Restriction map for
pFN21AE3635
(Linear) (Six-base) MAPSORT of: KIEE3635.seq Check: 7441 from: 1 to: 1386
KIEE3635.orf
With 182 enzymes: SgfI *
December 27, 2011 16:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1023 1386
Size: 1023 363
AatII G_ACGT'C
Cuts at: 0 1352 1386
Size: 1352 34
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 117 1386
Size: 117 1269
AfeI AGC'GCT
Cuts at: 0 1357 1386
Size: 1357 29
AflIII A'CryG_T
Cuts at: 0 118 1386
Size: 118 1268
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 70 1386
Size: 70 1316
AlwNI CAG_nnn'CTG
Cuts at: 0 609 1236 1386
Size: 609 627 150
Fragments arranged by size:
627 609 150
ApoI r'AATT_y
Cuts at: 0 61 232 1386
Size: 61 171 1154
Fragments arranged by size:
1154 171 61
AvaI C'yCGr_G
Cuts at: 0 425 1040 1386
Size: 425 615 346
Fragments arranged by size:
615 425 346
BanI G'GyrC_C
Cuts at: 0 107 1386
Size: 107 1279
BanII G_rGCy'C
Cuts at: 0 82 143 314 496 551 1116 1386
Size: 82 61 171 182 55 565 270
Fragments arranged by size:
565 270 182 171 82 61 55
BbsI GAAGACnn'nnnn_
Cuts at: 0 123 1386
Size: 123 1263
BbvCI CC'TCA_GC
Cuts at: 0 535 1386
Size: 535 851
BlpI GC'TnA_GC
Cuts at: 0 268 527 629 718 1386
Size: 268 259 102 89 668
Fragments arranged by size:
668 268 259 102 89
Bme1580I G_kGCm'C
Cuts at: 0 110 898 1343 1386
Size: 110 788 445 43
Fragments arranged by size:
788 445 110 43
BmrI ACTGGGnnnn_n'
Cuts at: 0 171 494 1114 1386
Size: 171 323 620 272
Fragments arranged by size:
620 323 272 171
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 717 785 848 1386
Size: 717 68 63 538
Fragments arranged by size:
717 538 68 63
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 205 298 590 905 1386
Size: 205 93 292 315 481
Fragments arranged by size:
481 315 292 205 93
Bpu10I CC'TnA_GC
Cuts at: 0 76 245 535 1386
Size: 76 169 290 851
Fragments arranged by size:
851 290 169 76
BsaHI Gr'CG_yC
Cuts at: 0 1349 1386
Size: 1349 37
BsaWI w'CCGG_w
Cuts at: 0 278 1202 1386
Size: 278 924 184
Fragments arranged by size:
924 278 184
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 284 314 421 451 1386
Size: 284 30 107 30 935
Fragments arranged by size:
935 284 107 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 472 523 1386
Size: 472 51 863
Fragments arranged by size:
863 472 51
BseYI C'CCAG_C
Cuts at: 0 405 544 889 1082 1281 1386
Size: 405 139 345 193 199 105
Fragments arranged by size:
405 345 199 193 139 105
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 524 1072 1386
Size: 524 548 314
Fragments arranged by size:
548 524 314
BsiEI CG_ry'CG
Cuts at: 0 1337 1386
Size: 1337 49
BsiHKAI G_wGCw'C
Cuts at: 0 10 143 1292 1386
Size: 10 133 1149 94
Fragments arranged by size:
1149 133 94 10
Bsp1286I G_dGCh'C
Cuts at: 0 10 82 110 143 314 496 551 898
Size: 10 72 28 33 171 182 55 347
Cuts at: 898 1116 1292 1343 1386
Size: 218 176 51 43
Fragments arranged by size:
347 218 182 176 171 72 55 51
43 33 28 10
BspEI T'CCGG_A
Cuts at: 0 278 1386
Size: 278 1108
BspHI T'CATG_A
Cuts at: 0 551 1386
Size: 551 835
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1023 1386
Size: 1023 363
BsrBI CCG'CTC
Cuts at: 0 272 1386
Size: 272 1114
BsrDI GCAATG_nn'
Cuts at: 0 356 980 1386
Size: 356 624 406
Fragments arranged by size:
624 406 356
BssSI C'ACGA_G
Cuts at: 0 1291 1386
Size: 1291 95
BstAPI GCAn_nnn'nTGC
Cuts at: 0 195 657 792 1386
Size: 195 462 135 594
Fragments arranged by size:
594 462 195 135
BstEII G'GTnAC_C
Cuts at: 0 1035 1326 1386
Size: 1035 291 60
Fragments arranged by size:
1035 291 60
BstXI CCAn_nnnn'nTGG
Cuts at: 0 123 855 1023 1386
Size: 123 732 168 363
Fragments arranged by size:
732 363 168 123
BstYI r'GATC_y
Cuts at: 0 281 1169 1386
Size: 281 888 217
Fragments arranged by size:
888 281 217
Bsu36I CC'TnA_GG
Cuts at: 0 1102 1386
Size: 1102 284
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 477 1386
Size: 477 909
DraIII CAC_nnn'GTG
Cuts at: 0 1122 1386
Size: 1122 264
EaeI y'GGCC_r
Cuts at: 0 857 1307 1334 1386
Size: 857 450 27 52
Fragments arranged by size:
857 450 52 27
EagI C'GGCC_G
Cuts at: 0 1334 1386
