Restriction map for
pFN21AE3620
(Linear) (Six-base) MAPSORT of: KIEE3620.seq Check: 2448 from: 1 to: 669
KIEE3620.orf
With 182 enzymes: SgfI *
December 27, 2011 16:36 ..
AatII G_ACGT'C
Cuts at: 0 452 669
Size: 452 217
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 452 484 669
Size: 452 32 185
Fragments arranged by size:
452 185 32
AlwNI CAG_nnn'CTG
Cuts at: 0 502 669
Size: 502 167
AvaI C'yCGr_G
Cuts at: 0 31 45 669
Size: 31 14 624
Fragments arranged by size:
624 31 14
AvrII C'CTAG_G
Cuts at: 0 271 669
Size: 271 398
BanI G'GyrC_C
Cuts at: 0 55 669
Size: 55 614
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 481 515 669
Size: 481 34 154
Fragments arranged by size:
481 154 34
BclI T'GATC_A
Cuts at: 0 203 326 669
Size: 203 123 343
Fragments arranged by size:
343 203 123
Bme1580I G_kGCm'C
Cuts at: 0 58 669
Size: 58 611
BmgBI CAC'GTC
Cuts at: 0 528 669
Size: 528 141
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 586 669
Size: 586 83
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 87 669
Size: 76 11 582
Fragments arranged by size:
582 76 11
BsaI GGTCTCn'nnnn_
Cuts at: 0 94 669
Size: 94 575
BsaHI Gr'CG_yC
Cuts at: 0 449 669
Size: 449 220
BsaWI w'CCGG_w
Cuts at: 0 583 669
Size: 583 86
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 578 669
Size: 578 91
BsiHKAI G_wGCw'C
Cuts at: 0 572 669
Size: 572 97
BsmBI CGTCTCn'nnnn_
Cuts at: 0 370 586 669
Size: 370 216 83
Fragments arranged by size:
370 216 83
Bsp1286I G_dGCh'C
Cuts at: 0 58 572 669
Size: 58 514 97
Fragments arranged by size:
514 97 58
BspEI T'CCGG_A
Cuts at: 0 583 669
Size: 583 86
BsrGI T'GTAC_A
Cuts at: 0 432 669
Size: 432 237
BssHII G'CGCG_C
Cuts at: 0 18 669
Size: 18 651
BstAPI GCAn_nnn'nTGC
Cuts at: 0 62 502 669
Size: 62 440 167
Fragments arranged by size:
440 167 62
BstXI CCAn_nnnn'nTGG
Cuts at: 0 85 669
Size: 85 584
BstYI r'GATC_y
Cuts at: 0 318 669
Size: 318 351
DraIII CAC_nnn'GTG
Cuts at: 0 62 669
Size: 62 607
EaeI y'GGCC_r
Cuts at: 0 108 243 669
Size: 108 135 426
Fragments arranged by size:
426 135 108
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 87 669
Size: 76 11 582
Fragments arranged by size:
582 76 11
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 372 404 669
Size: 372 32 265
Fragments arranged by size:
372 265 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 209 358 669
Size: 209 149 311
Fragments arranged by size:
311 209 149
MscI TGG'CCA
Cuts at: 0 245 669
Size: 245 424
MslI CAynn'nnrTG
Cuts at: 0 434 669
Size: 434 235
MspA1I CmG'CkG
Cuts at: 0 107 669
Size: 107 562
SspI AAT'ATT
Cuts at: 0 339 669
Size: 339 330
StyI C'CwwG_G
Cuts at: 0 271 669
Size: 271 398
TatI w'GTAC_w
Cuts at: 0 432 518 669
Size: 432 86 151
Fragments arranged by size:
432 151 86
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 565 597 669
Size: 565 32 72
Fragments arranged by size:
565 72 32
ZraI GAC'GTC
Cuts at: 0 450 669
Size: 450 219
Enzymes that do cut:
AatII AloI AlwNI AvaI AvrII BanI BcgI BclI
Bme1580I BmgBI BplI BpmI BsaI BsaHI BsaWI BseRI
BsiHKAI BsmBI Bsp1286I BspEI BsrGI BssHII BstAPI BstXI
BstYI DraIII EaeI Eco57MI Hin4I MmeI MscI MslI
MspA1I SspI StyI TatI TstI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI ApaI ApaLI ApoI AscI
AseI AsiSI BaeI BamHI BanII BbeI BbsI BbvCI
BciVI BfrBI BglI BglII BlpI BmrI BmtI Bpu10I
BpuEI BsaAI BsaBI BsaXI BseYI BsgI BsiEI BsiWI
BsmI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstBI
BstEII BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EagI EarI EciI EcoICRI EcoNI EcoO109I
EcoRI EcoRV FalI FseI FspI FspAI HaeII HincII
HindIII HpaI KasI KpnI MfeI MluI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI StuI SwaI TaqII
Tth111I XbaI XcmI XhoI XmaI XmnI