Restriction map for
pFN21AE3622
(Linear) (Six-base) MAPSORT of: KIEE3622.seq Check: 7955 from: 1 to: 1125
KIEE3622.orf
With 182 enzymes: SgfI *
July 3, 2012 18:13 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 220 1125
Size: 220 905
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 766 798 1125
Size: 766 32 327
Fragments arranged by size:
766 327 32
ApaI G_GGCC'C
Cuts at: 0 1099 1125
Size: 1099 26
AvaI C'yCGr_G
Cuts at: 0 80 918 1125
Size: 80 838 207
Fragments arranged by size:
838 207 80
BanI G'GyrC_C
Cuts at: 0 107 257 486 904 960 1125
Size: 107 150 229 418 56 165
Fragments arranged by size:
418 229 165 150 107 56
BanII G_rGCy'C
Cuts at: 0 1099 1125
Size: 1099 26
BbeI G_GCGC'C
Cuts at: 0 111 964 1125
Size: 111 853 161
Fragments arranged by size:
853 161 111
BglI GCCn_nnn'nGGC
Cuts at: 0 409 1125
Size: 409 716
BglII A'GATC_T
Cuts at: 0 251 1125
Size: 251 874
Bme1580I G_kGCm'C
Cuts at: 0 491 1099 1125
Size: 491 608 26
Fragments arranged by size:
608 491 26
BmgBI CAC'GTC
Cuts at: 0 858 1125
Size: 858 267
BmrI ACTGGGnnnn_n'
Cuts at: 0 243 298 1125
Size: 243 55 827
Fragments arranged by size:
827 243 55
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 485 727 1125
Size: 485 242 398
Fragments arranged by size:
485 398 242
BsaI GGTCTCn'nnnn_
Cuts at: 0 367 820 1125
Size: 367 453 305
Fragments arranged by size:
453 367 305
BsaAI yAC'GTr
Cuts at: 0 159 399 1125
Size: 159 240 726
Fragments arranged by size:
726 240 159
BsaBI GATnn'nnATC
Cuts at: 0 982 1125
Size: 982 143
BsaHI Gr'CG_yC
Cuts at: 0 108 125 961 1125
Size: 108 17 836 164
Fragments arranged by size:
836 164 108 17
BsaWI w'CCGG_w
Cuts at: 0 764 771 1125
Size: 764 7 354
Fragments arranged by size:
764 354 7
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 22 52 458 488 631 661 766 796
Size: 22 30 406 30 143 30 105 30
Cuts at: 796 1125
Size: 329
Fragments arranged by size:
406 329 143 105 30 30 30 30
22
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 310 687 1125
Size: 310 377 438
Fragments arranged by size:
438 377 310
BseYI C'CCAG_C
Cuts at: 0 64 964 1125
Size: 64 900 161
Fragments arranged by size:
900 161 64
BsiEI CG_ry'CG
Cuts at: 0 611 1125
Size: 611 514
BsiHKAI G_wGCw'C
Cuts at: 0 302 1125
Size: 302 823
BsmBI CGTCTCn'nnnn_
Cuts at: 0 461 1125
Size: 461 664
Bsp1286I G_dGCh'C
Cuts at: 0 302 491 1099 1125
Size: 302 189 608 26
Fragments arranged by size:
608 302 189 26
BspEI T'CCGG_A
Cuts at: 0 771 1125
Size: 771 354
BspHI T'CATG_A
Cuts at: 0 566 845 1125
Size: 566 279 280
Fragments arranged by size:
566 280 279
BsrBI CCG'CTC
Cuts at: 0 615 759 1125
Size: 615 144 366
Fragments arranged by size:
615 366 144
BsrDI GCAATG_nn'
Cuts at: 0 759 923 1125
Size: 759 164 202
Fragments arranged by size:
759 202 164
BssSI C'ACGA_G
Cuts at: 0 823 1125
Size: 823 302
BstAPI GCAn_nnn'nTGC
Cuts at: 0 306 1125
Size: 306 819
BstEII G'GTnAC_C
Cuts at: 0 474 1125
Size: 474 651
BstXI CCAn_nnnn'nTGG
Cuts at: 0 399 1125
Size: 399 726
BstYI r'GATC_y
Cuts at: 0 251 572 1125
Size: 251 321 553
Fragments arranged by size:
553 321 251
BtgI C'CryG_G
Cuts at: 0 605 1125
Size: 605 520
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 972 1125
Size: 972 153
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 432 467 1125
Size: 432 35 658
Fragments arranged by size:
658 432 35
DraIII CAC_nnn'GTG
Cuts at: 0 306 486 1125
Size: 306 180 639
