(Linear) (Six-base) MAPSORT of: KIEE3636.seq  Check: 3456  from: 1  to: 552



KIEE3636.orf

 With 182 enzymes: SgfI * 

                        December 27, 2011 16:36  ..

AflIII A'CryG_T

Cuts at:      0     239     552 
   Size:        239     313
 
ApoI r'AATT_y

Cuts at:      0     184     552 
   Size:        184     368
 
BanI G'GyrC_C

Cuts at:      0     165     552 
   Size:        165     387
 
Bme1580I G_kGCm'C

Cuts at:      0     168     533     552 
   Size:        168     365      19

  Fragments arranged by size: 

                 365     168      19
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     527     552 
   Size:        527      25
 
BmtI G_CTAG'C

Cuts at:      0       8     552 
   Size:          8     544
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     124     552 
   Size:        124     428
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      82     552 
   Size:         82     470
 
BsaAI yAC'GTr

Cuts at:      0     240     552 
   Size:        240     312
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     466     496     552 
   Size:        466      30      56

  Fragments arranged by size: 

                 466      56      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0       9     100     376     529     532     535     552 
   Size:          9      91     276     153       3       3      17

  Fragments arranged by size: 

                 276     153      91      17       9       3       3
 
BseYI C'CCAG_C

Cuts at:      0       9     214     552 
   Size:          9     205     338

  Fragments arranged by size: 

                 338     205       9
 
BsmI GAATG_Cn'

Cuts at:      0      81     552 
   Size:         81     471
 
Bsp1286I G_dGCh'C

Cuts at:      0     168     533     552 
   Size:        168     365      19

  Fragments arranged by size: 

                 365     168      19
 
BspHI T'CATG_A

Cuts at:      0     113     552 
   Size:        113     439
 
BsrBI CCG'CTC

Cuts at:      0     108     552 
   Size:        108     444
 
BsrFI r'CCGG_y

Cuts at:      0     220     552 
   Size:        220     332
 
BsrGI T'GTAC_A

Cuts at:      0     242     552 
   Size:        242     310
 
BtgI C'CryG_G

Cuts at:      0     293     542     552 
   Size:        293     249      10

  Fragments arranged by size: 

                 293     249      10
 
BtsI GCAGTG_nn'

Cuts at:      0     231     552 
   Size:        231     321
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      97     552 
   Size:         97     455
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     124     552 
   Size:        124     428
 
EcoO109I rG'GnC_Cy

Cuts at:      0     457     552 
   Size:        457      95
 
HaeII r_GCGC'y

Cuts at:      0     381     393     552 
   Size:        381      12     159

  Fragments arranged by size: 

                 381     159      12
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     252     284     466     498     552 
   Size:        252      32     182      32      54

  Fragments arranged by size: 

                 252     182      54      32      32
 
NaeI GCC'GGC

Cuts at:      0     222     552 
   Size:        222     330
 
NcoI C'CATG_G

Cuts at:      0     293     542     552 
   Size:        293     249      10

  Fragments arranged by size: 

                 293     249      10
 
NgoMIV G'CCGG_C

Cuts at:      0     220     552 
   Size:        220     332
 
NheI G'CTAG_C

Cuts at:      0       4     552 
   Size:          4     548
 
PmlI CAC'GTG

Cuts at:      0     240     552 
   Size:        240     312
 
PpuMI rG'GwC_Cy

Cuts at:      0     457     552 
   Size:        457      95
 
PshAI GACnn'nnGTC

Cuts at:      0      61     552 
   Size:         61     491
 
PstI C_TGCA'G

Cuts at:      0     360     552 
   Size:        360     192
 
SfcI C'TryA_G

Cuts at:      0     356     552 
   Size:        356     196
 
SmlI C'TyrA_G

Cuts at:      0      97     552 
   Size:         97     455
 
StyI C'CwwG_G

Cuts at:      0     293     470     542     552 
   Size:        293     177      72      10

  Fragments arranged by size: 

                 293     177      72      10
 
TatI w'GTAC_w

Cuts at:      0     242     552 
   Size:        242     310
 
Tth111I GACn'n_nGTC

Cuts at:      0     461     552 
   Size:        461      91
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     540     552 
   Size:        540      12
 

 Enzymes that do cut:

   AflIII     ApoI     BanI Bme1580I     BmrI     BmtI     BpmI    BpuEI
    BsaAI    BsaXI    BseRI    BseYI     BsmI Bsp1286I    BspHI    BsrBI
    BsrFI    BsrGI     BtgI     BtsI     EciI  Eco57MI EcoO109I    HaeII
    Hin4I     NaeI     NcoI   NgoMIV     NheI     PmlI    PpuMI    PshAI
     PstI     SfcI     SmlI     StyI     TatI  Tth111I     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI    BmgBI     BplI   Bpu10I     BsaI    BsaBI    BsaHI
    BsaWI     BsgI    BsiEI  BsiHKAI    BsiWI    BsmBI    BspEI    BspMI
    BsrDI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI
  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI     EarI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NarI     NdeI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PpiI     PsiI   PspOMI    PspXI     PsrI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII     TstI
     XbaI     XhoI     XmaI     XmnI     ZraI