(Linear) (Six-base) MAPSORT of: KIEE3632.seq  Check: 1848  from: 1  to: 942



KIEE3632.orf

 With 182 enzymes: SgfI * 

                        July 3, 2012 18:13  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     264     942 
   Size:        264     678
 
ApoI r'AATT_y

Cuts at:      0     561     942 
   Size:        561     381
 
BanII G_rGCy'C

Cuts at:      0       8     555     750     942 
   Size:          8     547     195     192

  Fragments arranged by size: 

                 547     195     192       8
 
Bme1580I G_kGCm'C

Cuts at:      0     695     942 
   Size:        695     247
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     549     753     942 
   Size:        549     204     189

  Fragments arranged by size: 

                 549     204     189
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     137     942 
   Size:        137     805
 
BseYI C'CCAG_C

Cuts at:      0     183     942 
   Size:        183     759
 
BsiHKAI G_wGCw'C

Cuts at:      0     236     942 
   Size:        236     706
 
Bsp1286I G_dGCh'C

Cuts at:      0       8     236     555     695     750     942 
   Size:          8     228     319     140      55     192

  Fragments arranged by size: 

                 319     228     192     140      55       8
 
BspHI T'CATG_A

Cuts at:      0     357     893     942 
   Size:        357     536      49

  Fragments arranged by size: 

                 536     357      49
 
BtgI C'CryG_G

Cuts at:      0      47     616     942 
   Size:         47     569     326

  Fragments arranged by size: 

                 569     326      47
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      78     942 
   Size:         78     864
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     264     942 
   Size:        264     678
 
EcoRI G'AATT_C

Cuts at:      0     561     942 
   Size:        561     381
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     117     149     942 
   Size:        117      32     793

  Fragments arranged by size: 

                 793     117      32
 
HincII GTy'rAC

Cuts at:      0     602     942 
   Size:        602     340
 
HpaI GTT'AAC

Cuts at:      0     602     942 
   Size:        602     340
 
MspA1I CmG'CkG

Cuts at:      0      80     942 
   Size:         80     862
 
NcoI C'CATG_G

Cuts at:      0     616     942 
   Size:        616     326
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     336     832     942 
   Size:        336     496     110

  Fragments arranged by size: 

                 496     336     110
 
PfoI T'CCnGG_A

Cuts at:      0       8     942 
   Size:          8     934
 
SexAI A'CCwGG_T

Cuts at:      0      85     942 
   Size:         85     857
 
StyI C'CwwG_G

Cuts at:      0     136     616     942 
   Size:        136     480     326

  Fragments arranged by size: 

                 480     326     136
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     221     942 
   Size:        221     721
 
TatI w'GTAC_w

Cuts at:      0     584     942 
   Size:        584     358
 
Tth111I GACn'n_nGTC

Cuts at:      0      87     942 
   Size:         87     855
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     685     942 
   Size:        685     257
 
XmnI GAAnn'nnTTC

Cuts at:      0     215     942 
   Size:        215     727
 

 Enzymes that do cut:

     AcuI     ApoI    BanII Bme1580I     BmrI     BsaI    BseYI  BsiHKAI
 Bsp1286I    BspHI     BtgI    BtgZI  Eco57MI    EcoRI    Hin4I   HincII
     HpaI   MspA1I     NcoI    PflMI     PfoI    SexAI     StyI    TaqII
     TatI  Tth111I     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI    BmgBI     BmtI     BplI     BpmI   Bpu10I    BpuEI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI     BsgI    BsiEI
    BsiWI     BsmI    BsmBI    BspEI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI
  BstZ17I   Bsu36I     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI    EcoNI EcoO109I    EcoRV
     FalI     FseI     FspI    FspAI    HaeII  HindIII     KasI     KpnI
     MfeI     MluI     MmeI     MscI     MslI     NaeI     NarI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI     TstI     XbaI     XhoI     XmaI     ZraI