(Linear) (Six-base) MAPSORT of: KIEE3628.seq  Check: 4278  from: 1  to: 540



KIEE3628.orf

 With 182 enzymes: SgfI * 

                        December 27, 2011 16:36  ..

AclI AA'CG_TT

Cuts at:      0      60     540 
   Size:         60     480
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     317     540 
   Size:        317     223
 
ApoI r'AATT_y

Cuts at:      0     280     352     540 
   Size:        280      72     188

  Fragments arranged by size: 

                 280     188      72
 
BclI T'GATC_A

Cuts at:      0     132     540 
   Size:        132     408
 
Bme1580I G_kGCm'C

Cuts at:      0     169     213     540 
   Size:        169      44     327

  Fragments arranged by size: 

                 327     169      44
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     186     540 
   Size:        186     354
 
BsaBI GATnn'nnATC

Cuts at:      0     131     540 
   Size:        131     409
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      94     540 
   Size:         94     446
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     244     540 
   Size:        244     296
 
Bsp1286I G_dGCh'C

Cuts at:      0     169     213     540 
   Size:        169      44     327

  Fragments arranged by size: 

                 327     169      44
 
BsrDI GCAATG_nn'

Cuts at:      0     260     356     540 
   Size:        260      96     184

  Fragments arranged by size: 

                 260     184      96
 
BtsI GCAGTG_nn'

Cuts at:      0     232     540 
   Size:        232     308
 
DraIII CAC_nnn'GTG

Cuts at:      0     124     540 
   Size:        124     416
 
EaeI y'GGCC_r

Cuts at:      0     426     540 
   Size:        426     114
 
EarI CTCTTCn'nnn_

Cuts at:      0     395     540 
   Size:        395     145
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     186     317     540 
   Size:        186     131     223

  Fragments arranged by size: 

                 223     186     131
 
EcoO109I rG'GnC_Cy

Cuts at:      0     302     540 
   Size:        302     238
 
EcoRI G'AATT_C

Cuts at:      0     352     540 
   Size:        352     188
 
HaeII r_GCGC'y

Cuts at:      0     156     540 
   Size:        156     384
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      70     102     540 
   Size:         70      32     438

  Fragments arranged by size: 

                 438      70      32
 
MslI CAynn'nnrTG

Cuts at:      0     440     540 
   Size:        440     100
 
MspA1I CmG'CkG

Cuts at:      0      53     540 
   Size:         53     487
 
NspI r_CATG'y

Cuts at:      0     295     439     540 
   Size:        295     144     101

  Fragments arranged by size: 

                 295     144     101
 
PpuMI rG'GwC_Cy

Cuts at:      0     302     540 
   Size:        302     238
 
PsiI TTA'TAA

Cuts at:      0     502     540 
   Size:        502      38
 
PvuII CAG'CTG

Cuts at:      0      53     540 
   Size:         53     487
 
SfcI C'TryA_G

Cuts at:      0     183     540 
   Size:        183     357
 
StyI C'CwwG_G

Cuts at:      0     347     540 
   Size:        347     193
 

 Enzymes that do cut:

     AclI     AcuI     ApoI     BclI Bme1580I     BpmI    BsaBI    BseRI
     BsgI Bsp1286I    BsrDI     BtsI   DraIII     EaeI     EarI  Eco57MI
 EcoO109I    EcoRI    HaeII    Hin4I     MslI   MspA1I     NspI    PpuMI
     PsiI    PvuII     SfcI     StyI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI    BfrBI     BglI    BglII
     BlpI    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaHI    BsaWI    BsaXI    BseYI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I
   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI     DrdI     EagI
     EciI  EcoICRI    EcoNI    EcoRV     FalI     FseI     FspI    FspAI
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MscI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PshAI   PspOMI    PspXI     PsrI     PstI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI