Restriction map for
pFN21AE3624
(Linear) (Six-base) MAPSORT of: KIEE3624.seq Check: 6738 from: 1 to: 1815
KIEE3624.orf
With 182 enzymes: SgfI *
December 27, 2011 16:36 ..
AccI GT'mk_AC
Cuts at: 0 587 1815
Size: 587 1228
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 43 1525 1815
Size: 43 1482 290
Fragments arranged by size:
1482 290 43
AfeI AGC'GCT
Cuts at: 0 28 1815
Size: 28 1787
AflII C'TTAA_G
Cuts at: 0 1581 1815
Size: 1581 234
AflIII A'CryG_T
Cuts at: 0 821 1270 1815
Size: 821 449 545
Fragments arranged by size:
821 545 449
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 526 1815
Size: 526 1289
AlwNI CAG_nnn'CTG
Cuts at: 0 417 795 852 1815
Size: 417 378 57 963
Fragments arranged by size:
963 417 378 57
ApaI G_GGCC'C
Cuts at: 0 740 1815
Size: 740 1075
ApoI r'AATT_y
Cuts at: 0 774 1588 1815
Size: 774 814 227
Fragments arranged by size:
814 774 227
AseI AT'TA_AT
Cuts at: 0 1363 1815
Size: 1363 452
AvaI C'yCGr_G
Cuts at: 0 287 440 756 863 1815
Size: 287 153 316 107 952
Fragments arranged by size:
952 316 287 153 107
BamHI G'GATC_C
Cuts at: 0 1526 1815
Size: 1526 289
BanI G'GyrC_C
Cuts at: 0 82 630 1815
Size: 82 548 1185
Fragments arranged by size:
1185 548 82
BanII G_rGCy'C
Cuts at: 0 536 554 740 1006 1106 1264 1815
Size: 536 18 186 266 100 158 551
Fragments arranged by size:
551 536 266 186 158 100 18
BbsI GAAGACnn'nnnn_
Cuts at: 0 796 1226 1293 1815
Size: 796 430 67 522
Fragments arranged by size:
796 522 430 67
BbvCI CC'TCA_GC
Cuts at: 0 319 364 1815
Size: 319 45 1451
Fragments arranged by size:
1451 319 45
BglI GCCn_nnn'nGGC
Cuts at: 0 115 1815
Size: 115 1700
BglII A'GATC_T
Cuts at: 0 1487 1815
Size: 1487 328
BlpI GC'TnA_GC
Cuts at: 0 1337 1815
Size: 1337 478
Bme1580I G_kGCm'C
Cuts at: 0 635 740 789 1815
Size: 635 105 49 1026
Fragments arranged by size:
1026 635 105 49
BmgBI CAC'GTC
Cuts at: 0 1271 1815
Size: 1271 544
BmrI ACTGGGnnnn_n'
Cuts at: 0 266 1815
Size: 266 1549
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 878 1815
Size: 878 937
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 435 616 732 871 1710 1731 1815
Size: 151 284 181 116 139 839 21 84
Fragments arranged by size:
839 284 181 151 139 116 84 21
Bpu10I CC'TnA_GC
Cuts at: 0 319 364 853 1073 1815
Size: 319 45 489 220 742
Fragments arranged by size:
742 489 319 220 45
BsaI GGTCTCn'nnnn_
Cuts at: 0 237 1128 1565 1815
Size: 237 891 437 250
Fragments arranged by size:
891 437 250 237
BsaAI yAC'GTr
Cuts at: 0 824 1815
Size: 824 991
BsaHI Gr'CG_yC
Cuts at: 0 1769 1815
Size: 1769 46
BsaWI w'CCGG_w
Cuts at: 0 458 476 1648 1815
Size: 458 18 1172 167
Fragments arranged by size:
1172 458 167 18
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 3 195 1815
Size: 3 192 1620
Fragments arranged by size:
1620 192 3
BseYI C'CCAG_C
Cuts at: 0 5 739 1815
Size: 5 734 1076
Fragments arranged by size:
1076 734 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 522 895 1815
Size: 522 373 920
Fragments arranged by size:
920 522 373
BsiHKAI G_wGCw'C
Cuts at: 0 536 1264 1499 1815
Size: 536 728 235 316
Fragments arranged by size:
728 536 316 235
