Restriction map for
pFN21AE3638
(Linear) (Six-base) MAPSORT of: KIEE3638.seq Check: 8259 from: 1 to: 1278
KIEE3638.orf
With 182 enzymes: SgfI *
December 27, 2011 16:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 768 1171 1278
Size: 768 403 107
Fragments arranged by size:
768 403 107
Acc65I G'GTAC_C
Cuts at: 0 890 1278
Size: 890 388
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 488 680 1278
Size: 488 192 598
Fragments arranged by size:
598 488 192
AleI CACnn'nnGTG
Cuts at: 0 1189 1278
Size: 1189 89
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 133 165 492 524 1278
Size: 133 32 327 32 754
Fragments arranged by size:
754 327 133 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1045 1236 1278
Size: 1045 191 42
Fragments arranged by size:
1045 191 42
ApaI G_GGCC'C
Cuts at: 0 916 1278
Size: 916 362
ApoI r'AATT_y
Cuts at: 0 181 445 604 1278
Size: 181 264 159 674
Fragments arranged by size:
674 264 181 159
BanI G'GyrC_C
Cuts at: 0 642 890 1093 1117 1278
Size: 642 248 203 24 161
Fragments arranged by size:
642 248 203 161 24
BanII G_rGCy'C
Cuts at: 0 7 35 62 916 941 1229 1278
Size: 7 28 27 854 25 288 49
Fragments arranged by size:
854 288 49 28 27 25 7
BbeI G_GCGC'C
Cuts at: 0 1121 1278
Size: 1121 157
BbsI GAAGACnn'nnnn_
Cuts at: 0 239 1278
Size: 239 1039
BbvCI CC'TCA_GC
Cuts at: 0 1046 1278
Size: 1046 232
BglI GCCn_nnn'nGGC
Cuts at: 0 16 1278
Size: 16 1262
Bme1580I G_kGCm'C
Cuts at: 0 86 673 916 1278
Size: 86 587 243 362
Fragments arranged by size:
587 362 243 86
BmrI ACTGGGnnnn_n'
Cuts at: 0 186 399 1278
Size: 186 213 879
Fragments arranged by size:
879 213 186
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 1213 1278
Size: 76 1137 65
Fragments arranged by size:
1137 76 65
Bpu10I CC'TnA_GC
Cuts at: 0 1046 1278
Size: 1046 232
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 646 907 925 966 1278
Size: 646 261 18 41 312
Fragments arranged by size:
646 312 261 41 18
BsaI GGTCTCn'nnnn_
Cuts at: 0 151 1278
Size: 151 1127
BsaHI Gr'CG_yC
Cuts at: 0 47 1118 1278
Size: 47 1071 160
Fragments arranged by size:
1071 160 47
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 970 1000 1048 1078 1278
Size: 970 30 48 30 200
Fragments arranged by size:
970 200 48 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 427 715 1278
Size: 427 288 563
Fragments arranged by size:
563 427 288
BseYI C'CCAG_C
Cuts at: 0 6 512 1278
Size: 6 506 766
Fragments arranged by size:
766 506 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 865 1278
Size: 865 413
BsiHKAI G_wGCw'C
Cuts at: 0 98 742 923 941 1278
Size: 98 644 181 18 337
Fragments arranged by size:
644 337 181 98 18
BsmI GAATG_Cn'
Cuts at: 0 585 1278
Size: 585 693
Bsp1286I G_dGCh'C
Cuts at: 0 7 35 62 86 98 673 742 916
Size: 7 28 27 24 12 575 69 174
Cuts at: 916 923 941 1229 1278
Size: 7 18 288 49
Fragments arranged by size:
575 288 174 69 49 28 27 24
18 12 7 7
BspHI T'CATG_A
Cuts at: 0 812 1278
Size: 812 466
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 768 902 1171 1278
Size: 768 134 269 107
Fragments arranged by size:
768 269 134 107
BsrBI CCG'CTC
Cuts at: 0 1017 1278
Size: 1017 261
BstXI CCAn_nnnn'nTGG
Cuts at: 0 27 1210 1278
Size: 27 1183 68
Fragments arranged by size:
1183 68 27
BstYI r'GATC_y
Cuts at: 0 719 1278
Size: 719 559
Bsu36I CC'TnA_GG
Cuts at: 0 331 383 1278
Size: 331 52 895
Fragments arranged by size:
895 331 52
BtgI C'CryG_G
Cuts at: 0 20 276 825 1187 1278
Size: 20 256 549 362 91
Fragments arranged by size:
549 362 256 91 20
BtsI GCAGTG_nn'
Cuts at: 0 801 953 1278
Size: 801 152 325
Fragments arranged by size:
801 325 152
EarI CTCTTCn'nnn_
Cuts at: 0 418 1278
Size: 418 860
EcoICRI GAG'CTC
Cuts at: 0 939 1278
