Restriction map for
pFN21AE3629
(Linear) (Six-base) MAPSORT of: KIEE3629.seq Check: 129 from: 1 to: 2466
KIEE3629.orf
With 182 enzymes: SgfI *
December 27, 2011 16:36 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 497 1759 2071 2466
Size: 497 1262 312 395
Fragments arranged by size:
1262 497 395 312
AflIII A'CryG_T
Cuts at: 0 1548 2466
Size: 1548 918
AhdI GACnn_n'nnGTC
Cuts at: 0 178 2052 2466
Size: 178 1874 414
Fragments arranged by size:
1874 414 178
AlwNI CAG_nnn'CTG
Cuts at: 0 134 1782 2466
Size: 134 1648 684
Fragments arranged by size:
1648 684 134
ApaI G_GGCC'C
Cuts at: 0 2281 2466
Size: 2281 185
ApoI r'AATT_y
Cuts at: 0 75 86 281 427 1366 1703 1737 2466
Size: 75 11 195 146 939 337 34 729
Fragments arranged by size:
939 729 337 195 146 75 34 11
AseI AT'TA_AT
Cuts at: 0 586 2417 2466
Size: 586 1831 49
Fragments arranged by size:
1831 586 49
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 911 944 1546 1579 2466
Size: 911 33 602 33 887
Fragments arranged by size:
911 887 602 33 33
BanI G'GyrC_C
Cuts at: 0 1974 2466
Size: 1974 492
BanII G_rGCy'C
Cuts at: 0 2108 2281 2466
Size: 2108 173 185
Fragments arranged by size:
2108 185 173
BbsI GAAGACnn'nnnn_
Cuts at: 0 218 1566 2201 2466
Size: 218 1348 635 265
Fragments arranged by size:
1348 635 265 218
BciVI GTATCCnnnnn_n'
Cuts at: 0 254 1080 1713 2466
Size: 254 826 633 753
Fragments arranged by size:
826 753 633 254
BclI T'GATC_A
Cuts at: 0 297 2466
Size: 297 2169
BfrBI ATG'CAT
Cuts at: 0 1489 2466
Size: 1489 977
BglII A'GATC_T
Cuts at: 0 528 745 1644 2016 2466
Size: 528 217 899 372 450
Fragments arranged by size:
899 528 450 372 217
Bme1580I G_kGCm'C
Cuts at: 0 2281 2466
Size: 2281 185
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 803 2281 2466
Size: 803 1478 185
Fragments arranged by size:
1478 803 185
BsaI GGTCTCn'nnnn_
Cuts at: 0 356 2466
Size: 356 2110
BsaWI w'CCGG_w
Cuts at: 0 2183 2466
Size: 2183 283
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2150 2180 2466
Size: 2150 30 286
Fragments arranged by size:
2150 286 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1181 2431 2447 2466
Size: 1181 1250 16 19
Fragments arranged by size:
1250 1181 19 16
BseYI C'CCAG_C
Cuts at: 0 2134 2466
Size: 2134 332
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 828 2466
Size: 828 1638
BsiHKAI G_wGCw'C
Cuts at: 0 1446 2108 2466
Size: 1446 662 358
Fragments arranged by size:
1446 662 358
Bsp1286I G_dGCh'C
Cuts at: 0 1446 2108 2281 2466
Size: 1446 662 173 185
Fragments arranged by size:
1446 662 185 173
BspEI T'CCGG_A
Cuts at: 0 2183 2466
Size: 2183 283
BspHI T'CATG_A
Cuts at: 0 1596 2466
Size: 1596 870
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 651 2466
Size: 651 1815
BsrDI GCAATG_nn'
Cuts at: 0 60 319 2466
Size: 60 259 2147
Fragments arranged by size:
2147 259 60
BsrFI r'CCGG_y
Cuts at: 0 1790 2466
Size: 1790 676
BstYI r'GATC_y
Cuts at: 0 528 745 1644 2001 2016 2466
Size: 528 217 899 357 15 450
Fragments arranged by size:
899 528 450 357 217 15
Bsu36I CC'TnA_GG
Cuts at: 0 263 692 2466
Size: 263 429 1774
Fragments arranged by size:
1774 429 263
BtgI C'CryG_G
Cuts at: 0 2068 2466
Size: 2068 398
BtsI GCAGTG_nn'
Cuts at: 0 1962 2252 2285 2466
Size: 1962 290 33 181
Fragments arranged by size:
1962 290 181 33
DraIII CAC_nnn'GTG
Cuts at: 0 1441 2373 2466
Size: 1441 932 93
Fragments arranged by size:
1441 932 93
DrdI GACnn_nn'nnGTC
Cuts at: 0 2019 2466
Size: 2019 447
EarI CTCTTCn'nnn_
Cuts at: 0 473 1123 1226 1834 2466
Size: 473 650 103 608 632
Fragments arranged by size:
650 632 608 473 103
EcoICRI GAG'CTC
Cuts at: 0 2106 2466
Size: 2106 360
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 497 803 1759 2071 2281 2466
Size: 497 306 956 312 210 185
Fragments arranged by size:
956 497 312 306 210 185
EcoO109I rG'GnC_Cy
Cuts at: 0 2085 2277 2278 2466
Size: 2085 192 1 188
Fragments arranged by size:
2085 192 188 1
EcoRI G'AATT_C