BsmBI CGTCTCn'nnnn_
Cuts at: 0 706 758 1815
Size: 706 52 1057
Fragments arranged by size:
1057 706 52
Bsp1286I G_dGCh'C
Cuts at: 0 536 554 635 740 789 1006 1106 1264
Size: 536 18 81 105 49 217 100 158
Cuts at: 1264 1499 1815
Size: 235 316
Fragments arranged by size:
536 316 235 217 158 105 100 81
49 18
BspEI T'CCGG_A
Cuts at: 0 458 1648 1815
Size: 458 1190 167
Fragments arranged by size:
1190 458 167
BspHI T'CATG_A
Cuts at: 0 830 1815
Size: 830 985
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 380 917 1784 1815
Size: 380 537 867 31
Fragments arranged by size:
867 537 380 31
BsrBI CCG'CTC
Cuts at: 0 300 746 1815
Size: 300 446 1069
Fragments arranged by size:
1069 446 300
BsrDI GCAATG_nn'
Cuts at: 0 930 1331 1815
Size: 930 401 484
Fragments arranged by size:
930 484 401
BsrFI r'CCGG_y
Cuts at: 0 156 1815
Size: 156 1659
BsrGI T'GTAC_A
Cuts at: 0 1273 1815
Size: 1273 542
BssSI C'ACGA_G
Cuts at: 0 653 940 1815
Size: 653 287 875
Fragments arranged by size:
875 653 287
BstBI TT'CG_AA
Cuts at: 0 807 1815
Size: 807 1008
BstEII G'GTnAC_C
Cuts at: 0 399 1815
Size: 399 1416
BstXI CCAn_nnnn'nTGG
Cuts at: 0 495 996 1815
Size: 495 501 819
Fragments arranged by size:
819 501 495
BstYI r'GATC_y
Cuts at: 0 1025 1236 1487 1526 1815
Size: 1025 211 251 39 289
Fragments arranged by size:
1025 289 251 211 39
Bsu36I CC'TnA_GG
Cuts at: 0 1352 1815
Size: 1352 463
BtgI C'CryG_G
Cuts at: 0 915 1658 1815
Size: 915 743 157
Fragments arranged by size:
915 743 157
BtsI GCAGTG_nn'
Cuts at: 0 318 624 853 1324 1815
Size: 318 306 229 471 491
Fragments arranged by size:
491 471 318 306 229
DraIII CAC_nnn'GTG
Cuts at: 0 707 1815
Size: 707 1108
DrdI GACnn_nn'nnGTC
Cuts at: 0 1729 1815
Size: 1729 86
EaeI y'GGCC_r
Cuts at: 0 693 1665 1793 1815
Size: 693 972 128 22
Fragments arranged by size:
972 693 128 22
EarI CTCTTCn'nnn_
Cuts at: 0 756 941 1387 1786 1815
Size: 756 185 446 399 29
Fragments arranged by size:
756 446 399 185 29
EcoICRI GAG'CTC
Cuts at: 0 534 1262 1815
Size: 534 728 553
Fragments arranged by size:
728 553 534
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 43 151 435 616 732 871 1525 1710
Size: 43 108 284 181 116 139 654 185
Cuts at: 1710 1731 1815
Size: 21 84
Fragments arranged by size:
654 284 185 181 139 116 108 84
43 21
EcoO109I rG'GnC_Cy
Cuts at: 0 642 729 906 1317 1815
Size: 642 87 177 411 498
Fragments arranged by size:
642 498 411 177 87
EcoRI G'AATT_C
Cuts at: 0 774 1815
Size: 774 1041
FspI TGC'GCA
Cuts at: 0 1759 1815
Size: 1759 56
FspAI rTGC'GCAy
Cuts at: 0 1759 1815
Size: 1759 56
HaeII r_GCGC'y
Cuts at: 0 30 1815
Size: 30 1785
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 236 268 412 444 1191 1221 1223 1253
Size: 236 32 144 32 747 30 2 30
Cuts at: 1253 1815
Size: 562
Fragments arranged by size:
747 562 236 144 32 32 30 30
2
HincII GTy'rAC
Cuts at: 0 588 1815
Size: 588 1227
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1169 1815
Size: 1169 646
MscI TGG'CCA
Cuts at: 0 1667 1795 1815
Size: 1667 128 20
Fragments arranged by size:
1667 128 20
MslI CAynn'nnrTG
Cuts at: 0 1738 1815
Size: 1738 77
MspA1I CmG'CkG