Size: 1334 52
EarI CTCTTCn'nnn_
Cuts at: 0 550 715 817 994 1386
Size: 550 165 102 177 392
Fragments arranged by size:
550 392 177 165 102
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 331 1386
Size: 331 1055
EcoICRI GAG'CTC
Cuts at: 0 141 1386
Size: 141 1245
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 117 205 298 590 905 1386
Size: 117 88 93 292 315 481
Fragments arranged by size:
481 315 292 117 93 88
EcoNI CCTnn'n_nnAGG
Cuts at: 0 538 1386
Size: 538 848
EcoO109I rG'GnC_Cy
Cuts at: 0 72 318 1386
Size: 72 246 1068
Fragments arranged by size:
1068 246 72
EcoRI G'AATT_C
Cuts at: 0 61 232 1386
Size: 61 171 1154
Fragments arranged by size:
1154 171 61
HaeII r_GCGC'y
Cuts at: 0 1359 1386
Size: 1359 27
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 130 162 421 453 990 1022 1386
Size: 130 32 259 32 537 32 364
Fragments arranged by size:
537 364 259 130 32 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 941 1386
Size: 941 445
MscI TGG'CCA
Cuts at: 0 859 1386
Size: 859 527
MslI CAynn'nnrTG
Cuts at: 0 794 922 1024 1324 1386
Size: 794 128 102 300 62
Fragments arranged by size:
794 300 128 102 62
MspA1I CmG'CkG
Cuts at: 0 297 405 508 595 1386
Size: 297 108 103 87 791
Fragments arranged by size:
791 297 108 103 87
NspI r_CATG'y
Cuts at: 0 122 424 1386
Size: 122 302 962
Fragments arranged by size:
962 302 122
PasI CC'CwG_GG
Cuts at: 0 82 1386
Size: 82 1304
PciI A'CATG_T
Cuts at: 0 118 1386
Size: 118 1268
PflMI CCAn_nnn'nTGG
Cuts at: 0 522 1386
Size: 522 864
PshAI GACnn'nnGTC
Cuts at: 0 398 1386
Size: 398 988
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1216 1248 1386
Size: 1216 32 138
Fragments arranged by size:
1216 138 32
PstI C_TGCA'G
Cuts at: 0 59 971 1386
Size: 59 912 415
Fragments arranged by size:
912 415 59
PvuII CAG'CTG
Cuts at: 0 297 405 508 1386
Size: 297 108 103 878
Fragments arranged by size:
878 297 108 103
SacI G_AGCT'C
Cuts at: 0 143 1386
Size: 143 1243
ScaI AGT'ACT
Cuts at: 0 561 1386
Size: 561 825
SexAI A'CCwGG_T
Cuts at: 0 686 1386
Size: 686 700
SfcI C'TryA_G
Cuts at: 0 55 209 967 1386
Size: 55 154 758 419
Fragments arranged by size:
758 419 154 55
SmaI CCC'GGG
Cuts at: 0 427 1042 1386
Size: 427 615 344
Fragments arranged by size:
615 427 344
SphI G_CATG'C
Cuts at: 0 424 1386
Size: 424 962
StuI AGG'CCT
Cuts at: 0 382 1386
Size: 382 1004
StyI C'CwwG_G
Cuts at: 0 149 1386
Size: 149 1237
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 110 153 358 1133 1386
Size: 110 43 205 775 253
Fragments arranged by size:
775 253 205 110 43
TatI w'GTAC_w
Cuts at: 0 33 256 559 1386
Size: 33 223 303 827
Fragments arranged by size:
827 303 223 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 284 316 1386
Size: 284 32 1070
Fragments arranged by size:
1070 284 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1023 1317 1386
Size: 1023 294 69
Fragments arranged by size:
1023 294 69
XmaI C'CCGG_G
Cuts at: 0 425 1040 1386
Size: 425 615 346
Fragments arranged by size:
615 425 346
XmnI GAAnn'nnTTC
Cuts at: 0 232 1386
Size: 232 1154
ZraI GAC'GTC
Cuts at: 0 1350 1386
Size: 1350 36
Enzymes that do cut:
AarI AatII AcuI AfeI AflIII AlfI AlwNI ApoI
AvaI BanI BanII BbsI BbvCI BlpI Bme1580I BmrI
BplI BpmI Bpu10I BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgZI
DraIII EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI HaeII Hin4I MmeI MscI MslI MspA1I
NspI PasI PciI PflMI PshAI PsrI PstI PvuII
SacI ScaI SexAI SfcI SmaI SphI StuI StyI
TaqII TatI TstI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AflII AgeI AhdI AleI AloI
ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BbeI BcgI BciVI BclI BfrBI BglI BglII BmgBI
BmtI BpuEI BsaI BsaAI BsaBI BsiWI BsmI BsmBI
BsrFI BsrGI BssHII BstBI BstZ17I BtgI BtsI ClaI
CspCI DraI DrdI EcoRV FalI FseI FspI FspAI
HincII HindIII HpaI KasI KpnI MfeI MluI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PfoI PmeI PmlI PpiI PpuMI PsiI PspOMI
PspXI PvuI RsrII SacII SalI SanDI SapI SbfI
SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SrfI
SspI SwaI Tth111I XbaI XhoI