(Linear) (Six-base) MAPSORT of: KIEE3619.seq  Check: 6223  from: 1  to: 582



KIEE3619.orf

 With 182 enzymes: SgfI * 

                        December 27, 2011 16:36  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     450     582 
   Size:        450     132
 
AfeI AGC'GCT

Cuts at:      0     478     582 
   Size:        478     104
 
AleI CACnn'nnGTG

Cuts at:      0     331     582 
   Size:        331     251
 
AlwNI CAG_nnn'CTG

Cuts at:      0      12     237     582 
   Size:         12     225     345

  Fragments arranged by size: 

                 345     225      12
 
AvrII C'CTAG_G

Cuts at:      0     570     582 
   Size:        570      12
 
BanII G_rGCy'C

Cuts at:      0     500     582 
   Size:        500      82
 
BclI T'GATC_A

Cuts at:      0      74     582 
   Size:         74     508
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     495     582 
   Size:        495      87
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      45     582 
   Size:         45     537
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      40     130     327     582 
   Size:         40      90     197     255

  Fragments arranged by size: 

                 255     197      90      40
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     502     582 
   Size:        502      80
 
BsiHKAI G_wGCw'C

Cuts at:      0     345     582 
   Size:        345     237
 
Bsp1286I G_dGCh'C

Cuts at:      0     345     500     582 
   Size:        345     155      82

  Fragments arranged by size: 

                 345     155      82
 
Bsu36I CC'TnA_GG

Cuts at:      0      47     582 
   Size:         47     535
 
BtgI C'CryG_G

Cuts at:      0     369     408     582 
   Size:        369      39     174

  Fragments arranged by size: 

                 369     174      39
 
EaeI y'GGCC_r

Cuts at:      0     251     582 
   Size:        251     331
 
EarI CTCTTCn'nnn_

Cuts at:      0     488     582 
   Size:        488      94
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      40     130     327     450     582 
   Size:         40      90     197     123     132

  Fragments arranged by size: 

                 197     132     123      90      40
 
EcoO109I rG'GnC_Cy

Cuts at:      0     322     582 
   Size:        322     260
 
HaeII r_GCGC'y

Cuts at:      0     480     582 
   Size:        480     102
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      26     260     292     582 
   Size:         26     234      32     290

  Fragments arranged by size: 

                 290     234      32      26
 
MscI TGG'CCA

Cuts at:      0     253     582 
   Size:        253     329
 
MslI CAynn'nnrTG

Cuts at:      0     290     331     582 
   Size:        290      41     251

  Fragments arranged by size: 

                 290     251      41
 
MspA1I CmG'CkG

Cuts at:      0     257     582 
   Size:        257     325
 
NcoI C'CATG_G

Cuts at:      0     408     582 
   Size:        408     174
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      12     582 
   Size:         12     570
 
PpuMI rG'GwC_Cy

Cuts at:      0     322     582 
   Size:        322     260
 
SanDI GG'GwC_CC

Cuts at:      0     322     582 
   Size:        322     260
 
SapI GCTCTTCn'nnn_

Cuts at:      0     488     582 
   Size:        488      94
 
SfcI C'TryA_G

Cuts at:      0     525     582 
   Size:        525      57
 
StuI AGG'CCT

Cuts at:      0     569     582 
   Size:        569      13
 
StyI C'CwwG_G

Cuts at:      0     408     570     582 
   Size:        408     162      12

  Fragments arranged by size: 

                 408     162      12
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     421     582 
   Size:        421     161
 
Tth111I GACn'n_nGTC

Cuts at:      0      88     582 
   Size:         88     494
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     508     553     582 
   Size:        508      45      29

  Fragments arranged by size: 

                 508      45      29
 

 Enzymes that do cut:

     AcuI     AfeI     AleI    AlwNI    AvrII    BanII     BclI     BmrI
     BplI     BpmI    BseRI  BsiHKAI Bsp1286I   Bsu36I     BtgI     EaeI
     EarI  Eco57MI EcoO109I    HaeII    Hin4I     MscI     MslI   MspA1I
     NcoI    PflMI    PpuMI    SanDI     SapI     SfcI     StuI     StyI
    TaqII  Tth111I     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI    AflII   AflIII     AgeI
     AhdI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI     AseI
    AsiSI     AvaI     BaeI    BamHI     BanI     BbeI     BbsI    BbvCI
     BcgI    BciVI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmtI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseYI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EciI  EcoICRI
    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     NaeI
     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI     PfoI     PmeI     PmlI     PpiI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     SwaI     TatI     TstI     XbaI     XhoI     XmaI     XmnI
     ZraI