Cuts at: 0 323 332 569 1815
Size: 323 9 237 1246
Fragments arranged by size:
1246 323 237 9
NcoI C'CATG_G
Cuts at: 0 1658 1815
Size: 1658 157
NdeI CA'TA_TG
Cuts at: 0 973 1815
Size: 973 842
NspI r_CATG'y
Cuts at: 0 903 1427 1815
Size: 903 524 388
Fragments arranged by size:
903 524 388
PasI CC'CwG_GG
Cuts at: 0 733 789 1815
Size: 733 56 1026
Fragments arranged by size:
1026 733 56
PflMI CCAn_nnn'nTGG
Cuts at: 0 417 795 1815
Size: 417 378 1020
Fragments arranged by size:
1020 417 378
PfoI T'CCnGG_A
Cuts at: 0 212 1522 1815
Size: 212 1310 293
Fragments arranged by size:
1310 293 212
PmlI CAC'GTG
Cuts at: 0 824 1815
Size: 824 991
PpuMI rG'GwC_Cy
Cuts at: 0 906 1317 1815
Size: 906 411 498
Fragments arranged by size:
906 498 411
PshAI GACnn'nnGTC
Cuts at: 0 1140 1815
Size: 1140 675
PspOMI G'GGCC_C
Cuts at: 0 736 1815
Size: 736 1079
PstI C_TGCA'G
Cuts at: 0 548 848 1815
Size: 548 300 967
Fragments arranged by size:
967 548 300
PvuII CAG'CTG
Cuts at: 0 569 1815
Size: 569 1246
SacI G_AGCT'C
Cuts at: 0 536 1264 1815
Size: 536 728 551
Fragments arranged by size:
728 551 536
SalI G'TCGA_C
Cuts at: 0 586 1815
Size: 586 1229
SapI GCTCTTCn'nnn_
Cuts at: 0 1387 1786 1815
Size: 1387 399 29
Fragments arranged by size:
1387 399 29
SbfI CC_TGCA'GG
Cuts at: 0 548 1815
Size: 548 1267
SexAI A'CCwGG_T
Cuts at: 0 581 1815
Size: 581 1234
SfcI C'TryA_G
Cuts at: 0 544 844 1815
Size: 544 300 971
Fragments arranged by size:
971 544 300
SmaI CCC'GGG
Cuts at: 0 289 442 758 1815
Size: 289 153 316 1057
Fragments arranged by size:
1057 316 289 153
SmlI C'TyrA_G
Cuts at: 0 1581 1815
Size: 1581 234
StyI C'CwwG_G
Cuts at: 0 813 1658 1815
Size: 813 845 157
Fragments arranged by size:
845 813 157
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1651 1815
Size: 1651 164
TatI w'GTAC_w
Cuts at: 0 1273 1815
Size: 1273 542
XbaI T'CTAG_A
Cuts at: 0 1209 1815
Size: 1209 606
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 796 1815
Size: 796 1019
XmaI C'CCGG_G
Cuts at: 0 287 440 756 1815
Size: 287 153 316 1059
Fragments arranged by size:
1059 316 287 153
XmnI GAAnn'nnTTC
Cuts at: 0 1510 1815
Size: 1510 305
Enzymes that do cut:
AccI AcuI AfeI AflII AflIII AlfI AlwNI ApaI
ApoI AseI AvaI BamHI BanI BanII BbsI BbvCI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BsaI BsaAI BsaHI BsaWI BseRI BseYI BsgI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtsI DraIII DrdI EaeI EarI EcoICRI Eco57MI
EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII MmeI
MscI MslI MspA1I NcoI NdeI NspI PasI PflMI
PfoI PmlI PpuMI PshAI PspOMI PstI PvuII SacI
SalI SapI SbfI SexAI SfcI SmaI SmlI StyI
TaqII TatI XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AgeI AhdI AleI AloI
ApaLI AscI AsiSI AvrII BaeI BbeI BcgI BciVI
BclI BfrBI BmtI BpuEI BsaBI BsaXI BsiEI BsiWI
BsmI BssHII BstAPI BstZ17I BtgZI ClaI CspCI DraI
EagI EciI EcoNI EcoRV FalI FseI HindIII HpaI
KasI KpnI MfeI MluI NaeI NarI NgoMIV NheI
NotI NruI NsiI PacI PciI PmeI PpiI PsiI
PspXI PsrI PvuI RsrII SacII SanDI ScaI SfiI
SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI
StuI SwaI TstI Tth111I XhoI ZraI