(Linear) (Six-base) MAPSORT of: KIEE3621.seq  Check: 2319  from: 1  to: 648



KIEE3621.orf

 With 182 enzymes: SgfI * 

                        December 27, 2011 16:36  ..

AflIII A'CryG_T

Cuts at:      0     563     648 
   Size:        563      85
 
BanI G'GyrC_C

Cuts at:      0       4     256     648 
   Size:          4     252     392

  Fragments arranged by size: 

                 392     252       4
 
Bme1580I G_kGCm'C

Cuts at:      0       7     648 
   Size:          7     641
 
BsiHKAI G_wGCw'C

Cuts at:      0     127     648 
   Size:        127     521
 
Bsp1286I G_dGCh'C

Cuts at:      0       7     127     648 
   Size:          7     120     521

  Fragments arranged by size: 

                 521     120       7
 
BsrDI GCAATG_nn'

Cuts at:      0     275     648 
   Size:        275     373
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     166     648 
   Size:        166     482
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     324     648 
   Size:        324     324
 
BtsI GCAGTG_nn'

Cuts at:      0     637     648 
   Size:        637      11
 
DraI TTT'AAA

Cuts at:      0      36     648 
   Size:         36     612
 
EcoRV GAT'ATC

Cuts at:      0     217     648 
   Size:        217     431
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      61     648 
   Size:         61     587
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      45     648 
   Size:         45     603
 
MslI CAynn'nnrTG

Cuts at:      0     189     361     648 
   Size:        189     172     287

  Fragments arranged by size: 

                 287     189     172
 
NdeI CA'TA_TG

Cuts at:      0     294     648 
   Size:        294     354
 
NspI r_CATG'y

Cuts at:      0     567     648 
   Size:        567      81
 
PciI A'CATG_T

Cuts at:      0     563     648 
   Size:        563      85
 
SfcI C'TryA_G

Cuts at:      0     251     404     648 
   Size:        251     153     244

  Fragments arranged by size: 

                 251     244     153
 

 Enzymes that do cut:

   AflIII     BanI Bme1580I  BsiHKAI Bsp1286I    BsrDI    BstXI    BtgZI
     BtsI     DraI    EcoRV     FalI     MmeI     MslI     NdeI     NspI
     PciI     SfcI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI    BmgBI     BmrI     BmtI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstYI  BstZ17I   Bsu36I     BtgI     ClaI    CspCI
   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
    EcoNI EcoO109I    EcoRI     FseI     FspI    FspAI    HaeII    Hin4I
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI
   MspA1I     NaeI     NarI     NcoI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PasI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI