Restriction map for
pFN21AE3618
(Linear) (Six-base) MAPSORT of: KIEE3618.seq Check: 7862 from: 1 to: 894
KIEE3618.orf
With 182 enzymes: SgfI *
December 27, 2011 16:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 139 894
Size: 139 755
Acc65I G'GTAC_C
Cuts at: 0 590 894
Size: 590 304
AfeI AGC'GCT
Cuts at: 0 85 894
Size: 85 809
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 892 894
Size: 892 2
AlwNI CAG_nnn'CTG
Cuts at: 0 140 165 459 519 562 894
Size: 140 25 294 60 43 332
Fragments arranged by size:
332 294 140 60 43 25
AvaI C'yCGr_G
Cuts at: 0 313 894
Size: 313 581
AvrII C'CTAG_G
Cuts at: 0 117 894
Size: 117 777
BanI G'GyrC_C
Cuts at: 0 208 306 590 894
Size: 208 98 284 304
Fragments arranged by size:
304 284 208 98
BanII G_rGCy'C
Cuts at: 0 256 526 698 861 894
Size: 256 270 172 163 33
Fragments arranged by size:
270 256 172 163 33
BbeI G_GCGC'C
Cuts at: 0 212 894
Size: 212 682
BbsI GAAGACnn'nnnn_
Cuts at: 0 557 894
Size: 557 337
BbvCI CC'TCA_GC
Cuts at: 0 302 894
Size: 302 592
BglI GCCn_nnn'nGGC
Cuts at: 0 565 676 894
Size: 565 111 218
Fragments arranged by size:
565 218 111
Bme1580I G_kGCm'C
Cuts at: 0 639 828 847 894
Size: 639 189 19 47
Fragments arranged by size:
639 189 47 19
BmgBI CAC'GTC
Cuts at: 0 752 894
Size: 752 142
BmrI ACTGGGnnnn_n'
Cuts at: 0 63 894
Size: 63 831
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 525 894
Size: 525 369
Bpu10I CC'TnA_GC
Cuts at: 0 302 596 894
Size: 302 294 298
Fragments arranged by size:
302 298 294
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 712 894
Size: 712 182
BsaAI yAC'GTr
Cuts at: 0 150 894
Size: 150 744
BsaHI Gr'CG_yC
Cuts at: 0 209 894
Size: 209 685
BsaWI w'CCGG_w
Cuts at: 0 464 546 894
Size: 464 82 348
Fragments arranged by size:
464 348 82
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 736 766 894
Size: 736 30 128
Fragments arranged by size:
736 128 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 205 214 217 274 757 894
Size: 205 9 3 57 483 137
Fragments arranged by size:
483 205 137 57 9 3
BseYI C'CCAG_C
Cuts at: 0 445 512 519 894
Size: 445 67 7 375
Fragments arranged by size:
445 375 67 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 13 517 894
Size: 13 504 377
Fragments arranged by size:
504 377 13
Bsp1286I G_dGCh'C
Cuts at: 0 256 526 639 698 828 847 861 894
Size: 256 270 113 59 130 19 14 33
Fragments arranged by size:
270 256 130 113 59 33 19 14
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 139 894
Size: 139 755
BsrBI CCG'CTC
Cuts at: 0 98 894
Size: 98 796
BsrFI r'CCGG_y
Cuts at: 0 61 331 894
Size: 61 270 563
Fragments arranged by size:
563 270 61
BstAPI GCAn_nnn'nTGC
Cuts at: 0 761 894
Size: 761 133
BstXI CCAn_nnnn'nTGG
Cuts at: 0 154 714 894
Size: 154 560 180
Fragments arranged by size:
560 180 154
BstYI r'GATC_y
Cuts at: 0 161 894
Size: 161 733
BtgI C'CryG_G
Cuts at: 0 33 535 894
Size: 33 502 359
Fragments arranged by size:
502 359 33
DraIII CAC_nnn'GTG
Cuts at: 0 495 894
Size: 495 399
EaeI y'GGCC_r
Cuts at: 0 289 801 894
Size: 289 512 93
Fragments arranged by size:
512 289 93
EarI CTCTTCn'nnn_
Cuts at: 0 191 471 894
Size: 191 280 423
Fragments arranged by size:
423 280 191
EcoNI CCTnn'n_nnAGG
Cuts at: 0 116 187 225 894
