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 31 63 2466
Size: 31 32 2403
Fragments arranged by size:
2403 32 31
HincII GTy'rAC
Cuts at: 0 1062 1527 2127 2466
Size: 1062 465 600 339
Fragments arranged by size:
1062 600 465 339
HpaI GTT'AAC
Cuts at: 0 2127 2466
Size: 2127 339
MfeI C'AATT_G
Cuts at: 0 692 2466
Size: 692 1774
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 977 1505 1920 2466
Size: 977 528 415 546
Fragments arranged by size:
977 546 528 415
MscI TGG'CCA
Cuts at: 0 233 1547 2466
Size: 233 1314 919
Fragments arranged by size:
1314 919 233
MslI CAynn'nnrTG
Cuts at: 0 1397 1757 2395 2466
Size: 1397 360 638 71
Fragments arranged by size:
1397 638 360 71
NsiI A_TGCA'T
Cuts at: 0 1511 2466
Size: 1511 955
NspI r_CATG'y
Cuts at: 0 271 424 611 2023 2398 2466
Size: 271 153 187 1412 375 68
Fragments arranged by size:
1412 375 271 187 153 68
PciI A'CATG_T
Cuts at: 0 607 2394 2466
Size: 607 1787 72
Fragments arranged by size:
1787 607 72
PfoI T'CCnGG_A
Cuts at: 0 1797 2466
Size: 1797 669
SapI GCTCTTCn'nnn_
Cuts at: 0 1754 2466
Size: 1754 712
ScaI AGT'ACT
Cuts at: 0 2423 2466
Size: 2423 43
SmlI C'TyrA_G
Cuts at: 0 748 2466
Size: 748 1718
SnaBI TAC'GTA
Cuts at: 0 351 2466
Size: 351 2115
SpeI A'CTAG_T
Cuts at: 0 1087 2466
Size: 1087 1379
SphI G_CATG'C
Cuts at: 0 271 2466
Size: 271 2195
SspI AAT'ATT
Cuts at: 0 465 1473 1519 2466
Size: 465 1008 46 947
Fragments arranged by size:
1008 947 465 46
StyI C'CwwG_G
Cuts at: 0 1124 2285 2466
Size: 1124 1161 181
Fragments arranged by size:
1161 1124 181
SwaI ATTT'AAAT
Cuts at: 0 1397 1667 2466
Size: 1397 270 799
Fragments arranged by size:
1397 799 270
TatI w'GTAC_w
Cuts at: 0 1408 1849 2421 2466
Size: 1408 441 572 45
Fragments arranged by size:
1408 572 441 45
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1793 1825 2466
Size: 1793 32 641
Fragments arranged by size:
1793 641 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 241 2466
Size: 241 2225
Enzymes that do cut:
AcuI AflIII AleI AlfI AloI AlwNI ApoI BaeI
BanI BbsI BcgI BclI BfrBI BglI BglII BmrI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BsgI BsmI BspEI BspHI BsrDI BstAPI BstYI
BtsI ClaI CspCI DraI EaeI EarI Eco57MI EcoO109I
EcoRI FalI Hin4I HincII HpaI MfeI MmeI MscI
MslI NsiI NspI PciI PfoI SapI ScaI SmlI
SnaBI SpeI SphI SspI StyI SwaI TatI TstI
XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI ApaI ApaLI AscI AseI AsiSI AvaI AvrII
BamHI BanII BbeI BbvCI BciVI BlpI Bme1580I BmgBI
BmtI BplI Bpu10I BseYI BsiEI BsiHKAI BsiWI BsmBI
Bsp1286I BspMI BsrBI BsrFI BsrGI BssHII BssSI BstBI
BstEII BstXI BstZ17I Bsu36I BtgI BtgZI DraIII DrdI
EagI EciI EcoICRI EcoNI EcoRV FseI FspI FspAI
HaeII HindIII KasI KpnI MluI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI PacI PasI
PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SbfI SexAI SfcI SfiI SfoI SgfI
SgrAI SmaI SrfI StuI TaqII Tth111I XbaI XhoI
XmaI XmnI ZraI