Fragments arranged by size:
639 306 180
EaeI y'GGCC_r
Cuts at: 0 389 401 543 608 680 732 1125
Size: 389 12 142 65 72 52 393
Fragments arranged by size:
393 389 142 72 65 52 12
EagI C'GGCC_G
Cuts at: 0 608 1125
Size: 608 517
EarI CTCTTCn'nnn_
Cuts at: 0 2 705 706 1125
Size: 2 703 1 419
Fragments arranged by size:
703 419 2 1
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 220 485 727 1125
Size: 220 265 242 398
Fragments arranged by size:
398 265 242 220
EcoO109I rG'GnC_Cy
Cuts at: 0 321 549 1096 1125
Size: 321 228 547 29
Fragments arranged by size:
547 321 228 29
HaeII r_GCGC'y
Cuts at: 0 111 781 964 1125
Size: 111 670 183 161
Fragments arranged by size:
670 183 161 111
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 22 54 458 490 1125
Size: 22 32 404 32 635
Fragments arranged by size:
635 404 32 32 22
KasI G'GCGC_C
Cuts at: 0 107 960 1125
Size: 107 853 165
Fragments arranged by size:
853 165 107
MscI TGG'CCA
Cuts at: 0 403 682 734 1125
Size: 403 279 52 391
Fragments arranged by size:
403 391 279 52
MslI CAynn'nnrTG
Cuts at: 0 397 454 910 1125
Size: 397 57 456 215
Fragments arranged by size:
456 397 215 57
NarI GG'CG_CC
Cuts at: 0 108 961 1125
Size: 108 853 164
Fragments arranged by size:
853 164 108
NspI r_CATG'y
Cuts at: 0 48 1125
Size: 48 1077
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 766 798 1125
Size: 766 32 327
Fragments arranged by size:
766 327 32
PpuMI rG'GwC_Cy
Cuts at: 0 549 1125
Size: 549 576
PspOMI G'GGCC_C
Cuts at: 0 1095 1125
Size: 1095 30
SapI GCTCTTCn'nnn_
Cuts at: 0 706 1125
Size: 706 419
ScaI AGT'ACT
Cuts at: 0 1009 1125
Size: 1009 116
SfcI C'TryA_G
Cuts at: 0 591 1125
Size: 591 534
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 409 1125
Size: 409 716
SfoI GGC'GCC
Cuts at: 0 109 962 1125
Size: 109 853 163
Fragments arranged by size:
853 163 109
SmaI CCC'GGG
Cuts at: 0 920 1125
Size: 920 205
StyI C'CwwG_G
Cuts at: 0 335 1125
Size: 335 790
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 317 331 493 571 1125
Size: 317 14 162 78 554
Fragments arranged by size:
554 317 162 78 14
TatI w'GTAC_w
Cuts at: 0 1007 1125
Size: 1007 118
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 631 663 1125
Size: 631 32 462
Fragments arranged by size:
631 462 32
Tth111I GACn'n_nGTC
Cuts at: 0 472 1125
Size: 472 653
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 181 528 1125
Size: 181 347 597
Fragments arranged by size:
597 347 181
XmaI C'CCGG_G
Cuts at: 0 918 1125
Size: 918 207
Enzymes that do cut:
AcuI AloI ApaI AvaI BanI BanII BbeI BglI
BglII Bme1580I BmgBI BmrI BpmI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BsrBI BsrDI BssSI BstAPI BstEII
BstXI BstYI BtgI BtgZI CspCI DraIII EaeI EagI
EarI Eco57MI EcoO109I HaeII Hin4I KasI MscI MslI
NarI NspI PpiI PpuMI PspOMI SapI ScaI SfcI
SfiI SfoI SmaI StyI TaqII TatI TstI Tth111I
XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbsI BbvCI BcgI
BciVI BclI BfrBI BlpI BmtI BplI Bpu10I BpuEI
BsgI BsiWI BsmI BspMI BsrFI BsrGI BssHII BstBI
BstZ17I Bsu36I BtsI ClaI DraI DrdI EciI EcoICRI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI HincII
HindIII HpaI KpnI MfeI MluI MmeI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PciI PflMI PfoI PmeI PmlI PshAI PsiI
PspXI PsrI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SbfI SexAI SgfI SgrAI SmlI SnaBI
SpeI SphI SrfI SspI StuI SwaI XbaI XhoI
XmnI ZraI