Size: 116 71 38 669
Fragments arranged by size:
669 116 71 38
EcoO109I rG'GnC_Cy
Cuts at: 0 4 232 387 531 894
Size: 4 228 155 144 363
Fragments arranged by size:
363 228 155 144 4
FspI TGC'GCA
Cuts at: 0 880 894
Size: 880 14
FspAI rTGC'GCAy
Cuts at: 0 880 894
Size: 880 14
HaeII r_GCGC'y
Cuts at: 0 87 212 894
Size: 87 125 682
Fragments arranged by size:
682 125 87
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 470 502 610 642 848 880 894
Size: 470 32 108 32 206 32 14
Fragments arranged by size:
470 206 108 32 32 32 14
KasI G'GCGC_C
Cuts at: 0 208 894
Size: 208 686
KpnI G_GTAC'C
Cuts at: 0 594 894
Size: 594 300
MslI CAynn'nnrTG
Cuts at: 0 152 894
Size: 152 742
MspA1I CmG'CkG
Cuts at: 0 174 294 449 516 726 840 894
Size: 174 120 155 67 210 114 54
Fragments arranged by size:
210 174 155 120 114 67 54
NaeI GCC'GGC
Cuts at: 0 333 894
Size: 333 561
NarI GG'CG_CC
Cuts at: 0 209 894
Size: 209 685
NgoMIV G'CCGG_C
Cuts at: 0 331 894
Size: 331 563
NspI r_CATG'y
Cuts at: 0 433 894
Size: 433 461
PflMI CCAn_nnn'nTGG
Cuts at: 0 519 894
Size: 519 375
PmlI CAC'GTG
Cuts at: 0 150 894
Size: 150 744
PpuMI rG'GwC_Cy
Cuts at: 0 4 531 894
Size: 4 527 363
Fragments arranged by size:
527 363 4
PstI C_TGCA'G
Cuts at: 0 136 395 740 839 856 894
Size: 136 259 345 99 17 38
Fragments arranged by size:
345 259 136 99 38 17
PvuII CAG'CTG
Cuts at: 0 174 449 516 726 840 894
Size: 174 275 67 210 114 54
Fragments arranged by size:
275 210 174 114 67 54
SanDI GG'GwC_CC
Cuts at: 0 531 894
Size: 531 363
SexAI A'CCwGG_T
Cuts at: 0 806 894
Size: 806 88
SfcI C'TryA_G
Cuts at: 0 132 391 736 835 852 894
Size: 132 259 345 99 17 42
Fragments arranged by size:
345 259 132 99 42 17
SfoI GGC'GCC
Cuts at: 0 210 894
Size: 210 684
SmlI C'TyrA_G
Cuts at: 0 691 894
Size: 691 203
SphI G_CATG'C
Cuts at: 0 433 894
Size: 433 461
StuI AGG'CCT
Cuts at: 0 122 894
Size: 122 772
StyI C'CwwG_G
Cuts at: 0 117 894
Size: 117 777
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 624 894
Size: 624 270
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 736 768 894
Size: 736 32 126
Fragments arranged by size:
736 126 32
Tth111I GACn'n_nGTC
Cuts at: 0 484 894
Size: 484 410
Enzymes that do cut:
AarI Acc65I AfeI AlfI AlwNI AvaI AvrII BanI
BanII BbeI BbsI BbvCI BglI Bme1580I BmgBI BmrI
BplI Bpu10I BpuEI BsaAI BsaHI BsaWI BsaXI BseRI
BseYI BsgI Bsp1286I BspMI BsrBI BsrFI BstAPI BstXI
BstYI BtgI DraIII EaeI EarI EcoNI EcoO109I FspI
FspAI HaeII Hin4I KasI KpnI MslI MspA1I NaeI
NarI NgoMIV NspI PflMI PmlI PpuMI PstI PvuII
SanDI SexAI SfcI SfoI SmlI SphI StuI StyI
TaqII TstI Tth111I
Enzymes that do not cut:
AatII AccI AclI AcuI AflII AflIII AgeI AhdI
AleI AloI ApaI ApaLI ApoI AscI AseI AsiSI
BaeI BamHI BcgI BciVI BclI BfrBI BglII BlpI
BmtI BpmI BsaI BsaBI BsiEI BsiHKAI BsiWI BsmI
BsmBI BspEI BspHI BsrDI BsrGI BssHII BssSI BstBI
BstEII BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI
DrdI EagI EciI EcoICRI Eco57MI EcoRI EcoRV FalI
FseI HincII HindIII HpaI MfeI MluI MmeI MscI
NcoI NdeI NheI NotI NruI NsiI PacI PasI
PciI PfoI PmeI PpiI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacI SacII SalI SapI SbfI
ScaI SfiI SgfI SgrAI SmaI SnaBI SpeI SrfI
SspI SwaI TatI XbaI XcmI XhoI XmaI XmnI
ZraI