Restriction map for
pFN21AE3616
(Linear) (Six-base) MAPSORT of: KIEE3616.seq Check: 7744 from: 1 to: 1434
KIEE3616.orf
With 182 enzymes: SgfI *
December 27, 2011 16:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 352 1434
Size: 352 1082
Acc65I G'GTAC_C
Cuts at: 0 1146 1434
Size: 1146 288
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 101 153 187 436 479 707 1162 1172
Size: 101 52 34 249 43 228 455 10
Cuts at: 1172 1434
Size: 262
Fragments arranged by size:
455 262 249 228 101 52 43 34
10
ApoI r'AATT_y
Cuts at: 0 768 1111 1248 1354 1434
Size: 768 343 137 106 80
Fragments arranged by size:
768 343 137 106 80
BanI G'GyrC_C
Cuts at: 0 18 1146 1434
Size: 18 1128 288
Fragments arranged by size:
1128 288 18
BanII G_rGCy'C
Cuts at: 0 858 1434
Size: 858 576
BbsI GAAGACnn'nnnn_
Cuts at: 0 167 721 1217 1434
Size: 167 554 496 217
Fragments arranged by size:
554 496 217 167
BglII A'GATC_T
Cuts at: 0 34 1137 1434
Size: 34 1103 297
Fragments arranged by size:
1103 297 34
Bme1580I G_kGCm'C
Cuts at: 0 307 1434
Size: 307 1127
BmrI ACTGGGnnnn_n'
Cuts at: 0 310 1434
Size: 310 1124
BmtI G_CTAG'C
Cuts at: 0 806 1434
Size: 806 628
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 852 1434
Size: 852 582
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 300 1274 1434
Size: 300 974 160
Fragments arranged by size:
974 300 160
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1202 1434
Size: 1202 232
BsaI GGTCTCn'nnnn_
Cuts at: 0 539 762 1434
Size: 539 223 672
Fragments arranged by size:
672 539 223
BsaAI yAC'GTr
Cuts at: 0 870 1434
Size: 870 564
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1247 1277 1434
Size: 1247 30 157
Fragments arranged by size:
1247 157 30
BseYI C'CCAG_C
Cuts at: 0 952 1434
Size: 952 482
BsiHKAI G_wGCw'C
Cuts at: 0 791 1434
Size: 791 643
BsmI GAATG_Cn'
Cuts at: 0 1004 1434
Size: 1004 430
Bsp1286I G_dGCh'C
Cuts at: 0 307 791 858 1434
Size: 307 484 67 576
Fragments arranged by size:
576 484 307 67
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 352 433 1434
Size: 352 81 1001
Fragments arranged by size:
1001 352 81
BsrDI GCAATG_nn'
Cuts at: 0 229 1434
Size: 229 1205
BstAPI GCAn_nnn'nTGC
Cuts at: 0 219 1434
Size: 219 1215
BstEII G'GTnAC_C
Cuts at: 0 388 1434
Size: 388 1046
BstYI r'GATC_y
Cuts at: 0 34 1137 1434
Size: 34 1103 297
Fragments arranged by size:
1103 297 34
BtgI C'CryG_G
Cuts at: 0 529 1434
Size: 529 905
DraI TTT'AAA
Cuts at: 0 107 1247 1434
Size: 107 1140 187
Fragments arranged by size:
1140 187 107
EaeI y'GGCC_r
Cuts at: 0 486 1434
Size: 486 948
EarI CTCTTCn'nnn_
Cuts at: 0 206 281 432 542 1229 1434
Size: 206 75 151 110 687 205
Fragments arranged by size:
687 206 205 151 110 75
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 101 153 187 300 436 479 707 1162
Size: 101 52 34 113 136 43 228 455
Cuts at: 1162 1172 1274 1434
Size: 10 102 160
Fragments arranged by size:
455 228 160 136 113 102 101 52
43 34 10
EcoO109I rG'GnC_Cy
Cuts at: 0 1239 1434
Size: 1239 195
HincII GTy'rAC
Cuts at: 0 242 1434
Size: 242 1192
HindIII A'AGCT_T
Cuts at: 0 689 1434
Size: 689 745
KpnI G_GTAC'C
Cuts at: 0 1150 1434
Size: 1150 284
MfeI C'AATT_G
Cuts at: 0 520 1434
Size: 520 914
MscI TGG'CCA
Cuts at: 0 488 1434
Size: 488 946
NcoI C'CATG_G
Cuts at: 0 529 1434
Size: 529 905
NheI G'CTAG_C
Cuts at: 0 802 1434
Size: 802 632
PfoI T'CCnGG_A
Cuts at: 0 660 1434
Size: 660 774
PmlI CAC'GTG
Cuts at: 0 870 1434
Size: 870 564
PpuMI rG'GwC_Cy
Cuts at: 0 1239 1434
Size: 1239 195
PsiI TTA'TAA
Cuts at: 0 398 1434
Size: 398 1036
SapI GCTCTTCn'nnn_
Cuts at: 0 432 1434
Size: 432 1002
SfcI C'TryA_G
Cuts at: 0 30 373 580 1434
Size: 30 343 207 854
Fragments arranged by size:
854 343 207 30
SmlI C'TyrA_G
Cuts at: 0 1217 1434
Size: 1217 217
SpeI A'CTAG_T
Cuts at: 0 508 1434
Size: 508 926
SspI AAT'ATT
Cuts at: 0 1125 1434
Size: 1125 309
StyI C'CwwG_G
Cuts at: 0 134 529 1434
Size: 134 395 905
Fragments arranged by size:
905 395 134
TatI w'GTAC_w
Cuts at: 0 1087 1434
Size: 1087 347
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 297 1434
Size: 297 1137
XmnI GAAnn'nnTTC
Cuts at: 0 62 1037 1434
Size: 62 975 397
Fragments arranged by size:
975 397 62
Enzymes that do cut:
AarI Acc65I AcuI ApoI BanI BanII BbsI BglII
Bme1580I BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaXI BseYI BsiHKAI BsmI Bsp1286I BspMI BsrDI BstAPI
BstEII BstYI BtgI DraI EaeI EarI Eco57MI EcoO109I
HincII HindIII KpnI MfeI MscI NcoI NheI PfoI
PmlI PpuMI PsiI SapI SfcI SmlI SpeI SspI
StyI TatI XcmI XmnI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BbeI BbvCI BcgI
BciVI BclI BfrBI BglI BlpI BmgBI Bpu10I BsaBI
BsaHI BsaWI BseRI BsgI BsiEI BsiWI BsmBI BspEI
BspHI BsrBI BsrFI BsrGI BssHII BssSI BstBI BstXI
BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraIII DrdI
EagI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI
FspI FspAI HaeII Hin4I HpaI KasI MluI MmeI
MslI MspA1I NaeI NarI NdeI NgoMIV NotI NruI
NsiI NspI PacI PasI PciI PflMI PmeI PpiI
PshAI PspOMI PspXI PsrI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SphI SrfI StuI
SwaI TaqII TstI Tth111I XbaI XhoI XmaI ZraI