Cuts at: 0 1737 2466
Size: 1737 729
EcoRV GAT'ATC
Cuts at: 0 699 2466
Size: 699 1767
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 235 1928 2284 2466
Size: 235 1693 356 182
Fragments arranged by size:
1693 356 235 182
HaeII r_GCGC'y
Cuts at: 0 99 2466
Size: 99 2367
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 50 82 164 196 334 366 889 921
Size: 50 32 82 32 138 32 523 32
Cuts at: 921 994 1026 1602 1634 1862 1894 2018 2038
Size: 73 32 576 32 228 32 124 20
Cuts at: 2038 2050 2070 2370 2402 2466
Size: 12 20 300 32 64
Fragments arranged by size:
576 523 300 228 138 124 82 73
64 50 32 32 32 32 32 32
32 32 20 20 12
HincII GTy'rAC
Cuts at: 0 810 1182 2178 2466
Size: 810 372 996 288
Fragments arranged by size:
996 810 372 288
HindIII A'AGCT_T
Cuts at: 0 879 2466
Size: 879 1587
MfeI C'AATT_G
Cuts at: 0 1262 2466
Size: 1262 1204
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1873 2466
Size: 1873 593
MslI CAynn'nnrTG
Cuts at: 0 1622 2290 2466
Size: 1622 668 176
Fragments arranged by size:
1622 668 176
MspA1I CmG'CkG
Cuts at: 0 1054 1782 2353 2466
Size: 1054 728 571 113
Fragments arranged by size:
1054 728 571 113
NsiI A_TGCA'T
Cuts at: 0 1491 2466
Size: 1491 975
NspI r_CATG'y
Cuts at: 0 306 1493 1552 1927 2338 2466
Size: 306 1187 59 375 411 128
Fragments arranged by size:
1187 411 375 306 128 59
PasI CC'CwG_GG
Cuts at: 0 2274 2466
Size: 2274 192
PciI A'CATG_T
Cuts at: 0 1548 2466
Size: 1548 918
PflMI CCAn_nnn'nTGG
Cuts at: 0 144 1661 2466
Size: 144 1517 805
Fragments arranged by size:
1517 805 144
PpuMI rG'GwC_Cy
Cuts at: 0 2085 2466
Size: 2085 381
PsiI TTA'TAA
Cuts at: 0 502 2466
Size: 502 1964
PspOMI G'GGCC_C
Cuts at: 0 2277 2466
Size: 2277 189
PstI C_TGCA'G
Cuts at: 0 1053 2259 2466
Size: 1053 1206 207
Fragments arranged by size:
1206 1053 207
PvuII CAG'CTG
Cuts at: 0 1054 1782 2353 2466
Size: 1054 728 571 113
Fragments arranged by size:
1054 728 571 113
SacI G_AGCT'C
Cuts at: 0 2108 2466
Size: 2108 358
SapI GCTCTTCn'nnn_
Cuts at: 0 473 1226 2466
Size: 473 753 1240
Fragments arranged by size:
1240 753 473
SfcI C'TryA_G
Cuts at: 0 1049 1286 2255 2466
Size: 1049 237 969 211
Fragments arranged by size:
1049 969 237 211
SpeI A'CTAG_T
Cuts at: 0 174 2466
Size: 174 2292
SphI G_CATG'C
Cuts at: 0 1927 2466
Size: 1927 539
StuI AGG'CCT
Cuts at: 0 2438 2466
Size: 2438 28
StyI C'CwwG_G
Cuts at: 0 1478 1660 2466
Size: 1478 182 806
Fragments arranged by size:
1478 806 182
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 136 2148 2466
Size: 136 2012 318
Fragments arranged by size:
2012 318 136
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1266 1298 2466
Size: 1266 32 1168
Fragments arranged by size:
1266 1168 32
Tth111I GACn'n_nGTC
Cuts at: 0 890 933 2466
Size: 890 43 1533
Fragments arranged by size:
1533 890 43
XbaI T'CTAG_A
Cuts at: 0 2403 2466
Size: 2403 63
XmnI GAAnn'nnTTC
Cuts at: 0 821 2466
Size: 821 1645
Enzymes that do cut:
AcuI AflIII AhdI AlwNI ApaI ApoI AseI BaeI
BanI BanII BbsI BciVI BclI BfrBI BglII Bme1580I
BpmI BsaI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrDI BsrFI BstYI Bsu36I
BtgI BtsI DraIII DrdI EarI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI HaeII Hin4I HincII HindIII MfeI
MmeI MslI MspA1I NsiI NspI PasI PciI PflMI
PpuMI PsiI PspOMI PstI PvuII SacI SapI SfcI
SpeI SphI StuI StyI TaqII TstI Tth111I XbaI
XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AleI AlfI AloI ApaLI AscI AsiSI AvaI AvrII
BamHI BbeI BbvCI BcgI BglI BlpI BmgBI BmrI
BmtI BplI Bpu10I BpuEI BsaAI BsaBI BsaHI BsiEI
BsiWI BsmI BsmBI BsrBI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstZ17I BtgZI ClaI CspCI DraI
EaeI EagI EciI EcoNI FseI FspI FspAI HpaI
KasI KpnI MluI MscI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI PacI PfoI PmeI PmlI
PpiI PshAI PspXI PsrI PvuI RsrII SacII SalI
SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SrfI SspI SwaI TatI XcmI
XhoI XmaI ZraI