Size: 939 339
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 488 680 1213 1278
Size: 76 412 192 533 65
Fragments arranged by size:
533 412 192 76 65
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1066 1278
Size: 1066 212
EcoO109I rG'GnC_Cy
Cuts at: 0 913 1059 1278
Size: 913 146 219
Fragments arranged by size:
913 219 146
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 67 1278
Size: 67 1211
FspI TGC'GCA
Cuts at: 0 178 1138 1278
Size: 178 960 140
Fragments arranged by size:
960 178 140
FspAI rTGC'GCAy
Cuts at: 0 1138 1278
Size: 1138 140
HaeII r_GCGC'y
Cuts at: 0 1121 1278
Size: 1121 157
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1048 1080 1278
Size: 1048 32 198
Fragments arranged by size:
1048 198 32
KasI G'GCGC_C
Cuts at: 0 1117 1278
Size: 1117 161
KpnI G_GTAC'C
Cuts at: 0 894 1278
Size: 894 384
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 932 1278
Size: 932 346
MslI CAynn'nnrTG
Cuts at: 0 25 686 757 778 811 1189 1278
Size: 25 661 71 21 33 378 89
Fragments arranged by size:
661 378 89 71 33 25 21
MspA1I CmG'CkG
Cuts at: 0 1078 1278
Size: 1078 200
NarI GG'CG_CC
Cuts at: 0 1118 1278
Size: 1118 160
NcoI C'CATG_G
Cuts at: 0 20 276 825 1278
Size: 20 256 549 453
Fragments arranged by size:
549 453 256 20
NspI r_CATG'y
Cuts at: 0 777 1278
Size: 777 501
PfoI T'CCnGG_A
Cuts at: 0 1031 1278
Size: 1031 247
PpuMI rG'GwC_Cy
Cuts at: 0 1059 1278
Size: 1059 219
PshAI GACnn'nnGTC
Cuts at: 0 963 1278
Size: 963 315
PspOMI G'GGCC_C
Cuts at: 0 912 1278
Size: 912 366
PstI C_TGCA'G
Cuts at: 0 750 1168 1278
Size: 750 418 110
Fragments arranged by size:
750 418 110
PvuII CAG'CTG
Cuts at: 0 1078 1278
Size: 1078 200
SacI G_AGCT'C
Cuts at: 0 941 1278
Size: 941 337
SapI GCTCTTCn'nnn_
Cuts at: 0 418 1278
Size: 418 860
SbfI CC_TGCA'GG
Cuts at: 0 1168 1278
Size: 1168 110
SexAI A'CCwGG_T
Cuts at: 0 71 1278
Size: 71 1207
SfcI C'TryA_G
Cuts at: 0 485 746 1127 1164 1278
Size: 485 261 381 37 114
Fragments arranged by size:
485 381 261 114 37
SfoI GGC'GCC
Cuts at: 0 1119 1278
Size: 1119 159
SmlI C'TyrA_G
Cuts at: 0 661 922 940 981 1278
Size: 661 261 18 41 297
Fragments arranged by size:
661 297 261 41 18
SphI G_CATG'C
Cuts at: 0 777 1278
Size: 777 501
StyI C'CwwG_G
Cuts at: 0 20 136 276 532 825 1278
Size: 20 116 140 256 293 453
Fragments arranged by size:
453 293 256 140 116 20
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 381 1278
Size: 381 897
TatI w'GTAC_w
Cuts at: 0 194 476 599 1278
Size: 194 282 123 679
Fragments arranged by size:
679 282 194 123
Tth111I GACn'n_nGTC
Cuts at: 0 1246 1278
Size: 1246 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 793 1278
Size: 793 485
Enzymes that do cut:
AarI Acc65I AcuI AleI AloI AlwNI ApaI ApoI
BanI BanII BbeI BbsI BbvCI BglI Bme1580I BmrI
BpmI Bpu10I BpuEI BsaI BsaHI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BstXI
BstYI Bsu36I BtgI BtsI EarI EcoICRI Eco57MI EcoNI
EcoO109I FalI FspI FspAI HaeII Hin4I KasI KpnI
MmeI MslI MspA1I NarI NcoI NspI PfoI PpuMI
PshAI PspOMI PstI PvuII SacI SapI SbfI SexAI
SfcI SfoI SmlI SphI StyI TaqII TatI Tth111I
XcmI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AflIII AgeI AhdI
AlfI ApaLI AscI AseI AsiSI AvaI AvrII BaeI
BamHI BcgI BciVI BclI BfrBI BglII BlpI BmgBI
BmtI BplI BsaAI BsaBI BsaWI BsiEI BsiWI BsmBI
BspEI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstZ17I BtgZI ClaI CspCI DraI DraIII DrdI
EaeI EagI EciI EcoRI EcoRV FseI HincII HindIII
HpaI MfeI MluI MscI NaeI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PciI PflMI PmeI
PmlI PpiI PsiI PspXI PsrI PvuI RsrII SacII
SalI SanDI ScaI SfiI SgfI SgrAI SmaI SnaBI
SpeI SrfI SspI StuI SwaI TstI XbaI XhoI
XmaI XmnI ZraI